Masayoshi Sakakura

Profile Information

Affiliation: Associate Professor, School of Science, Graduate School of Medical Life Science Department of Medical Life Science, Yokohama City University

Degree: 博士（薬学）(東京大学)

J-GLOBAL ID: 201201004766621952
researchmap Member ID: 7000002257

External link: http://www-mls.tsurumi.yokohama-cu.ac.jp/fsb/sakakura/

神経系に発現する膜タンパク質など、疾患に関連したタンパク質の構造解析・物理化学的性状解析を行っています。疾患の分子メカニズムを解明し、新規薬物を創製することを大きな目標にして、研究を進めています。

Research Interests

Research Areas

Life sciences / Pharmaceuticals - analytical and physicochemistry / structural biology

Research History

Apr, 2022 - Present

Yokohama City University
Apr, 2013 - Mar, 2022

Assistant Professor, Graduate School of Medical Life Science, Yokohama City University International College of Arts and Sciences Medical Life Science Graduate School of Medical Life Science Department of Medical Life Science
Apr, 2012 - Mar, 2013

Assistant Professor, Graduate School of Nanobioscience, Yokohama City University
Jun, 2011 - Mar, 2012

Research Assistant Professor, Graduate School of Nanobioscience, Yokohama City University
Apr, 2008 - May, 2011

Research Fellow, School of Medicine, Vanderbilt University
Jun, 2001 - Mar, 2008

Assistant Professor, Graduate School of Pharmaceutical Sciences, The University of Tokyo

Papers

Structural bases for the Charcot-Marie-Tooth disease induced by single amino acid substitutions of myelin protein zero.

Masayoshi Sakakura, Mikio Tanabe, Masaki Mori, Hideo Takahashi, Kazuhiro Mio

Structure (London, England : 1993), Aug 30, 2023
Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex

Bilge Argunhan, Masayoshi Sakakura, Negar Afshar, Misato Kurihara, Kentaro Ito, Takahisa Maki, Shuji Kanamaru, Yasuto Murayama, Hideo Tsubouchi, Masayuki Takahashi …

eLife, 9 e52566, Mar 24, 2020 Peer-reviewedLead author
Structural Mechanisms Underlying Activity Changes in an AMPA-type Glutamate Receptor Induced by Substitutions in Its Ligand-Binding Domain

Masayoshi Sakakura, Yumi Ohkubo, Hiraku Oshima, Suyong Re, Masahiro Ito, Yuji Sugita, Hideo Takahashi

Structure, 27(11) 1698-1709.e5, Nov, 2019 Peer-reviewedLead authorCorresponding author
Rad51 Interaction Analysis Reveals a Functional Interplay Among Recombination Auxiliary Factors

Bilge Argunhan, Masayoshi Sakakura, Negar Afshar, Misato Kurihara, Kentaro Ito, Takahisa Maki, Shuji Kanamaru, Yasuto Murayama, Hideo Tsubouchi, Masayuki Takahashi …

Aug 16, 2019
Peripheral myelin protein 22 modulates store-operated calcium channel activity, providing insights into Charcot-Marie-Tooth disease etiology

Carlos G. Vanoye, Masayoshi Sakakura, Rose M. Follis, Alexandra J. Trevisan, Malathi Narayan, Jun Li, Charles R. Sanders, Bruce D. Carter

Journal of Biological Chemistry, 294(32) 12054-12065, Aug 9, 2019 Peer-reviewed
Population Shift Mechanism for Partial Agonism of AMPA Receptor

Hiraku Oshima, Suyong Re, Masayoshi Sakakura, Hideo Takahashi, Yuji Sugita

BIOPHYSICAL JOURNAL, 116(1) 57-68, Jan, 2019 Peer-reviewed
Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system

Rika Suzuki, Masayoshi Sakakura, Masaki Mori, Moe Fujii, Satoko Akashi, Hideo Takahashi

Journal of Biomolecular NMR, 71(4) 1-11, Jun 5, 2018 Peer-reviewed
Structural mechanisms for the S‐nitrosylation‐derived protection of mouse galectin‐2 from oxidation‐induced inactivation revealed by NMR

Masayoshi Sakakura, Mayumi Tamura, Norihiko Fujii, Tomoharu Takeuchi, Tomomi Hatanaka, Seishi Kishimoto, Yoichiro Arata, Hideo Takahashi

The FEBS Journal, 285(6) 1129-1145, Mar, 2018 Peer-reviewedLead author
Allosteric activation of membrane-bound glutamate receptors using coordination chemistry within living cells

Shigeki Kiyonaka, Ryou Kubota, Yukiko Michibata, Masayoshi Sakakura, Hideo Takahashi, Tomohiro Numata, Ryuji Inoue, Michisuke Yuzaki, Itaru Hamachi

NATURE CHEMISTRY, 8(10) 958-967, Oct, 2016 Peer-reviewed
Structural Basis for the Trembler-J Phenotype of Charcot-Marie-Tooth Disease

Masayoshi Sakakura, Arina Hadziselimovic, Zhen Wang, Kevin L. Schey, Charles R. Sanders

Structure, 19(8) 1160-1169, Aug, 2011 Peer-reviewedLead author
Direct binding of cholesterol to the amyloid precursor protein: An important interaction in lipid-Alzheimer's disease relationships?

Beel AJ, Sakakura M, Barrett PJ, Sanders CR

Biochimica et biophysica acta, 1801(8) 975-982, Aug, 2010 Peer-reviewed
Lysophospholipid Micelles Sustain the Stability and Catalytic Activity of Diacylglycerol Kinase in the Absence of Lipids

Julia Koehler, Endah S. Sulistijo, Masayoshi Sakakura, Flak Jun Kim, Charles D. Ellis, Charles R. Sanders

BIOCHEMISTRY, 49(33) 7089-7099, Aug, 2010 Peer-reviewed
An NMR method for the determination of protein binding interfaces using TEMPOL-induced chemical shift perturbations

Jun Moriya, Masayoshi Sakakura, Yuji Tokunaga, R. Scott Prosser, Ichio Shimada

Biochimica et Biophysica Acta - General Subjects, 1790(10) 1368-1376, Oct, 2009 Peer-reviewed
Structural Investigation of the Interaction between LolA and LolB Using NMR

Shingo Nakada, Masayoshi Sakakura, Hideo Takahashi, Suguru Okuda, Hajime Tokuda, Ichio Shimada

JOURNAL OF BIOLOGICAL CHEMISTRY, 284(36) 24634-24643, Sep, 2009 Peer-reviewed
Cross-saturation and transferred cross-saturation experiments

Ichio Shimada, Takumi Ueda, Masahiko Matsumoto, Masayoshi Sakakura, Masanori Osawa, Koh Takeuchi, Noritaka Nishida, Hideo Takahashi

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 54(2) 123-140, Feb, 2009 Peer-reviewed
Carbohydrate binding mechanism of the macrophage galactose-type C-type lectin 1 revealed by saturation transfer experiments

Masayoshi Sakakura, Sarawut Oo-Puthinan, Chifumi Moriyama, Tomomi Kimura, Jun Moriya, Tatsuro Irimura, Ichio Shimada

Journal of Biological Chemistry, 283(48) 33665-33673, Nov 28, 2008 Peer-reviewedLead author
The amino acids involved in the distinct carbohydrate specificities between macrophage galactose-type C-type lectins 1 and 2 (CD301a and b) of mice

Sarawut Oo-puthinan, Keisuke Maenuma, Masayoshi Sakakura, Kaori Denda-Nagai, Makoto Tsuiji, Ichio Shimada, Sachiko Nakamura-Tsuruta, Jun Hirabayashi, Nicolai V. Bovin, Tatsuro Irimura

Biochimica et Biophysica Acta - General Subjects, 1780(2) 89-100, Feb, 2008 Peer-reviewed
Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli

Shingo Nakada, Masayoshi Sakakura, Hideo Takahashi, Hajime Tokuda, Ichio Shimada

BIOMOLECULAR NMR ASSIGNMENTS, 1(1) 121-123, Jul, 2007 Peer-reviewed
Backbone resonance assignments for the periplasmic chaperone LolA from Escherichia coli

Shingo Nakada, Hideo Takahashi, Masayoshi Sakakura, Masuo Kurono, Ichio Shimada

BIOMOLECULAR NMR ASSIGNMENTS, 1(1) 125-127, Jul, 2007 Peer-reviewed
Thermodynamic and structural basis for transition-state stabilization in antibody-catalyzed hydrolysis

Masayuki Oda, Nobutoshi Ito, Takeshi Tsumraya, Kayo Suzuki, Masayoshi Sakakura, Ikuo Fujii

JOURNAL OF MOLECULAR BIOLOGY, 369(1) 198-209, May, 2007 Peer-reviewed

Misc.

ガレクチン-2のS-ニトロソ化部位のLC-MS/MSによる解析

田村真由美, 藤井智彦, 坂倉正義, 武内智春, 畑中朋美, 高橋栄夫, 岸本成史, 荒田洋一郎

日本薬学会年会要旨集, 139年会(3) 102-102, Mar, 2019
Effects of T686A Mutation on the Structural Stability of the AMPA Receptor Ligand-Binding Domain

Hiraku Oshima, Suyong Re, Masayoshi Sakakura, Hideo Takahashi, Yuji Sugita

BIOPHYSICAL JOURNAL, 114(3) 125A-125A, Feb, 2018
NMRを用いたS-ニトロソ化によるガレクチン-2の酸化的失活防御メカニズムの解明

坂倉正義, 田村真由美, 武内智春, 荒田洋一郎, 高橋栄夫

生命科学系学会合同年次大会, 2017年度 [1AT26-09(1P, Dec, 2017
Structural analysis of catalytic antibodies

Sakakura M., Shimba N., Takahashi H., Arata Y., Fujii I., Shimada I.

Biophysics, 38(2) S44, Sep 7, 1998
NMR study of the active site of catalytic antibody

SAKAKURA M., SHIMBA N., TAKAHASHI H., TANAKA F., FUJII I., SHIMADA I.

Biophysics, 37 S21, Oct, 1997

Books and Other Publications

NMRによる有機材料分析とその試料前処理、データ解釈

技術情報協会 (Role: Joint author, 第６章６節 NMRによるペプチドの立体構造解析)

技術情報協会, Sep, 2021 (ISBN: 9784861048609)
物理系薬学〈3〉生体分子・化学物質の構造決定 (スタンダード薬学シリーズ)

日本薬学会 (Role: Contributor, SBO 16)

東京化学同人, Feb 1, 2006 (ISBN: 4807914537)

Research Projects

Structural analyses of PMP22 and MPZ to elucidate the molecular mechanism of Charcot-Marie-Tooth disease

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2024
Redox sensing by membrane proteins via cysteine residues in the TM region as studied by using solution NMR

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2022

Tokunaga Yuji
Structural analyses of PMP22 and MPZ to reveal the molecular basis of Charcot-Marie-Tooth disease

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Sakakura Masayoshi
NMR approach for the functional structural analyses of human membrane proteins

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

Takahashi Hideo, SAKAKURA Masayoshi
Structural analyses of MAPEG family membrane proteins using NMR

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

Sakakura Masayoshi, FUJII Moe, FUSHIMI Taketoshi, KOIKE Kenichiro, SUZUKI Rika
Fundamental research for NMR structural analysis of human membrane proteins

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

TAKAHASHI Hideo, TAKEUCHI Koh, SAKAKURA Masayoshi
Study of an AMPAR auxiliary transmembrane protein, stargazin

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2014

SAKAKURA Masayoshi
コンタクトシフトと立体整列同位体標識法を利用した新規蛋白質相互作用解析法の開発

科学研究費助成事業若手研究(B), 若手研究(B), 日本学術振興会, 2007 - 2008

坂倉正義
NMRを用いた生体機能分子-標的蛋白質複合体の相互作用解析法の開発

科学研究費助成事業特定領域研究, 特定領域研究, 日本学術振興会, 2006 - 2007

坂倉正義, 嶋田一夫
酸素分子を利用した生体高分子複合体の相互作用界面を決定する新規NMR解析法の開発

科学研究費助成事業若手研究(B), 若手研究(B), 日本学術振興会, 2005 - 2006

坂倉正義
NMRを用いた生体機能分子・標的蛋白質複合体の相互作用解析法の開発

科学研究費助成事業特定領域研究, 特定領域研究, 日本学術振興会, 2005 - 2005

坂倉正義, 嶋田一夫
原子レベルでの病態解明に向けたハイスループットな分子間相互作用解析法の開発

科学研究費助成事業特定領域研究, 特定領域研究, 日本学術振興会, 2003 - 2003

嶋田一夫, 坂倉正義

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Research Areas

Research History

Papers

Misc.

Books and Other Publications

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