Kazuhisa Nakayama

Profile Information

Affiliation: Kyoto University

Degree: 医学博士(京都大学)

ORCID ID: https://orcid.org/0000-0001-7701-7183
J-GLOBAL ID: 201401049082985937
researchmap Member ID: 7000008311

External link: http://www.pharm.kyoto-u.ac.jp//physchem/

Research Areas

Life sciences / Cell biology /

Research History

May, 2003 - Present

Professor, Graduate School of Pharmaceutical Sciences, Kyoto University
Apr, 2002 - Apr, 2003

Professor, Faculty of Pharmaceutical Sciences, Kanazawa University
Jan, 1994 - Mar, 2002

Associate Professor, Institute of Biological Sciences, University of Tsukuba
Apr, 1987 - Jan, 1994

Assistant Professor, Institute of Biological Sciences, University of Tsukuba

Education

Apr, 1983 - Mar, 1987

Graduate School of Medicine, Kyoto University
Apr, 1981 - Mar, 1983

Graduate School of Pharmaceutical Sciences, Kyoto University
Apr, 1977 - Mar, 1981

Faculty of Pharmaceutical Sciences, Kyoto University

Awards

2017

JB論文賞, Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis. (2016) J. Biochem., 159, 201-208., 日本生化学会

Hanai, A, Ohgi, M, Yagi, C, Ueda, T, Shin, H.-W, Nakayama, K
Oct, 2013

Best Teacher Award
2013

CSF Award, Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes. (2013) Cell Struct. Funct., 38, 31-41., 日本細胞生物学会

Takatsu, H, Katoh, Y, Ueda, T, Waguri, S, Murayama, T, Takahashi, S, Shin, H.-W, Nakayama, K
2001

JB論文賞, Identification and characterization of novel isoforms of COP I subunits. (2000) J. Biochem., 128, 793-801., 日本生化学会

Futatsumori, M, Kasai, K, Takatsu, H, Shin, H.-W, Nakayama, K
1994

日本生化学会奨励賞, 生理活性ペプチドおよびタンパク質前駆体のプロセシング酵素に関する研究, 日本生化学会

中山和久

Papers

228

Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease

Asuka Hamamoto, Natsuki Kita, Siddabasave Gowda B. Gowda, Hiroyuki Takatsu, Kazuhisa Nakayama, Makoto Arita, Shu-Ping Hui, Hye-Won Shin

Cell Structure and Function, 49(1) 1-10, 2024 Peer-reviewed
Compound heterozygous IFT81 variations in a skeletal ciliopathy patient cause Bardet-Biedl syndrome-like ciliary defects.

Koshi Tasaki, Zhuang Zhou, Yamato Ishida, Yohei Katoh, Kazuhisa Nakayama

Human molecular genetics, 32 2887-2900, Jul 10, 2023 Peer-reviewedLast authorCorresponding author
Novel phosphatidylinositol flippases contribute to phosphoinositide homeostasis in the plasma membrane

Yumeka Muranaka, Ryo Shigetomi, Yugo Iwasaki, Asuka Hamamoto, Kazuhisa Nakayama, Hiroyuki Takatsu, Hye-Won Shin

Jul 3, 2023
Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex.

Shunya Hiyamizu, Hantian Qiu, Yuta Tsurumi, Yuki Hamada, Yohei Katoh, Kazuhisa Nakayama

Biology Open, Jun 13, 2023 Peer-reviewedLast authorCorresponding author
Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport.

Shunya Hiyamizu, Hantian Qiu, Laura Vuolo, Nicola L Stevenson, Caroline Shak, Kate J Heesom, Yuki Hamada, Yuta Tsurumi, Shuhei Chiba, Yohei Katoh …

Journal of Cell Science, 136(5), Mar 1, 2023 Peer-reviewedLast authorCorresponding author
Disease-associated mutations in WDR34 lead to diverse impacts on the assembly and function of dynein-2

Caroline Shak, Laura Vuolo, Borhan Uddin, Yohei Katoh, Tom Brown, Aakash G. Mukhopadhyay, Kate Heesom, Anthony J. Roberts, Nicola Stevenson, Kazuhisa Nakayama …

Journal of Cell Science, 136(5), Mar 1, 2023 Peer-reviewed
CEP19-RABL2-IFT-B axis controls BBSome-mediated ciliary GPCR export.

Zhuang Zhou, Yohei Katoh, Kazuhisa Nakayama

Molecular biology of the cell, 33(13) ar126, Sep 8, 2022 Peer-reviewedLast authorCorresponding author
Molecular basis underlying the ciliary defects caused by IFT52 variations found in skeletal ciliopathies.

Yamato Ishida, Koshi Tasaki, Yohei Katoh, Kazuhisa Nakayama

Molecular biology of the cell, 33(9) ar83, Jun 15, 2022 Peer-reviewedLast authorCorresponding author
BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to IFT turnaround involving ICK/CILK1.

Yuuki Satoda, Tatsuro Noguchi, Taiju Fujii, Aoi Taniguchi, Yohei Katoh, Kazuhisa Nakayama

Molecular biology of the cell, 33(9) ar79, May 24, 2022 Peer-reviewedLast authorCorresponding author
Impaired cooperation between IFT74/BBS22–IFT81 and IFT25–IFT27/BBS19 causes Bardet-Biedl syndrome

Zhuang Zhou, Hantian Qiu, Roiner-Francisco Castro-Araya, Ryota Takei, Kazuhisa Nakayama, Yohei Katoh

Human Molecular Genetics, 31(10) 1681-1693, May 15, 2022 Peer-reviewedCorresponding author
Combinations of deletion and missense variations of the dynein-2 DYNC2LI1 subunit found in skeletal ciliopathies cause ciliary defects.

Hantian Qiu, Yuta Tsurumi, Yohei Katoh, Kazuhisa Nakayama

Scientific reports, 12(1) 31-31, Jan 7, 2022 Peer-reviewedLast authorCorresponding author
CCRK/CDK20 regulates ciliary retrograde protein trafficking via interacting with BROMI/TBC1D32

Tatsuro Noguchi, Kentaro Nakamura, Yuuki Satoda, Yohei Katoh, Kazuhisa Nakayama

PLOS ONE, 16(10) e0258497, Oct 8, 2021 Peer-reviewedLast authorCorresponding author
The interaction of ATP11C-b with ezrin contributes to its polarized localization

Inoue, H., Takatsu, H., Hamamoto, A., Takayama, M., Nakabuchi, R., Muranaka, Y., Yagi, T., Nakayama, K., Shin, H.-W.

Journal of Cell Science, 134(20) jcs258523., Sep 16, 2021 Peer-reviewed
ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane

Sayaka Fujisawa, Hantian Qiu, Shohei Nozaki, Shuhei Chiba, Yohei Katoh, Kazuhisa Nakayama

Biology Open, 10(9) bio058843, Aug 27, 2021 Peer-reviewedLast authorCorresponding author
Molecular basis of ciliary defects caused by compound heterozygous IFT144/WDR19 mutations found in cranioectodermal dysplasia

Yamato Ishida, Takuya Kobayashi, Shuhei Chiba, Yohei Katoh, Kazuhisa Nakayama

Human Molecular Genetics, 30(3-4) 213-225, Jan 30, 2021 Peer-reviewedLast authorCorresponding author
Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry

Hantian Qiu, Sayaka Fujisawa, Shohei Nozaki, Yohei Katoh, Kazuhisa Nakayama

Biology Open, 10(1) bio057653, Jan 15, 2021 Peer-reviewedLast authorCorresponding author
Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import

Takuya Kobayashi, Yamato Ishida, Tomoaki Hirano, Yohei Katoh, Kazuhisa Nakayama

Molecular Biology of the Cell, 32(1) 45-56, Jan 1, 2021 Peer-reviewedLast authorCorresponding author
Anterograde trafficking of ciliary MAP kinase–like ICK/CILK1 by the intraflagellar transport machinery is required for intraciliary retrograde protein trafficking

Kentaro Nakamura, Tatsuro Noguchi, Mariko Takahara, Yoshihiro Omori, Takahisa Furukawa, Yohei Katoh, Kazuhisa Nakayama

Journal of Biological Chemistry, 295(38) 13363-13376, Sep 18, 2020 Peer-reviewedLast authorCorresponding author
Formation of the B9-domain protein complex MKS1–B9D2–B9D1 is essential as a diffusion barrier for ciliary membrane proteins

Misato Okazaki, Takuya Kobayashi, Shuhei Chiba, Ryota Takei, Luxiaoxue Liang, Kazuhisa Nakayama, Yohei Katoh

Molecular Biology of the Cell, 31(20) 2259-2268, Sep 15, 2020 Peer-reviewedCorresponding author
Practical method for superresolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification

Yohei Katoh, Shuhei Chiba, Kazuhisa Nakayama

Molecular Biology of the Cell, 31(20) 2195-2206, Sep 15, 2020 Peer-reviewedLast author

Misc.

Imaging of cilia and centrosome by Expansion Microscopy

千葉秀平, 加藤洋平, 中山和久

Bio Clinica, 37(11), 2022
Observation of primary cilia and centrioles by expansion microscopy

加藤洋平, 千葉秀平, 中山和久

実験医学, 40(16), 2022
Superresolution imaging of primary cilia by expansion microscopy using an amplibody for fluorescence signal amplification

加藤洋平, 千葉秀平, 中山和久

生化学, 94(4), 2022
繊毛形成と繊毛内タンパク質輸送の分子基盤

中山和久, 加藤洋平, 千葉秀平

日本生化学会大会(Web), 94th, 2021
Super-resolution imaging of structure and assembly mechanism of the ciliary transition zone in mammalian cells.

千葉秀平, 加藤洋平, 中山和久

日本分子生物学会年会プログラム・要旨集(Web), 44th, 2021
膨張顕微鏡法を用いた一次繊毛の超解像イメージング

加藤洋平, 千葉秀平, 中山和久

日本生化学会大会(Web), 90th, 2017
Visible immunoprecipitation assay: a convenient and versatile method for studying protein–protein interactions using fluorescent fusion proteins

Yohei Katoh, Kazuhisa Nakayama

Experimental Medicine, 35(1) 89-95, Jan, 2017 Invited
Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins Arl13b and INPP5E

S. Nozaki, Y. Katoh, K. Nakayama

MOLECULAR BIOLOGY OF THE CELL, 27, 2016
Phospholipid flippase ATP10A, a member of type IV P-type ATPases, translocates phosphatidylcholine and is involved in plasma membrane dynamics.

T. Naito, H. Takatsu, R. Miyano, N. Takada, K. Nakayama, H. Shin

MOLECULAR BIOLOGY OF THE CELL, 27, 2016
Architectures of the BBSome, IFT-B, and Exocyst Complexes Revealed by Visible Immunoprecipitation (VIP) Assay Using Fluorescent Fusion Proteins

Kazuhisa Nakayama

FASEB Science Research Conference, “Biology o f Cilia and Flagella”, Jul, 2015
リサイクリングエンドソームを介する輸送経路におけるタンパク質とリン脂質の協調的作用．

日本生化学会, Sep, 2013
Roles of Arf and Rab GTPases in cytokinesis.

Nakayama,K

FASEB, Jul, 2013
Membrane remodeling in post-Golgi trafficking.

日本細胞生物学会, Jun, 2013
ゴルジ体に局在する低分子量GTPase ARFの機能の再考

第84回日本生化学会大会シンポジウム, Sep, 2011
Mitosis-Coupled Sequestration of Recycling Endosomes around Spindle Pole Centrosomes

第33回日本分子生物学会年会・第83回日本生化学会大会合同大会シンポジウム, Dec, 2010
紡錘体極中心体のまわりに形成されるリサイクリングエンドソームのクラスター．

第115回日本解剖学会全国学術集会シンポジウム, Mar, 2010
RabファミリーとArfファミリーの低分子量GTPaseによる細胞質分裂の調節

第82回日本生化学会大会シンポジウム, Oct, 2009
タンパク質の細胞膜への輸送とエンドサイトーシスの調節

第82回日本薬理学会年会シンポジウム, Mar, 2009
低分子量GTPaseによるリサイクリングエンドソーム機能と細胞質分裂の調節

第31回日本分子生物学会年会・第81回日本生化学会大会合同大会シンポジウム, Dec, 2008
FIP3、FIP4によるRab11とARFの機能のクロストーク

日本分子生物学会2006 フォーラムシンポジウム, Dec, 2006

Books and Other Publications

レーニンジャーの新生化学：生化学と分子生物学の基本原理（第7版下）

中山和久 (Role: Editor)

廣川書店, May, 2019
レーニンジャーの新生化学：生化学と分子生物学の基本原理（第7版上）

中山和久 (Role: Editor)

廣川書店, May, 2019
くすりをつくる研究者の仕事：薬のタネ探しから私たちに届くまで

中山和久

化学同人, Mar, 2017
Arfファミリーによるメンブレントラフィックの調節．メンブレントラフィック：膜・小胞による細胞内輸送ネットワーク

中山和久

化学同人, Jul, 2016
レーニンジャーの新生化学：生化学と分子生物学の基本原理（第６版下）

中山和久 (Role: Editor)

廣川書店, Mar, 2015 (ISBN: 4567244079)
レーニンジャーの新生化学：生化学と分子生物学の基本原理（第６版上）

中山和久 (Role: Editor)

廣川書店, Mar, 2015 (ISBN: 4567244060)
基礎コース細胞生物学

中山和久 (Role: Joint translator, 18. 機械的分子)

東京化学同人, Sep, 2013 (ISBN: 4807908197)
プロッパー細胞生物学：細胞の基本原理を学ぶ

中山和久 (Role: Editor)

化学同人, Mar, 2013 (ISBN: 4759815333)
ベーシック薬学教科書シリーズ生化学

中山和久 (Role: Joint author, ２章細胞の構造と細胞小器官の役割)

化学同人, Apr, 2012
遺伝子医学MOOK20：ナノバイオ技術と最新創薬応用研究

中山和久 (Role: Joint author, メンブレントラフィックによる細胞分裂制御機構の解明)

メディカルドゥ, Jan, 2012
レーニンジャーの新生化学（第５版上，下）

中山和久編集 (Role: Editor)

廣川書店, 2010

Presentations

繊毛内タンパク質輸送複合体の構築様式と機能．

中山和久

第41回蛋白質の酵素の構造と機能に関する九州シンポジウム, Sep, 2016, 日本生化学会
Trafficking Machineries within Cilia: Arc hitectures and Functions of the IFT-A and IFT-B complexes.

中山和久

68回日本細胞生物学会大会シンポジウム「高次生命機能を司るメンブレントラフィック：分子基盤からその破綻による疾患発症の理解に向けて」, Jun, 2016, 日本細胞生物学会
Regulation of cell division by membrane traffic involving Rab and Arf families of small GTPases

Kazuhisa Nakayama

International Symposium: Epigenetic Landscape in Biological Rhythms, “RNA and Clock”, Mar, 2015
Architecture of multi-subunit complexes involved in membrane trafficking revealed by visible immunopreci pitation (VIP) assay.

Kazuhisa Nakayama

The 18th iCeMS International Symposium/The 15th International Membrane Research Forum., Mar, 2015
メンブレントラフィックと連動する動物細胞の細胞質分裂

中山和久

第66回日本細胞生物学会大会シンポジウム, Jun, 2014, 日本細胞生物学会
メンブレントラフィックと共役する多様な細胞機能の調節．

中山和久

第80回日本生化学会東北支部例会シンポジウム, May, 2014, 日本生化学会
Regulation of cytokinesis coupled with membrane traffic.

Kazuhisa Nakayama

Regulation of cytokinesis coupled with membrane traffic. International Symposium of Insect Biomedical Research Center in KIT,, 2014
メンブレントラフィックと共役する細胞分裂の調節

中山和久

第86回日本生化学会大会シンポジウム, Sep, 2013, 日本生化学会
細胞分裂におけるメンブレントラフィックの役割：細胞生物学と構造生物学の融合研究

中山和久

第１回物構研サイエンスフェスタ, Mar, 2013, KEK 物質構造科学研究所
細胞分裂時のオルガネラ分配の調節

中山和久

千里ライフサイエンスセミナー：生命科学を支えるオルガネラ研究の新展開, Sep, 2011, 千里ライフサイエンス振興財団
Regulation of Arf6 and Rab11 during cell division

FASEB Summer Research Conference, “Arf Family G Proteins”. Carefree, Aug, 2010, FASEB
Positive and negative regulation of membrane traffic during cell division.

緒方洪庵生誕200周年記念国際シンポジウム, Jul, 2010, 大阪大学
ARFRP1 and ARL1 independently regulate membrane trafficking ‘from’ and ‘to’ the TGN.

International Symposium on Membrane Traffic, Sep, 2007
Distinct and redundant functions of BIG1 and BIG2 in TGN and recycling endosome

FASEB Summer Research/CNRS Conference “Arf Family GTPases”., Jun, 2007, FASEB
Regulation of membrane trafficking between the TGN and endosomes by ARF-GEFs. 20th IUBMB International Congress of Biochemistry and Molecular Biology

Symposium "Regulating Membrane Traffic to Endosomes/Lysosomes", Jun, 2006, 日本分子生物学会
BIG2, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factors: Its Function at the Recycling Endosome.

第78回日本生化学会大会ミニシンポジウム, Oct, 2005, 日本生化学会
GGAs: Traffic controllers at the TGN crossroads.

International Symposium “Membrane Traffic”, Jan, 2005
GGAタンパク質：TGN-エンドソーム間の輸送を制御するクラスリン・アダプター

公開シンポジウム「蛋白質分解：新たなる展開をめざして」．, Sep, 2004, 特定領域研究：蛋白質分解－新しいモディファイアー蛋白質による制御－
Regulation of membrane trafficking by ARF-GEFs.

Third International Symposium on Autophagy., Sep, 2002
Functions of BIG2 and GBF1, Guanine-nucleotide Exchange factors for ARFs.

Symposium “Molecular Mechanisms of Intracellular Membrane Traffic”, Jan, 2002

Research Projects

Relationship between architecture and function of intraciliary protein trafficking complex: molecular basis for ciliopathies

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2024
Architecture of the transition zone separating the cytoplasm and cilioplasmand, and selective protein translocation across it

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2022
Regulation of vesicular trafficking by tethering complex and SNARE proteins

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

Nakayama Kazuhisa, KATOH Yohei
タンパク質間相互作用解析とイメージングに基づく繊毛形成と繊毛内輸送機構の解明

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2015 - Mar, 2017

中山和久
Development of high-throughput system of visible immunoprecipitation assay

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2017

Nakayama Kazuhisa, KATOH Yohei
生細胞でのイメージングを基盤とするシリアの形成とタンパク質輸送機構の解明

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2013 - Mar, 2015

中山和久
Coupling of membrane traffic and cytoskeleton remodeling in the regulation of cell motility

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2010 - 2012

NAKAYAMA Kazuhisa
ARF-COPI輸送系が関与する新たな細胞機能の解析

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, 2009 - 2010

中山和久
細胞質分裂の調節における低分子量GTPaseとそのエフェクターの役割

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2008 - 2009

中山和久
Regulation of membrane traffic and ubiquitination by small GTPases

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 2006 - 2008

NAKAYAMA Kazuhisa
「メンブレントラフィック-分子機構から高次機能への展開」の取りまとめ

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2003 - 2008

大野博司, 中野明彦, 吉森保, 中山和久, 和田洋
ゴルジ体を中心とするメンブレントラフィックの制御機構

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2003 - 2007

中山和久, 申恵媛
Roles of multifunctional adaptor proteins, GGAs, in membrane traffic

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, 2003 - 2005

NAKAYAMA Kazuhisa, SHIN Hye-Won
ARFおよびARLの活性化定量法および細胞内局在決定法の開発

科学研究費助成事業, 萌芽研究, 日本学術振興会, 2004 - 2004

中山和久
活性型ARFの定量法および細胞内局在決定法の開発

科学研究費助成事業, 萌芽研究, 日本学術振興会, 2003 - 2003

中山和久
軸索伸長におけるRho-mDiaを介する低分子量G蛋白質間の協調作用の解明

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2001 - 2002

富永知子, 中山和久
低分子量GTP結合蛋白質ARFの活性型の定量法の開発

科学研究費助成事業, 萌芽的研究, 日本学術振興会, 2001 - 2001

中山和久
細胞運動およびがん化におけるRho-mDia-Src系の機能と作用機構の解明

科学研究費助成事業, 特定領域研究(C), 日本学術振興会, 2001 - 2001

富永知子, 中山和久
Roles of clathrin-adaptor-like proteins in intracellular protein traffic.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas (B), Japan Society for the Promotion of Science, 1998 - 2001

NAKAYAMA Kazuhisa, OHNO Hiroshi
ROLES OF GTP-BINDING PROTEINS IN THE FORMATION OF INTRACELLULAR TRANSPORT VESICLES

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B)., Japan Society for the Promotion of Science, 1997 - 2000

NAKAYAMA Kazuhisa

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