Atsushi Miyawaki

Profile Information

Affiliation: RIKEN

J-GLOBAL ID: 201601009027269053
researchmap Member ID: B000264073

Research Areas

Life sciences / Biophysics /

Papers

363

Secretory GFP reconstitution labeling of neighboring cells interrogates cell–cell interactions in metastatic niches

Misa Minegishi, Takahiro Kuchimaru, Kaori Nishikawa, Takayuki Isagawa, Satoshi Iwano, Kei Iida, Hiromasa Hara, Shizuka Miura, Marika Sato, Shigeaki Watanabe …

Nature Communications, 14(1), Dec 5, 2023
StayGold variants for molecular fusion and membrane-targeting applications

Ryoko Ando, Satoshi Shimozono, Hideo Ago, Masatoshi Takagi, Mayu Sugiyama, Hiroshi Kurokawa, Masahiko Hirano, Yusuke Niino, Go Ueno, Fumiyoshi Ishidate …

Nature Methods, Nov 30, 2023
StayGold Photostability under Different Illumination Modes

Masahiko Hirano, Yasuo Yonemaru, Satoshi Shimozono, Mayu Sugiyama, Ryoko Ando, Yasushi Okada, Takahiro Fujiwara, Atsushi Miyawaki

Nov 23, 2023
Knockout of all ErbB-family genes delineates their roles in proliferation, survival, and migration.

Kimiya Matsuda, Daiki Hirayama, Naoya Hino, Sota Kuno, Asako Sakaue-Sawano, Atsushi Miyawaki, Michiyuki Matsuda, Kenta Terai

Journal of cell science, Jul 31, 2023
A highly photostable and bright green fluorescent protein.

Masahiko Hirano, Ryoko Ando, Satoshi Shimozono, Mayu Sugiyama, Noriyo Takeda, Hiroshi Kurokawa, Ryusaku Deguchi, Kazuki Endo, Kei Haga, Reiko Takai-Todaka …

Nature biotechnology, 40(7) 1132-1142, Apr 25, 2022
Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Takayuki Michikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Applied Physics Express, 15 042005-1-042005-5, Mar 28, 2022 Peer-reviewed
Adaptive optics with spatio-temporal lock-in detection for temporal focusing microscopy

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Optics Express, 29(18) 29021-29021, Aug 30, 2021
Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing

Kenta Inazawa, Keisuke Isobe, Tomohiro Ishikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

Applied Physics Express, 14(8) 082008-082008, Aug 1, 2021
Development of phenyl oligoene-type firefly luciferin analogues with extended π-electronic conjugation for near-infrared bioluminescence

Genta Kamiya, Nobuo Kitada, Ryohei Saito-Moriya, Rika Obata, Satoshi Iwano, Atsushi Miyawaki, Takashi Hirano, Shojiro Maki

Chemistry Letters, 50(8) 1523-1525, May 20, 2021 Peer-reviewed
Metastatic Single Tumor Cells Evade NK Cell-mediated Killing by Thrombin-mediated Loss of the Activating Ligand CD155/PVR/Necl-5

Hiroshi Ichise, Shoko Tsukamoto, Tsuyoshi Hirashima, Yoshinobu Konishi, Choji Oki, Shinya Tsukiji, Satoshi Iwano, Atsushi Miyawaki, Kenta Sumiyama, Kenta Terai …

Jan 17, 2021
A hypothalamic novelty signal modulates hippocampal memory.

Shuo Chen, Linmeng He, Arthur J Y Huang, Roman Boehringer, Vincent Robert, Marie E Wintzer, Denis Polygalov, Adam Z Weitemier, Yanqiu Tao, Mingxiao Gu …

Nature, 586(7828) 270-274, Oct, 2020
Distributed sensory coding by cerebellar complex spikes in units of cortical segments

Takayuki Michikawa, Takamasa Yoshida, Satoshi Kuroki, Takahiro Ishikawa, Shinji Kakei, Shigeyoshi Itohara, Atsushi Miyawaki

Sep 18, 2020
Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact.

Nagatoki Kinoshita, Arthur J Y Huang, Thomas J McHugh, Atsushi Miyawaki, Tomomi Shimogori

Scientific reports, 10(1) 14437-14437, Sep 2, 2020
Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration.

Hiroyuki Katayama, Hiroshi Hama, Koji Nagasawa, Hiroshi Kurokawa, Mayu Sugiyama, Ryoko Ando, Masaaki Funata, Nobuyo Yoshida, Misaki Homma, Takanori Nishimura …

Cell, 181(5) 1176-1187, May 28, 2020 Peer-reviewed
Presynaptic MAST kinase controls opposing postsynaptic responses to convey stimulus valence in Caenorhabditis elegans

Shunji Nakano, Muneki Ikeda, Yuki Tsukada, Xianfeng Fei, Takamasa Suzuki, Yusuke Niino, Rhea Ahluwalia, Ayana Sano, Rumi Kondo, Kunio Ihara …

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 117(3) 1638-1647, Jan, 2020
G9a-dependent histone methylation can be induced in G1 phase of cell cycle.

Fukuda M, Sakaue-Sawano A, Shimura C, Tachibana M, Miyawaki A, Shinkai Y

Scientific reports, 9(1) 956, Jan, 2019 Peer-reviewed
Infrared spectroscopy reveals multi-step multi-timescale photoactivation in the photoconvertible protein archetype dronpa

Sergey P. Laptenok, Agnieszka A. Gil, Christopher R. Hall, Andras Lukacs, James N. Iuliano, Garth A. Jones, Gregory M. Greetham, Paul Donaldson, Atsushi Miyawaki, Peter J. Tonge …

Nature Chemistry, 10(8) 1-8, Jun 11, 2018 Peer-reviewed
A spherical aberration-free microscopy system for live brain imaging

Yoshihiro Ue, Hiromu Monai, Kaori Higuchi, Daisuke Nishiwaki, Tetsuya Tajima, Kenya Okazaki, Hiroshi Hama, Hajime Hirase, Atsushi Miyawaki

Biochemical and Biophysical Research Communications, 500(2) 236-241, Jun 2, 2018 Peer-reviewed
Optical clearing of the pancreas for visualization of mature β-cells and vessels in mice.

Nishimura W, Sakaue-Sawano A, Takahashi S, Miyawaki A, Yasuda K, Noda Y

Islets, 10(3) e1451282, May, 2018 Peer-reviewed
Interferometric temporal focusing microscopy using three-photon excitation fluorescence

Keisuke Toda, Keisuke Isobe, Kana Namiki, Hiroyuki Kawano, Atsushi Miyawaki, Katsumi Midorikawa

Biomedical Optics Express, 9(4) 1510-1519, Apr 1, 2018 Peer-reviewed

Misc.

Fabrication of microfluidic biochip using amorphas fluor polymer and application to high-resolution cell observation

半澤未来, 半澤未来, 小幡孝太郎, 河野弘幸, 尾笹一成, 花田修賢, 宮脇敦史, 宮地悟代, 杉岡幸次

応用物理学会春季学術講演会講演予稿集(CD-ROM), 70th, 2023
動物にやさしいin vivo発光イメージング

岩野智, 牧昌次郎, 宮脇敦史

実験医学別冊発光イメージング実験ガイド, 182-187, Oct, 2019 Invited
AkaBLI

Iwano Satoshi, Maki Shojiro, Atsushi Miyawaki

70(3) 244-251, Jun, 2019 Invited
Why not in vivo BLI?

Iwano Satoshi, Maki Shojiro, Atsushi Miyawaki

36(19 (12月号)) 3273-3281, Dec, 2018 Invited
マウスの脳の二光子顕微鏡画像からの神経細胞認識処理の開発

君塚涼, 道川貴章, 竹本智子, 横田秀夫, 宮脇敦史, 清水昭伸

日本医用画像工学会大会予稿集(CD-ROM), 37th, 2018
Tracking Normal and Chronic Myeloid Leukemia Progenitor Cell Cycle Kinetics in a Defined Niche Using Live Time Lapse Confocal Imaging with Fucci2BL a Novel Lentiviral Bicistronic Reporter

Gabriel Pineda, Kathleen M. Lennon, Nathaniel P. Delos-Santos, Florence Lambert-Fliszar, Gennarina L. Riso, Marco A. Marra, Sheldon Morris, Asako Sakaue-Sawano, Atsushi Miyawaki, Catriona H. M. Jamieson

BLOOD, 126(23), Dec, 2015
細胞内で結晶化する蛍光タンパク質

筒井秀和, 宮脇敦史

現代化学, (535) 42-46, Oct, 2015
A bilirubin-inducible fluorescent protein from eel muscle

Akiko Kumagai, Atsushi Miyawaki

NIPPON SUISAN GAKKAISHI, 81(4) 730-730, Jul, 2015
Visualizing cell-cycle progression

14(2) 121-129, May, 2015
R26Fucci2aR: A global Cre-inducible cell cycle reporter mouse allowing live imaging of cell cycle progression during mouse embryonic development

Richard Mort, Matthew Ford, Asako Sakaue-Sawano, Angela Casadio, Adam Douglas, Margaret Keighren, Nils Lindstrom, Peter Hohenstein, Atsushi Miyawaki, Ian Jackson

TRANSGENIC RESEARCH, 23(5) 855-855, Oct, 2014
CS4-01 Observing heterogeneity in cell society(CS4 New Development of fluorescent probes,CJJSHC Symposium) :

Sakaue-Sawano Asako, Miyawaki Atsushi

(55) 116-116, 2014
ニホンウナギ筋肉由来のタンパク質UnaGのビリルビン結合依存的蛍光活性

熊谷安希子, 安藤亮子, 宮武秀行, GREIMEL Peter, 小林俊秀, 平林義雄, 下郡智美, 宮脇敦史

日本水産学会大会講演要旨集, 2013 54, Sep 19, 2013
Fluorescence imaging in the last two decades

Atsushi Miyawaki

Journal of Electron Microscopy, 62(1) 63-68, Feb, 2013
Nobel nonlinear optical microscopy for super-resolution deep imaging

ISOBE K., KAWANO H., TAKEDA T., SUDA A., KUMAGAI A., MIYAWAKI A., MIDORIKAWA K.

421 13-17, Dec 19, 2011
Visualization of G1 arrest of cell cycle induced by inhibition of PI-3 kinase in cancer cells: in vitro evaluations

Yumiko Mukai, Shingo Dan, Mutsumi Okamura, Yasumichi Inoue, Aki Hanyu, Asako Sakaue-Sawano, Takeshi Imamura, Atsushi Miyawaki, Takao Yamori

CANCER RESEARCH, 71, Apr, 2011
Novel Method 'Fucci' Elucidated the Cardiomyocyte Cell Cycle Dynamics in Various Life Stages

Hisayuki Hashimoto, Shinsuke Yuasa, Shugo Tohyama, Tomohisa Seki, Toru Egashira, Kojiro Yae, Dai Kusumoto, Masaki Kodaira, Fumiyuki Hattori, Naoto Muraoka …

CIRCULATION, 122(21), Nov, 2010
Measurement of two-photon excitation spectra of fluorescent proteins with Fourier-transform nonlinear spectroscopy

SUDA A., ISOBE K., HASHIMOTO H., KANNARI F., KAWANO H., MIZUNO H., MIYAWAKI A., MIDORIKAWA K.

404 49-53, Sep 13, 2010
Evaluating the performance of genetically encoded calcium indicators expressed in mammalian neurons

Yoshiyuki Yamada, Takayuki Michikawa, Mitsuhiro Hashimoto, Atsushi Miyawaki, Katsuhiko Mikoshiba

NEUROSCIENCE RESEARCH, 68 E334-E334, 2010
The role of polyunsaturated fatty acids in synaptogenesis

Hiroshi Hama, Atsushi Miyawaki

NEUROSCIENCE RESEARCH, 68 E43-E43, 2010
Myosin II modulates fusion pore opening period via cortical F-actin reorganization

Ryo Aoki, Tetsuya Kitaguchi, Atsushi Miyawaki, Takashi Tsuboi

JOURNAL OF PHYSIOLOGICAL SCIENCES, 60 S176-S176, 2010

Research Projects

The pursuit of both brightness and photostability in fluorescent protein technology

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, Jul, 2021 - Mar, 2026
Resoance Biology for Innovative Bioimaging

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2021
Accelerating international cooperative researches

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Nov, 2015 - Mar, 2021

Miyawaki Atsushi
The development of fundamental bioimaging technologies for comprehensive understanding

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2015 - Mar, 2020

Atsushi Miyawaki
Resonance Biology for Innovative Bioimaging

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2015 - Mar, 2020

Miyawaki Atsushi
Resonance Biology for Innovative Bioimaging

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Jun, 2015 - Mar, 2020
シナプス可塑性の基盤となるカルシウムシグナルの可視化

科学研究費助成事業, 特別研究員奨励費, 日本学術振興会, Jul, 2016 - Mar, 2019

宮脇敦史, 林康紀, ROSENDALE MORGANE
Development of fluorescent probe for visualization of neuronal connectivity and its application

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2017

KINOSHITA Nagatoki, MIYAWAKI Atsushi, SHIMOGORI Tomomi
Multi-dimensional fluorescent live imaging of cellular functions and molecular activities

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2010 - Mar, 2016

MICHIYUKI Matsuda, IMAMURA Takeshi, KIYOKAWA Etsuko, MIYAWAKI Atsushi, NEMOTO Tomomi, OKADA Takaharu, ISHII Masaru, FUKUHARA Shigetomo
Technological innovations for large-scale high-resolution 3D reconstruction of biological structures.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

MIYAWAKI Atsushi, HAMA Hiroshi, SHIMOGORI Tomomi, KUROKAWA Hiroshi, SAKAUE Asako, YOSHIHARA Yoshihiro, ANDO Ryoko
Crosstalk between chromosome modification and transcription factors in the differentiation of hematopoietic cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2010 - Mar, 2015

KITAMURA Toshio, SAKAUE Asako, YAMAMOTO Masayuki, ISHIKAWA Fumihiko, MIYAWAKI Atsushi
Visualizing biological functions at many different scales

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2010 - Mar, 2015

MIYAWAKI Atsushi, HAMA Hiroshi, SASAKI Kazuki, SHIMOZONO Satoshi, NIINO Yusuke, SAKAUE Asako, KAWANO Hiroyuki, FUKANO Takashi, ANDO Ryoko
Mechanisms and physiological roles of electro-chemical Coupling mediated by voltage-sensor domain proteins

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, May, 2009 - Mar, 2014

YASUSHI Okamura, SAKIMURA Kenji, YOSHIDA Manabu, NAKAGAWA Atsushi, FUJIWARA Yuichiro, TSUTSUI Hidekazu, OUKOUCHI Yoshifumi, MIYAWAKI Atsushi
Investigation for the bone clock development by bioluminescence and fluorescence imaging on brain and bone

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, 2011 - 2012

IIMURA Tadahiro, NUMANO Rika, YAMAGUCHI Akira, MIYAWAKI Atsushi
Activation of stem cells and neurogenesis in adult brains : their regulatory mechanism and visualization

Grants-in-Aid for Scientific Research, Grant-in-Aid for Creative Scientific Research, Japan Society for the Promotion of Science, 2005 - 2009

OKANO Hideyuki, YUZAKI Michisuke, MIYAWAKI Atsushi, YAMASHIMA Tetsumori, SAWAMOTO Kazunobu, ITOHARA Shigeyoshi
New approaches for imaging and controlling the molecules of life.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 2005 - 2009

MIYAWAKI Atsushi, MIZUNO Hideaki, SHIMOZONO Satoshi, FUKANO Takashi, TSUTSUI Hidekazu, NAGAI Takeharu, IBATA Keiji, MIYAUCHI Takayuki, VETRIVEL Lakshmanan
Photomodulatable fluorescent proteins

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2006 - 2007

ATSUSHI Miywaki, MIZUNO Hideaki, TSUTSUI Hidekazu
cameleon1分子イメージングによる局所的カルシウム動態の解析

科学研究費助成事業, 特定領域研究, 日本学術振興会, 1999 - 2003

宮脇敦史, 深野天, 濱裕, 水野秀昭, 河野弘幸, 筒井秀和, 永井健治
Development and application ofa novel genetic strategy for visualization offunctional neural pathways with WGA-GFP fusion transgene

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2000 - 2001

YOSHIHARA Yoshihiko, FUNAKI Atsuko, SAIO Michiko, MIYAWAKI Atsushi
Imaging of Synamics of Cellular Functions using GFP-based probes

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B)., Japan Society for the Promotion of Science, 1999 - 2000

MIYAWAKI Atsushi, MIZUNO Hideaki, HAMA Hiroshi

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