Kenji Mizutani

Profile Information

Affiliation: Yokohama City University

Degree: 博士(理学)(横浜市立大学)

Researcher number: 10525570
J-GLOBAL ID: 201601019789365047
researchmap Member ID: 7000017942

横浜市立大学大学院総合理学研究科博士後期課程修了(2007年)博士(理学)。ERATO岩田プロジェクト技術補助。インペリアルカレッジロンドン、リサーチアソシエート。京都大学大学院医学研究科産官学連携研究員(分子細胞情報学)。東京理科大学基礎工学部ポストドクトラル研究員。千葉大学大学院理学研究科(化学コース)特任研究員、同特任助教を経て、2016年横浜市立大学生命医科学研究科助教(構造創薬科学)。

Research Interests

Research Areas

Committee Memberships

Apr, 2017 - Mar, 2019

PF外部編集委員, PF news 編集委員会

Papers

Diselenide-bond replacement of the external disulfide bond of insulin increases its oligomerization leading to sustained activity.

Kenta Arai, Masaki Okumura, Young-Ho Lee, Hidekazu Katayama, Kenji Mizutani, Yuxi Lin, Sam-Yong Park, Kaichiro Sawada, Masao Toyoda, Hironobu Hojo …

Communications chemistry, 6(1) 258-258, Nov 21, 2023
Structural basis of CXC chemokine receptor 1 ligand binding and activation.

Naito Ishimoto, Jae-Hyun Park, Kouki Kawakami, Michiko Tajiri, Kenji Mizutani, Satoko Akashi, Jeremy R H Tame, Asuka Inoue, Sam-Yong Park

Nature communications, 14(1) 4107-4107, Jul 11, 2023 Peer-reviewed
E. coli production of a multi-disulfide bonded SARS-CoV-2 Omicron BA.5 RBD exhibiting native-like biochemical and biophysical properties.

Rawiwan Wongnak, Subbaian Brindha, Takahiro Yoshizue, Sawaros Onchaiya, Kenji Mizutani, Yutaka Kuroda

Biophysics and physicobiology, 20(4) e200036, 2023
Structural insights into the HBV receptor and bile acid transporter NTCP.

Jae-Hyun Park, Masashi Iwamoto, Ji-Hye Yun, Tomomi Uchikubo-Kamo, Donghwan Son, Zeyu Jin, Hisashi Yoshida, Mio Ohki, Naito Ishimoto, Kenji Mizutani …

Nature, 606(7916) 1027-1031, May 17, 2022 Peer-reviewed
Reverse Engineering Analysis of the High-Temperature Reversible Oligomerization and Amyloidogenicity of PSD95-PDZ3.

Sawaros Onchaiya, Tomonori Saotome, Kenji Mizutani, Jose C Martinez, Jeremy R H Tame, Shun-Ichi Kidokoro, Yutaka Kuroda

Molecules (Basel, Switzerland), 27(9), Apr 28, 2022 Peer-reviewed
The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata

Kenichi Kamata, Kenji Mizutani, Katsuya Takahashi, Roberta Marchetti, Alba Silipo, Christine Addy, Sam-Yong Park, Yuki Fujii, Hideaki Fujita, Tsuyoshi Konuma …

Scientific Reports, 10(1), Dec, 2020 Peer-reviewed
Domain architecture divergence leads to functional divergence in binding and catalytic domains of bacterial and fungal cellobiohydrolases.

Akihiko Nakamura, Daiki Ishiwata, Akasit Visootsat, Taku Uchiyama, Kenji Mizutani, Satoshi Kaneko, Takeshi Murata, Kiyohiko Igarashi, Ryota Iino

The Journal of biological chemistry, Aug 18, 2020 Peer-reviewed
A Coil-to-Helix Transition Serves as a Binding Motif for hSNF5 and BAF155 Interaction.

Jeongmin Han, Iktae Kim, Jae-Hyun Park, Ji-Hye Yun, Keehyoung Joo, Taehee Kim, Gye-Young Park, Kyoung-Seok Ryu, Yoon-Joo Ko, Kenji Mizutani …

International journal of molecular sciences, 21(7), Apr 1, 2020 Peer-reviewed
Metastable asymmetrical structure of a shaftless V1 motor.

Shintaro Maruyama, Kano Suzuki, Motonori Imamura, Hikaru Sasaki, Hideyuki Matsunami, Kenji Mizutani, Yasuko Saito, Fabiana L Imai, Yoshiko Ishizuka-Katsura, Tomomi Kimura-Someya …

Science advances, 5(1) eaau8149-eaau8149, Jan, 2019 Peer-reviewed
Crystal structures of the ATP-binding and ADP-release dwells of the V1 rotary motor.

Kano Suzuki, Kenji Mizutani, Shintaro Maruyama, Kazumi Shimono, Fabiana L Imai, Eiro Muneyuki, Yoshimi Kakinuma, Yoshiko Ishizuka-Katsura, Mikako Shirouzu, Shigeyuki Yokoyama …

Nature communications, 7(13235) 13235-13235, Oct 27, 2016 Peer-reviewed
X-ray Crystallographic Structure of Thermophilic Rhodopsin: IMPLICATIONS FOR HIGH THERMAL STABILITY AND OPTOGENETIC FUNCTION.

Takashi Tsukamoto, Kenji Mizutani, Taisuke Hasegawa, Megumi Takahashi, Naoya Honda, Naoki Hashimoto, Kazumi Shimono, Keitaro Yamashita, Masaki Yamamoto, Seiji Miyauchi …

The Journal of biological chemistry, 291(23) 12223-32, Jun 3, 2016 Peer-reviewed
Binding interactions of the peripheral stalk subunit isoforms from human V-ATPase

Suhaila Rahman, Ichiro Yamato, Shinya Saijo, Kenji Mizutani, Yuuki Takamuku, Yoshiko Ishizuka-Katsura, Noboru Ohsawa, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama …

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(5) 878-890, May, 2016 Peer-reviewed
Crystal Structure of phosphate-bound V-1-ATPase of Enterococcus hirae

Kano Suzuki, Kenji Mizutani, Yoshiko Ishizuka-Katsura, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Takeshi Murata

PROTEIN SCIENCE, 23 229-229, Jul, 2014 Peer-reviewed
1P009 Crystal Structure of ADP-Bound A3B3 Complex of Enterococcus hirae V-ATPase(Protein: Structure,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Kazuya Nakamoto, Kenji Mizutani, Kano Suzuki, Yoshiko Ishizuka-Katsura, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Takeshi Murata

Seibutsu Butsuri, 54(1) S142, 2014
Rotation mechanism of Enterococcus hirae V1-ATPase based on asymmetric crystal structures.

Satoshi Arai, Shinya Saijo, Kano Suzuki, Kenji Mizutani, Yoshimi Kakinuma, Yoshiko Ishizuka-Katsura, Noboru Ohsawa, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama …

Nature, 493(7434) 703-7, Jan 31, 2013 Peer-reviewed
1P173 Purification and Crystallization of A subunit from Enterococcus hirae V-ATPase(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Saito Aki, Saito Yasuko, Saijo Shinya, Yamamoto Misaki, Kakinuma Yoshimi, Mizutani Kenji, Murata Takeshi, Yamato Ichiro

Seibutsu Butsuri, 53(1) S134, 2013
1P172 Expression of Enterococcus hirae V-ATPase in E. coli BL21 (DE3)(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Matsudo Shohei, Rahman Suhaila, Saijo Shinya, Yamamoto Misaki, Kakinuma Yoshimi, Mizutani Kenji, Murata Takeshi, Yamato Ichiro

Seibutsu Butsuri, 53(1) S134, 2013
Biochemical and biophysical properties of interactions between subunits of the peripheral stalk region of human V-ATPase.

Suhaila Rahman, Ichiro Yamato, Shinya Saijo, Kenji Mizutani, Yoshiko Ishizuka-Katsura, Noboru Ohsawa, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, So Iwata …

PloS one, 8(2) e55704, 2013 Peer-reviewed
Loose binding of the DF axis with the A3B3 complex stimulates the initial activity of Enterococcus hirae V1-ATPase.

Md Jahangir Alam, Satoshi Arai, Shinya Saijo, Kano Suzuki, Kenji Mizutani, Yoshiko Ishizuka-Katsura, Noboru Ohsawa, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama …

PloS one, 8(9) e74291-e74291, 2013 Peer-reviewed
Mutant LV(476-7)AA of A-subunit of Enterococcus hirae V1-ATPase: High affinity of A3B3 complex to DF axis and low ATPase activity.

Jahangir Alam, Ichiro Yamato, Satoshi Arai, Shinya Saijo, Kenji Mizutani, Yoshiko Ishizuka-Katsura, Noboru Ohsawa, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama …

SpringerPlus, 2(689) 689-689, 2013 Peer-reviewed

Misc.

バクテリア及びカビ由来GH6セロビオヒドロラーゼのドメイン機能の違い

中村彰彦, 石渡大貴, VISOOTSAT Akasit, 内山拓, 水谷健二, 金子哲, 村田武士, 五十嵐圭日子, 飯野亮太, 飯野亮太

応用糖質科学, 10(4), 2020
asialo-GM1に特異的に結合する二枚貝由来レクチンSeviLの構造解析

鎌田健一, 高橋捷也, 水谷健二, 藤井佑樹, 藤田英明, 小沼剛, 池上貴久, 大関泰裕, TAME Jeremy R.H.

日本糖質学会年会要旨集, 38th, 2019
N末端側βバレルドメインのクラウン構造が非対称性を生み,協動性を付与する

丸山慎太郎, 鈴木花野, 今村元紀, 佐々木輝, 松波秀行, 水谷健二, 水谷健二, 齋藤靖子, 今井ファビアナ・リカ, 石塚(桂)芳子 …

日本蛋白質科学会年会プログラム・要旨集, 18th 49, May 23, 2018
微生物型ロドプシンTRのX線結晶構造解析

水谷健二, 橋本直記, 塚本卓, 須藤雄気, 村田武士

KEK Progress Report (Web), (2016-8) ROMBUNNO.242 (WEB ONLY), Jan, 2017
Towards structure-based design of nobel V-ATPase inhibitors

水谷健二, 岩田想, 村田武士

化学工業, 62(2) 148-152, Feb, 2011
3P173 Subunit arrangement and assembly of peripheral stalk of the V-type Na^+-ATPase from Enterococcus hirae(Molecular motors,Oral Presentations)

Yamamoto Misaki, Unzai Satoru, Mizutani Kenji, Suno-Ikeda Chiyo, Yabuki-Miyata Yukako, Yamato Ichiro, Kakinuma Yoshimi, Ohabyashi Naomi, Terada Takaho, Toyama Mitsutoshi …

Biophysics, 47(1) S246, Nov 20, 2007

Books and Other Publications

骨研究最前線 : 代謝・疾病のメカニズムから再生医療・創薬・リハビリ機器・機能性食品開発まで

水谷健二、村田武士 (Role: Contributor, 骨粗しょう症に関与する分子モーターの回転メカニズムと創薬への取組み)

エヌ・ティー・エス, Oct, 2013 (ISBN: 9784864690782)
膜タンパク質構造研究

水谷健二, 山本三沙岐, 村田武士 (Role: Joint author, V-ATPaseの分子メカニズム)

化学同人, Oct, 2013

Research Projects

Structural study of infection molecular mechanism of the mouse norovirus.

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Mizutani Kenji
Molecular mechanism of the structural formation and functional regulation of the V1 rotary motor

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

Murata Takeshi, MIZUTANI Kenji, YAMATO Ichiro
Structural basis of ATP hydrolysis mechanism of V1-ATPase motor

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2014

MURATA Takeshi, YAMATO Ichiro, SAIJO Shinya, MIZUTANI Kenji

Industrial Property Rights

特許第6533324号 HEAT STABILIZATION MUTANT PREDICTOR, HEAT STABILIZATION MUTANT PREDICTION METHOD, AND PROGRAM

Media Coverage

横浜市大・国立感染症研究所など、B型肝炎ウイルスの受容体「胆汁酸輸送体」の立体構造を解明

日本経済新聞社, 日本経済新聞, https://www.nikkei.com/article/DGXZRSP632430_X10C22A5000000/, May 18, 2022 Newspaper, magazine

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