Kouji Hirota

Profile Information

Affiliation: Graduate School of Science, Graduate School of Science, Tokyo Metropolitan University

Degree: 博士（理学）(東京大学大学院理学系研究科生物化学専攻)

J-GLOBAL ID: 201201084500571295
researchmap Member ID: B000221291

External link: https://khirota9.wixsite.com/website

Research Interests

Research Areas

Research History

Apr, 2012 - Present

Graduate School of Science, Graduate School of Science, Tokyo Metropolitan University
May, 2010 - Mar, 2012

准教授, 京都大学医学(系)研究科(研究院)
Apr, 2008 - Apr, 2010

助教, 京都大学医学（系）研究科(研究院)
Apr, 2005 - Mar, 2008

基礎科学特別研究員, 理化学研究員
Apr, 2001 - Mar, 2005

協力研究員, 理化学研究所

Education

Apr, 1998 - Mar, 2001

Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo
Apr, 1996 - Mar, 1998

Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo
Apr, 1992 - Mar, 1996

Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo

Papers

102

The proofreading exonuclease of leading-strand DNA polymerase epsilon prevents replication fork collapse at broken template strands

Tasnim Ahmad, Ryotaro Kawasumi, Tomoya Taniguchi, Takuya Abe, Kazuhiro Terada, Masataka Tsuda, Naoto Shimizu, Toshiki Tsurimoto, Shunichi Takeda, Kouji Hirota

Nucleic Acids Research, Nov 8, 2023
PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion.

Ivan Psakhye, Ryotaro Kawasumi, Takuya Abe, Kouji Hirota, Dana Branzei

Nature structural & molecular biology, Aug 17, 2023
CTF18-RFC contributes to cellular tolerance against chain-terminating nucleoside analogs (CTNAs) in cooperation with proofreading exonuclease activity of DNA polymerase ε.

Mubasshir Washif, Tasnim Ahmad, Md Bayejid Hosen, Md Ratul Rahman, Tomoya Taniguchi, Hiromori Okubo, Kouji Hirota, Ryotaro Kawasumi

DNA repair, 127 103503-103503, Jul, 2023
Application of neural network-based image analysis to detect sister chromatid cohesion defects

Daiki Ikemoto, Tomoya Taniguchi, Kouji Hirota, Kiyoshi Nishikawa, Kan Okubo, Takuya Abe

Scientific Reports, 13(1), Feb 6, 2023
SPRTN and TDP1/TDP2 Independently Suppress 5-Aza-2′-deoxycytidine-Induced Genomic Instability in Human TK6 Cell Line

Toshiaki Nakano, Takahito Moriwaki, Masataka Tsuda, Misa Miyakawa, Yuto Hanaichi, Hiroyuki Sasanuma, Kouji Hirota, Masanobu Kawanishi, Hiroshi Ide, Keizo Tano …

Chemical Research in Toxicology, 35(11) 2059-2067, Oct 25, 2022 Peer-reviewed
<scp>XRCC1</scp> counteracts poly(ADP ribose)polymerase (PARP) poisons, olaparib and talazoparib, and a clinical alkylating agent, temozolomide, by promoting the removal of trapped <scp>PARP1</scp> from broken <scp>DNA</scp>

Kouji Hirota, Masato Ooka, Naoto Shimizu, Kousei Yamada, Masataka Tsuda, Mahmoud Abdelghany Ibrahim, Shintaro Yamada, Hiroyuki Sasanuma, Mitsuko Masutani, Shunichi Takeda

Genes to Cells, Mar, 2022
Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes.

Naushin L Hindul, Amarjot Jhita, Daiana G Oprea, Tasnim Alamgir Hussain, Oksana Gonchar, Miguel Angel Muro Campillo, Laura O'Regan, Masato T Kanemaki, Andrew M Fry, Kouji Hirota …

Biology open, 11(2), Feb 15, 2022
lncRNA transcription induces meiotic recombination through chromatin remodelling in fission yeast

Satoshi Senmatsu, Ryuta Asada, Arisa Oda, Charles S. Hoffman, Kunihiro Ohta, Kouji Hirota

Communications Biology, 4(1), Dec, 2021
Targeting chromosome trisomy for chromosome editing.

Takuya Abe, Yuya Suzuki, Teppei Ikeya, Kouji Hirota

Scientific reports, 11(1) 18054-18054, Sep 10, 2021
Vertebrate CTF18 and DDX11 essential function in cohesion is bypassed by preventing WAPL-mediated cohesin release

Ryotaro Kawasumi, Takuya Abe, Ivan Psakhye, Keiji Miyata, Kouji Hirota, Dana Branzei

Genes & Development, Sep 9, 2021
Follow-up genotoxicity assessment of Ames-positive/equivocal chemicals using the improved thymidine kinase gene mutation assay in DNA repair-deficient human TK6 cells.

Akira Sassa, Takayuki Fukuda, Akiko Ukai, Maki Nakamura, Ryosuke Sato, Sho Fujiwara, Kouji Hirota, Shunichi Takeda, Kei-Ichi Sugiyama, Masamitsu Honma …

Mutagenesis, Jul 3, 2021
XRCC1 prevents toxic PARP1 trapping during DNA base excision repair

Annie A. Demin, Kouji Hirota, Masataka Tsuda, Marek Adamowicz, Richard Hailstone, Jan Brazina, William Gittens, Ilona Kalasova, Zhengping Shao, Shan Zha …

Molecular Cell, Jun, 2021
Division of labor of Y-family polymerases in translesion-DNA synthesis for distinct types of DNA damage

Yuriko Inomata, Takuya Abe, Masataka Tsuda, Shunichi Takeda, Kouji Hirota

PLOS ONE, 16(6) e0252587-e0252587, Jun 1, 2021
Pold4, the fourth subunit of replicative polymerase δ, suppresses gene conversion in the immunoglobulin-variable gene in avian DT40 cells.

Kota Kojima, Masato Ooka, Takuya Abe, Kouji Hirota

DNA repair, 100 103056-103056, Apr, 2021
Topoisomerase activity is linked to altered nucleosome positioning and transcriptional regulation in the fission yeast fbp1 gene

Ryuta Asada, Satoshi Senmatsu, Ben Montpetit, Kouji Hirota

PLOS ONE, 15(11) e0242348-e0242348, Nov 12, 2020
Direct Determination of Pseudouridine in RNA by Mass Spectrometry Coupled with Stable Isotope Labeling.

Yuka Yamaki, Yuko Nobe, Masami Koike, Yoshio Yamauchi, Kouji Hirota, Nobuhiro Takahashi, Hiroshi Nakayama, Toshiaki Isobe, Masato Taoka

Analytical chemistry, 92(16) 11349-11356, Aug 18, 2020
Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross-links in chicken DT40 cells

Toshiaki Nakano, Mahmoud I. Shoulkamy, Masataka Tsuda, Hiroyuki Sasanuma, Kouji Hirota, Minoru Takata, Shin-ichiro Masunaga, Shunichi Takeda, Hiroshi Ide, Tadayoshi Bessho …

PLOS ONE, 15(6) e0234859-e0234859, Jun 26, 2020
Topoisomerase I-driven repair of UV-induced damage in NER-deficient cells.

Liton Kumar Saha, Mitsuo Wakasugi, Salma Akter, Rajendra Prasad, Samuel H Wilson, Naoto Shimizu, Hiroyuki Sasanuma, Shar-Yin Naomi Huang, Keli Agama, Yves Pommier …

Proceedings of the National Academy of Sciences of the United States of America, 117(25) 14412-14420, Jun 23, 2020 Peer-reviewed
UBC13-mediated ubiquitin signaling promotes removal of blocking adducts from DNA double-strand breaks

Remi Akagawa, Hai Thanh Trinh, Liton Kumar Saha, Masataka Tsuda, Kouji Hirota, Shintaro Yamada, Atsushi Shibata, Masato T. Kanemaki, Shinichiro Nakada, Shunichi Takeda …

iScience, in press, Mar, 2020 Peer-reviewed
Roles of lncRNA transcription as a novel regulator of chromosomal function

Satoshi Senmatsu, Kouji Hirota

Genes & Genetic Systems, 2020

Misc.

Activation induced deaminase(AID)のconditional knockoutによる抗体遺伝子再編成制御技術の開発

松井晋, 瀬尾秀宗, 村山晃歩, 黒澤恒平, 阿部拓也, 廣田耕志, 太田邦史

日本分子生物学会年会プログラム・要旨集(Web), 42nd, 2019
lncRNAによるストレス種特異的な遺伝子発現の制御

岡崎賢吾, 竹俣直道, 小田有沙, 山田貴富, ガリポンジョゼフィーヌ, 三好知一郎, 鈴木穣, 菅野純夫, HOFFMAN Charles S., 廣田耕志 …

日本分子生物学会年会プログラム・要旨集(Web), 39th, 2016
Ctf18-RFCのPCNA装着は非DNA合成モードのPolεによって促進される

藤澤遼, 大橋英治, 廣田耕志, 釣本敏樹

日本分子生物学会年会プログラム・要旨集(Web), 39th, 2016
Tup family corepressorとCCAAT-binding factorによるクロマチン構造変化の拮抗的な制御がmRNA転写開始点を決定する

浅田隆大, 竹俣直道, 太田邦史, 廣田耕志

日本分子生物学会年会プログラム・要旨集(Web), 37th, 2014
長鎖ノンコーディングRNAの転写はクロマチン制御因子の呼び込みをポジティブフィードバック的に促進する

竹俣直道, 廣田耕志, 山田貴富, 小田有沙, JOSEPHINE Galipon, 三好知一郎, HOFFMAN Charles, 太田邦史

日本分子生物学会年会プログラム・要旨集(Web), 36th, 2013
複製チェックポイントと染色体高次構造を仲介する新規因子による遺伝的組換え開始制御

伊藤将, 三好知一郎, 久郷和人, 山田真太郎, 山田真太郎, 古市真樹, 小田有沙, 小田有沙, 山田貴富, 廣田耕志 …

日本遺伝学会大会プログラム・予稿集, 84th, 2012
染色体高次構造とS期チェックポイントを減数分裂期組換えの開始と連係させるリエゾン因子

伊藤将, 三好知一郎, 三好知一郎, 久郷和人, 山田真太郎, 古市真樹, 小田有沙, 山田貴富, 廣田耕志, 廣田耕志 …

日本分子生物学会年会プログラム・要旨集(Web), 35th, 2012
ヒストン修飾酵素Gcn5およびSet1はlncRNAの転写と協調してグルコース飢餓応答に働く

竹俣直道, 廣田耕志, GALIPON Josephine, 小田有沙, 三好知一郎, 山田貴富, 太田邦史

日本分子生物学会年会プログラム・要旨集(Web), 35th, 2012
ゲノムの“ダークマター”長鎖ncRNAが制御する多彩な生命現象長鎖ncRNAによるクロマチン・転写活性化の制御

太田邦史, 太田邦史, 小田有沙, GALIPON Josephine, 竹俣直道, 三好知一郎, 廣田耕志

実験医学, 29(11), 2011
長鎖ノンコーディングRNAの転写によるヒストン修飾制御と遺伝発現制御

竹俣直道, 廣田耕志, GALIPON Josephine, 三好知一郎, HOFFMAN Charles, 太田邦史

日本分子生物学会年会プログラム・要旨集(Web), 34th, 2011
Creating cell models of familial Parkinson's disease using the chicken DT40 B cell line

Eiko Minakawa, Satoshi Horimoto, Kouji Hirota, Shunichi Takeda, Ryosuke Takahashi

NEUROSCIENCE RESEARCH, 71 E191-E191, 2011
グルコース飢餓時におけるmRNA型長鎖非翻訳RNA発現のゲノムワイド解析

小田有沙, 三好知一郎, 久郷和人, 廣田耕志, 太田邦史

生化学, 2010
ノンコーディングRNAの転写を介したクロマチン再編成・ヒストン修飾の制御と遺伝子活性化機構

竹俣直道, 廣田耕志, 廣田耕志, JOSEPHINE Galipon, 三好知一郎, 太田邦史

生化学, 2010
mlonRNA 仮説 : ノンコーディングRNA転写による新規クロマチン構造変化機構

廣田耕志, 太田邦史

化学と生物, 47(5) 296-298, May 1, 2009
Analyses of the polymorphic Dmc-1-M200V variant

J. Hikiba, K. Hirota, W. Kagawa, S. Ikawa, T. Kinebuchi, I. Sakane, Y. Takizaw, S. Yokoyama, B. Mandon-Pipin, A. Nicolas …

FEBS JOURNAL, 275 113-113, Jun, 2008
染色体増幅,組換え,修復の制御 4.減数分裂期の組換え開始制御

廣田耕志, 福田智行, 太田邦史

実験医学, 25(5) 732-738, Mar 15, 2007
Chromatin regulation at fission yeast CRE-related sequences

Kouji Hirota, Takehiko Shibata, Kunihiro Ohta

CELL STRUCTURE AND FUNCTION, 30 94-94, Jun, 2005
Analysis of cell oriented elongation mechanisms which is involved in sexial development

HIROTA Kouji, TANAKA Kayoko, YAMAMOTO Masayuki

21 554-554, Dec 1, 1998
SMN-GEMIN2 comple χ stimulates the RAD51-mediated recombination reactions

Takaku M, Tsujita T, Horikoshi N, Takizawa Y, Qing Y, Hirota K, Ikura M, Ikura T, Takeda S, Kurumizaka, H

第34 回日本分子生物学会年会 12 月15 日横浜 Peer-reviewed

Professional Memberships

Research Projects

相同的組換えでの相同並行三重鎖DNAの働き

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2022 - Mar, 2025

柴田武彦, 廣田耕志
International collaborations for revealing the synergistic genetic relationship in genome maintenance systems

Grants-in-Aid for Scientific Research, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Japan Society for the Promotion of Science, Oct, 2019 - Mar, 2024
Search for a pioneer polymerase complex that opens chromatin in non-coding RNA transcribed regions

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Japan Society for the Promotion of Science, Jul, 2021 - Mar, 2023
Role of proofreading exonuclease activity of replicative polymerase epsilon in replication fork slowing at DNA damage

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
Establishment of Novel Bioassays for in vivo Genotoxicity Prediction and Mechanism Characterization

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, May, 2016 - Mar, 2021

Takeda Shunichi
Analysis of the regulatory network of polymerases upon replication arrest

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

hirota kouji, ABE Takuya
Regulation of chromatin and genome higher order structure by non-coding RNAs

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2018
Analysis of Translesion synthesis (TLS) mechanism by novel assay method

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2018

Hirota Kouji, Julian Sale, Doherty Aidan, Branzei Dana, Scott Keeney, Hoffman Charles S., Pommier Yves
ノンコーディングRNA転写と共役したクロマチン構造変化の制御機構の解明

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2014 - Mar, 2016

廣田耕志
Analysis of genome maintenance system by the combination of proteomics and genetic approach

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2016

hirota kouji
Establishment of high-throughput screening of toxic chemical compounds.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (S), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2016

Takeda Shunichi, HIROTA Kouji, YAMADA Ryo, OKADA Tetsuya, SASANUMA Hiroyuki, SHIMIZU Hiroyasu, TAKAHASHI Ryosuke
複製ポリメラーゼδによる複製フォークの安定化機構の解明

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2012 - Mar, 2014

廣田耕志
Chromatin regulation by lncRNA

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), Japan Society for the Promotion of Science, Apr, 2009 - Mar, 2014

OHTA Kunihiro, YAMADA Takatomi, KUGOU Kazuto, HIROTA Kouji
Analysis of DNA repair pathway by the combination of proteomics andgenetic approach

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (A), Japan Society for the Promotion of Science, 2010 - 2012

HIROTA Kouji
ノンコーディングRNA転写に共役する新規クロマチン制御機構の解明

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, 2009 - 2010

廣田耕志
Method to enhance gene-targeting efficiency

Grants-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, 2008 - 2009

HIROTA Kouji
Interplay among recombination, replication, segregation, and chromatin structure.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Priority Areas, Japan Society for the Promotion of Science, 2005 - 2009

OHTA Kunihiro, HIROTA Kouji, FUKUDA Tomoyuki, BRANZEI Dana, YAMADA Takatomi
Chromatin regulation around M26/CRE DNA sequences

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2007 - 2008

OHTA Kunihiro, HIROTA Kouji, HIROTA Kouji, YAMADA Takatomi
Mechanisms of chromatin remodeling and recombination activation in fission yeast starvation and stress response

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2005 - 2006

OHTA Kunihiro, HIROTA Kouji
酵母染色体組換えにおける染色体機能装置の連携

科学研究費助成事業, 特定領域研究, 日本学術振興会, 1999 - 2003

太田邦史, 梅津桂子, 廣田耕志, 山田貴富, 水野健一

Profile Information

Research Interests

Research Areas

Research History

Education

Papers

Misc.

Professional Memberships

Research Projects

Menu

Coauthors