Naoki Okumura

J-GLOBAL         Last updated: Jul 11, 2016 at 16:01
 
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Name
Naoki Okumura

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I have been developing both pharmaceutical and tissue engineering treatment for corneal endothelial dysfunction. The corneal endothelium is critical for maintaining homeostatic corneal transparency, which it accomplishes by pump and barrier functions. When corneal endothelial cell density decreases to a critical level, the cornea exhibits haziness, with edema, due to decompensation of the corneal endothelial function.

We showed that a Rho-associated kinase (ROCK) inhibitor can be useful in treating corneal endothelial dysfunction. In 2009, we demonstrated that the selective ROCK inhibitor, Y-27632, showed unique effects on cultured CECs: 1) promotion of proliferation; 2) enhancement of cell adhesion; and 3) suppression of apoptosis. Our subsequent studies revealed that administration of ROCK inhibitors in the form of eye drops promotes CEC proliferation in rabbit and monkey in vivo models. In addition to animal experiments, we have started a clinical trial of ROCK inhibitor eye drops in combination for treatment of human corneal endothelial dysfunction patients.

We also demonstrated that in rabbit and monkey corneal endothelial dysfunction models, corneal transparency was restored via the injection of cultivated CECs with the selective ROCK inhibitor into the anterior chamber. One technical obstacle that currently exists is the difficulty of the in vitro expansion of CECs, as they exhibit fibroblastic phenotypes with loss of pump and barrier functions while under the culture conditions. However, we successfully established a CEC culture protocol for clinical use. This protocol enables the efficient in vitro expansion of CECs and may ultimately provide clinicians with a new therapeutic modality in regenerative medicine. Of note, our team has started the first in man clinical trial of cultivated CEC-injection therapy in Japan.

Research Areas

 
 

Academic & Professional Experience

 
Apr 2015
 - 
Today
Associate Professor, Department of Biomedical Engineering, Doshisha university
 
Dec 2011
 - 
Today
Visiting associate professor, Kyoto Prefectural University of Medicine
 
Apr 2011
 - 
Mar 2015
Assistant professor, Doshisha university
 
Apr 2010
 - 
Mar 2011
Assistant professor, Kyoto Prefectural University of Medicine
 
May 2006
 - 
Mar 2010
PhD course student, Kyoto Prefectural University of Medicine
 

Education

 
Apr 2006
 - 
Mar 2010
Kyoto Prefectural University of Medicine PhD course
 
Apr 1995
 - 
Mar 2001
Kyoto Prefectural University of Medicine
 

Awards & Honors

 
2011
“ROCK Inhibitor Regulates the G1-S Progression of Corneal Endothelial Cells”, Association for Research in Vision and Ophthalmology (ARVO) Kowa Travel Grant
 
2010
"The Effect of a ROCK Inhibitor on Corneal Endothelial Cell Migration in an In Vitro Wound-Healing Model", Association for Research in Vision and Ophthalmology (ARVO) International Travel Grant
 
2010
"Therapeutic Potential of a Rho Kinase (ROCK) Inhibitor for the Treatment of Corneal Endothelial Dysfunction", Gordon Research Conference Biology & Pathobiology Of The Cornea Travel Grant
 
2009
"A ROCK Inhibitor Enhances Corneal Endothelial Wound Healing in Both an In vitro and in vivo Model", Association for Research in Vision and Ophthalmology (ARVO) International Travel Grant
 

Published Papers

 
Rho kinase inhibitor enables cell-based therapy for corneal endothelial dysfunction. Sci Rep.2016:6:26113.
Okumura N, Sakamoto Y, Fujii K, Kitano J,Nakano S,Tsujimoto Y,Nakamura S,Ueno M,Hagiya M,Hamuro J,Matsuyama A,Suzuki S,Shiina T,Kinoshita S,Koizumi N
   May 2016   [Refereed]
Effect of the Rho-Associated Kinase Inhibitor Eye Drop (Ripasudil) on Corneal Endothelial Wound Healing.Invest Ophthalmol Vis Sci.57(3):1284-1292,2016.
Okumura N,Okazaki Y,Inoue R,Kakutani K,Nakano S,Kinoshita S,Koizumi N
   Mar 2016   [Refereed]
Rho-Associated Kinase Inhibitor Eye Drop (Ripasudil) Transiently Alters the Morphology of Corneal Endothelial Cells.Invest Ophthalmol Vis Sci.56(12):7560-7567,2015.
Okumura N,Okazaki Y,Inoue R,Nakano S,Fullwood NJ,Kinoshita S,Koizumi N
   Nov 2015   [Refereed]
Density-gradient centrifugation enables the purification of cultured corneal endothelial cells for cell therapy by eliminating senescent cells.Sci Rep.Oct 7:5:15005,doi: 10.1038/srep15005,2015.
Okumura N,Kusakabe A,Hirano H,Inoue R,Okazaki Y,Nakano S,Kinoshita S,Koizumi N
   Oct 2015   [Refereed]
Effect of the Rho Kinase Inhibitor Y-27632 on Wound Healing of the Corneal Endothelium.Invest Ophthalmol Vis Sci.56(10):6067-6074,2015.
Okumura N,Inoue R,Okazaki Y,Nakano S,Nakagawa H,Kinoshita S,Koizumi N
   Sep 2015   [Refereed]
Morphological Changes of Human Corneal Endothelial Cells after Rho-Associated Kinase Inhibitor Eye Drop (Ripasudil) Administration.A Prospective Open-Label Clinical Study.PLos One.10(9):e0136802.doi: 10.1371,2015.
Nakagawa H,Koizumi N,Okumura N,Suganami H,Kinoshita S
   2015   [Refereed]
Involvement of ZEB1 and Snail1 in excessive production of extracellular matrix in the Fuchs endothelial corneal dystrophy.Laboratory Investigation.95,1291-1304,2015.
Okumura N,Minamiyama R,Ho L,Kay EP,Kawasaki S,Tourtas T,Schlötzer-Schrehardt U,Kruse F,Young RD,Quantock AJ,Kinoshita S,Koizumi N
   2015   [Refereed]
Trinucleotide Repeat Expansion in the TCF4 Gene in Fuchs’ Endothelial Corneal Dystrophy in Japanese.Invest Ophthalmol Vis Sci.56(8):4865-4869,2015 (co-first authors)
Nakano M,Okumura N,Nakagawa H,Koizumi N,Ikeda Y,Ueno M,Yoshii K,Adachi H,Aleff R,Butz M,Highsmith E,Tashiro K,Wieben E,Kinoshita S,Baratz K
   2015   [Refereed]
Laminin-511 and 521 enable efficient in vitro expansion of human corneal endothelial cells.Invest Ophthalmol Vis Sci.56(5):2933-2942,2015.
Okumura N,Kakutani K,Numata R,Nakahara M,Schlötzer-Schrehardt U,Kruse K,Kinoshita S,Koizumi N.
   2015   [Refereed]
New therapeutic modality for corneal endothelial disease using Rho-associated kinase inhibitor eye drops.Cornea.Suppl 11:S25-31,2014.
Koizumi N,Okumura N,Ueno M,Kinoshita S
   2014   [Refereed]
Cell-based approach for treatment of corneal endothelial dysfunction.Cornea.Suppl 11:S37-41,2014.
Okumura N,Kinoshita S,Koizumi N
   2014   [Refereed]
Cell surface markers of functional phenotypic corneal endothelial cells.Invest Ophthalmol Vis Sci.55(11):7610-7618,2014.
Okumura N,Hirano H,Numata R,Nakahara M,Ueno M,Hamuro J,Kinoshita S,Koizumi N
   2014   [Refereed]
Derivation of Mesenchymal Stromal Cells from Pluripotent Stem Cells through a Neural Crest Lineage using Small Molecule Compounds with Defined Media.PLoS One.9(12):e112291,2014.
Fukuta M,Nakai Y,Kirino K,Nakagawa M,Sekiguchi K,Nagata S,Matsumoto Y,Yamamoto T,Umeda K,Heike T,Okumura N,Koizumi N,Sato T,Nakahata T,Saito M,Otsuka T,Kinoshita S,Ueno M,Ikeya M,Toguchida J
   2014   [Refereed]
R-spondin1 Regulates Cell Proliferation of Corneal Endothelial Cells via the Wnt3a/β-catenin Pathway.Invest Ophthalmol Vis Sci.55(10):6861-6869,2014.
Okumura N,Nakamura T,Kay EP,Nakahara M,Kinoshita S,Koizumi N
   2014   [Refereed]
Synthetic Molecules that Protect Cells from Anoikis and Their Use in Cell Transplantation.Angewandte Chemie.53(42):11208-11213,2014.
Frisco H L,Watanabe M,Okumura N,Kusamori K,Takemoto N,Takaya J,Sato S,Yamazoe S,Takakura Y,Kinoshita S,Nishikawa M,Koizumi N,Uesugi M
   2014   [Refereed]
Cultivation of Corneal Endothelial Cells on a Pericellular Matrix Prepared from Human Decidua-Derived Mesenchymal Cells.PLoS One.9(2):e88169,2014.
Numata R,Okumura N,Nakahara M,Ueno M,Kinoshita S,Kanematsu D,Kanemura Y,Sasai Y,Koizumi N
   2014   [Refereed]
Involvement of cyclin D and p27 in cell proliferation mediated by ROCK inhibitors Y-27632 and Y-39983 during corneal endothelium wound healing.Invest Ophthalmol Vis Sci.55(1):318-329,2014.
Okumura N,Nakano S,Kay EP,Numata R,Ota A,Sowa Y,Sakai T,Ueno M,Kinoshita S,Koizumi N
   2014   [Refereed]
Human Corneal Endothelium Regeneration.Effect of ROCK Inhibitor.Invest Ophthalmol Vis Sci.19:54(8):5594-5595,2013.
Koizumi N,Okumura N,Kinoshita S.
   2013   [Refereed]
Ferret animal model of corneal endothelial dysfunction for evaluation of drug effect on corneal endothelial wound healing.Anim Eye Res.32:15-21,2013.
Sakamoto Y,Okumura N,Koizumi N,Numata R,Kitano J,Yamamoto M,Komada T,Hoshi N
   2013   [Refereed]
Corneal endothelial expansion promoted by human bone marrow mesenchymal stem cell-derived conditioned medium.PLoS One.23:8(7):e69009,2013.
Nakahara M,Okumura N,Kay EP,Hagiya M,Imagawa K,Hosoda Y,Kinoshita S,Koizumi N
   2013   [Refereed]

Misc

 
Rhoキナーゼ阻害薬(リパスジル)の角膜内皮創傷治癒に対する影響.日本眼科学会雑誌 120(7): 503,2016.6.
奥村直毅,岡崎友吾,井上亮太,角谷和哉、中野新一郎,木下茂,小泉範子
   Jun 2016

Conference Activities & Talks

 
The effect of p38 mitogen-activated protein kinase inhibitor on cell density and phenotype of cultivated human corneal endothelial cells.The Association for Research in Vision and Ophthalmology 2016,Seattle,USA,2016.5.4.(ポスター)
Hongo A,Okumura N,Nakahara M,Koizumi N
May 2016   
Effect of the rho-associated kinase inhibitor eye drop ripasudil on corneal endothelial wound healing in a rabbit corneal endothelial damage model. The Association for Research in Vision and Ophthalmology 2016, Seattle, USA, 2016.5.4.
Shimada T,Okumura N,Inoue R,Okazaki Y,Nakano S,Kinoshita S,Koizumi N
May 2016   
p38 mitogen-activated protein kinase inhibitor suppresses apoptosis in a Fuchs endothelial corneal dystrophy cellular model.The Association for Research in Vision and Ophthalmology 2016,Seattle,USA,2016.5.4.(ポスター)
Onishi T,Okumura N,Kusakabe A,Kitahara M,Hashimoto K,Nakahara M,Ueda E,Tourtas T,Schlötzer-Schrehardt U,Kruse F,Koizumi N
May 2016   
Feasibility of cell preservation as a form of cell suspension for a cell-based therapy in a rabbit corneal endothelial dysfunction model.The Association for Research in Vision and Ophthalmology 2016,Seattle,USA,2016.5.4.(ポスター)
Matsumoto D,Okumura N,Inoue R,Okazaki Y,Kinoshita S,Koizumi N
May 2016   
Effect of rho-associated kinase inhibitor eye drop (ripasudil) on morphology of corneal endothelial cells in humans and rabbits.The Association for Research in Vision and Ophthalmology 2016,Seattle,USA,2016.5.4.(ポスター)
Koizumi N,Okumura N,Okazaki Y,Inoue R,Nakano S,Suganami H,Fullwood N,Nakagawa H,Kinoshita S
May 2016   
Trinucleotide repeat expansion and TCF4 gene expression in Fuchs endothelial corneal dystrophy.The Association for Research in Vision and Ophthalmology 2016,Seattle,USA,2016.5.3.
Ogata K,Okumura N,Hayashi R,Nakahara M,Nakano M,Tashiro K,Kinoshita S,Schlötzer-Schrehardt U,Tourtas T,Kruse F,Koizumi N
May 2016   
Unfolded protein accumulation induced endoplasmic reticulum stress of corneal endothelial cells in Fuchs endothelial corneal dystrophy.The Association for Research in Vision and Ophthalmology 2016,Seattle,USA,2016.5.3.
Okumura N,Hashimoto K,Kitahara M,Nakahara M,Kinoshita S,Tourtas T,Schlötzer-Schrehardt U,Kruse F,Koizumi N
May 2016   
Cultivaled Corneal Endothelial Cell Transplantation.The 31st Asia-Pacific Academy of Ophthalmology Congress (APAO 2016) Taipei,Taiwan,2016.3.24
Naoki Okumura
Mar 2016   
Endotheial Cell Transplantation and Regeneration. DOG congress 2015, Berlin, Germany, 2015.10.4.
Naoki Okumura
Oct 2015   
How to make cornea endothelial cells. 6th EuCornea Congress, XXXIII Congress of the ESCRS, Barcelona, Spain, 2015.9.5.
Naoki Okumura
Sep 2015   
Development of Cell Therapy for the Treatment of Corneal Endothelial Dysfunction. Chulalongkorn Eye Center, Thailand.2015.8.19.
Naoki Okumura
Aug 2015   
An allogeneic transplantation trial of cynomolgus monkey ipsc-derived corneal endothelial-like cells.The International Society for Stem Cell Research.Stockholm,Sweden,2015.6.24.
Yoshida S,Hatou S,Okumura N,Koizumi N,Kinoshita S,Tsujikawa M,Hayashi R,Nishida K,Tsubota K,Shimmura S
Jun 2015   
Clinical research of cultured corneal endothelial transplantation. The Association for Research in Vision and Ophthalmology 2015, Colorado, USA, 2015.5.4.
Naoki Okumura
May 2015   
Existence of inflammatory cells on donor corneal endothelium in a Descemet’s stripping automated endothelial keratoplasty rabbit model.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.6.(ポスター)
Koudouna E,Okumura N,Nakano S,Inoue R,Fullwood N,Okazaki S,Kinoshita S,Koizumi N
May 2015   
Rho-associated protein kinase inhibitor suppresses corneal endothelial cell apoptosis by suppressing cell contraction via inhibiting myosin light chain phosphorylation.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.5.
Fujii K,Okumura N,Odajima A,Ueno M,Kinoshita S,Koizumi N
May 2015   
Morphological and functional evaluation of mitochondria in a Fuchs’ endothelial corneal dystrophy cell model.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Kitahara M,Okumura N,Schlotzer-Schrehardt U,Kruse FE,Young RD,Quantock AJ,Kinoshita S,Koizumi N
May 2015   
Effect of the cell density of cultivated corneal endothelial cells on tissue engineering for the treatment of corneal endothelial dysfunction.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Okumura N,Nakano S,Kusakabe A,Inoue R,Okazaki Y,Kakutani K,Kinoshita S,Koizumi N
May 2015   
Feasibility of Descemet’s membrane removal during cultivated corneal endothelial cell injection in rabbit and monkey models.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Koizumi N,Okumura N,Nakano S,Kitano J,Kinoshita S
May 2015   
Purification of high cell density cultured corneal endothelial cell by density-gradient centrifugation.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Kusakabe A,Okumura N,Hirano H,Koizumi N,Kinoshita S
May 2015   
Investigation of the donor tissue information on the phenotypes of cultivated human corneal endothelium.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Tanaka H,Ueno M,Toda M,Hamuro J,Yoshii K,Koizumi N,Okumura N,Kinoshita S,Montoya M,Iliakis B
May 2015   
The feasibility of recombinant human laminin-511 E8 fragments for human corneal endothelial cell cultivation.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Kakutani K,Okumura N,Schlotzer-Schrehardt U,Kruse FE,Kinoshita S,Koizumi N
May 2015   
The different binding properties of cultured human corneal endothelial cell subpopulations to Descemet’s membrane components.The Association for Research in Vision and Ophthalmology 2015.Colorado,USA,2015.5.3.(ポスター)
Toda M,Ueno M,Hiraga A,Asada K,Nakamura T,Hagiya M,Okumura N,Koizumi N,Hamuro J,Kinoshita S
May 2015   
角膜内皮障害に対する新しい治療法の開発〜再生医療と薬物療法〜. 京滋眼科臨床懇話会, 京都, 2015.4.25.
Naoki Okumura
Apr 2015   
R-spondin1の角膜内皮細胞増殖に対する影響.角膜カンファランス2015第39回日本角膜学会総会・第31回日本角膜移植学会,高知 ,2015.2.12.
Feb 2015   
ROCK-inhibiter Suppressed Apoptosis of Corneal Endothelial Cells by Inhibiting Membrane Blebbing.The Association for Research in Vision and Ophthalmology 2014.Florida,USA,2014.5.6.
Fujii K,Okumura N,Kay EP,Nakahara M,Odajima A,Ueno M,Kinoshita S,Koizumi N
2014   
ROCK Inhibitor Suppresses Apoptosis of Corneal Endothelial Cells by Inhibiting MLC phosphorylation. 4th Biennial Scientific Meeting Asia Cornea Society. Taipei, Taiwan, 2014.12.11.
Naoki Okumura
2014   
Cultivated corneal endothelial cell injection for the treatment of bullous keratopathy. Seminar in Kyungpook National University Hospital, 2014.9.27
Naoki Okumura
Sep 2014   
Regenerative medicine for the treatment of corneal endothelial dysfunction. (Keynote lecture), TERMIS-AP 2014, Daegu, Korea, 2014.9.26
Naoki Okumura
2014   
Development of cell therapy for treating corneal endothelial dysfunction. Seminar in the Catholic University of Korea. Seoul, Korea, 2014.9.23
Naoki Okumura
2014   
Involvement of transforming growth factor beta in extracellular matrix deposition and endoplasmic reticulum stress in FUCHS' corneal dystrophy.The International Society for Eye Research XXI Biennial Meeting,San Francisco,California,USA,2014.7.22.
Okumura N,Minamiyama R,Kay EP,Ho L,Kawasaki S,Young R,Quantock A,Schlotzer-Schrehardt U,Kruse F,Kinoshita S,Koizumi N
2014   
Modulating Cell Adhesion Property Enables Cell-based Therapy for Corneal Endothelial Dysfunction.The Association for Research in Vision and Ophthalmology 2014.Florida,USA,2014.5.7.
Okumura N
2014   
The efficiency of laminin-511 and laminin-521 as extracellular matrix for human corneal endothelial cell culture.The Association for Research in Vision and Ophthalmology 2014.Florida,USA,2014.5.5.(ポスター)
Kakutani K,Okumura N,Numata R,Schlotzer-Schrehardt U,Kruse F,Kinoshita S,Koizumi N
2014   
The effect of cell density on the adhesion and proliferation properties of cultivated corneal endothelial cells.The Association for Research in Vision and Ophthalmology 2014.Florida,USA,2014.5.5.(ポスター)
Kusakabe A,Okumura N,Hirano H,Kinoshita S,Koizumi N
2014   
The involvement of transforming growth factor beta on excessive extracellular matrix production of corneal endothelial cells in Fuchs’ endothelial corneal dystrophy.The Association for Research in Vision and Ophthalmology 2014.Florida,USA,2014.5.6.
Koizumi N,Okumura N,Ho L,Kay EP,Kawasaki S,Tourtas T,Schlotzer-Schrehardt U,Kruse F,Kinoshita S
2014   
The involvement of transforming growth factor beta in endoplasmic reticulum stress of corneal endothelial cells in Fuchs’ endothelial corneal dystrophy.The Association for Research in Vision and Ophthalmology 2014.Florida,USA,2014.5.5.(ポスター)
Okumura N,Minamiyama R,Kay EP,Kawasaki S,Young R,Quantock A,Schlotzer-Schrehardt U,Friedrich EK,Kinoshita S,Koizumi N
2014   
Cell-injection therapy for the treatment of corneal endothelial dysfunction.第118回日本眼科学会総会,東京2014.4.3.(WOC2014 Otsuka morning seminar)
Okumura N
Apr 2014   
Cell-Based Approach for the Treatment of Corneal Endothelial Dysfunction.Gordon Research Conference.California,USA,2014.2.17.
Okumura N
2014   
The Effect of EMT-Related Genes on the Regulation of Excessive Production of Extracellular Matrix in a Cellular Model of Fuchs’ Corneal Dystrophy.Gordon Research Conference.California,USA,2014.2.17.
Okumura N,Leona Ho,EunDuck P.Kay,Kawasaki S,Theofilos Tourtas,Schlotzer-Schrehardt U,Kruse FE,Kinoshita S,Koizumi N
2014   
霊長類水疱性角膜症モデルを用いた培養角膜内皮細胞移植の有用性および安全性評価.第13回日本再生医療学会総会, 京都, 2014.3.6.
小泉範子、奥村直毅、椎名隆、鈴木進悟、中村紳一朗、上野盛夫、羽室淳爾、松山晃文、木下茂
Mar 2014   
Rhoキナーゼ阻害剤の角膜内皮細胞に対するアポトーシス抑制効果の検討.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山, 2013.2.14.
小田嶋愛、奥村直毅、Eunduck P Kay、陳文、上野盛夫、木下茂、小泉範子
2013   
Effect of SAHA on Fibrotic Change in Primate Retinal Pigment Epithelium Cells and Vitreous Cells. The Association for Research in Vision and Ophthalmology 2013. Seattle, USA, 2013.5.9.
2013   
Integral Analysis of Gene Signatures and MicroRNA Expression of Cultured Human Corneal Endothelial Cells in Relation to Their Functions,Cell Senescence,Epithelial-Mesenchymal Transition,and Fibrosis.The Association for Research in Vision and Ophthalmology
Asada K,Toda M,Hagiya M,Nakata K,Ueno M,Okumura N,Koizumi N,Hamuro J,Kinoshita S
2013   
Development of a Human Cultivated Nasal Mucosal Epithelial Cell Sheet for the Treatment of Severe Ocular Surface Diseases.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Kobayashi M,Nakamura T,Yasuda M,Hata Y,Okura S,Okumura N,Koizumi N,Yasu H,Kinoshita S
2013   
Activation of the Rho/ROCK Signaling Pathway in the Apoptosis of Corneal Endothelial Cells.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.
Okumura N,Odajima A,EunDuck P.Kay,Chen W,Ueno M,Hamuro J,Kinoshita S,Koizumi N
2013   
Efficacy and Safety Evaluation of Cell-Injection Therapy using Cultivated Human Corneal Endothelial Cells.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.
Koizumi N,Okumura N,Shiina T,Suzuki S,Nakamura S,Sakamoto Y,Yamasaki K,Ueno M,Hamuro J,Kinoshita S
2013   
Rho kinase inhibitor promotes cell adhesion of corneal endothelial cells through inhibiting phosphorylation of MLC.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Tsujimoto Y,Okumura N,EunDuck P.Kay,Numata R,Kinoshita S,Koizumi N
2013   
Effect of ROCK inhibiter on Apoptosis in Corneal Endothelial Cells.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Odajima A,Okumura N,EunDuck P.Kay,Chen W,Ueno M,Kinoshita S,Koizumi N
2013   
Cell-injection Therapy Using Rho Kinase Inhibitor in a Corneal Endothelial Dysfunction Rabbit Model.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Kitano J,Okumura N,EunDuck P.Kay,Ueno M,Hamuro J,Kinoshita S,Koizumi N
2013   
Proliferation Propensity of Cultured Human Corneal Endothelial Cells and Their Plasticity Dictated by Culture Microenvironments.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Toda M,Nakata K,Asada K,Hagiya M,Ueno M,Okumura N,Koizumi N,Hamuro J,Kinoshita S
2013   
Rho-kinase inhibitor enhances corneal endothelial cell proliferation via p27 degradation.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Numata R,Okumura N,EunDuck P.Kay,Nakahara M,Nakano S,Ueno M,Kinoshita S,Koizumi N
2013   
Controlled Release of a Rho Kinase (ROCK)-Selective Inhibitor with Polylactic Acid Microspheres.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Koda S,Saito T,Kitano J,Okumura N,Kinoshita S,Tabata Y,Koizumi N
2013   
Cell Line of Fuchs' Corneal Dystrophy Produces an Abnormal Extracellular Matrix.The Association for Research in Vision and Ophthalmology 2013.Seattle,USA,2013.5.6.(ポスター)
Leona Ho,Okumura N,EunDuck P.Kay,Yamasaki K,Kawasaki S,Tourtas T,Schlotzer-Schrehardt U,Kruse FE,Kinoshita S,Koizumi N
2013   
The establishment of human corneal endothelial cell culture for clinical application.第117回日本眼科学会総会,東京2013.4.4.
Okumura N
2013   
HDAC阻害薬による網膜色素上皮細胞および硝子体細胞の線維性変化への影響.第117回日本眼科学会総会,東京2013.4.4.(ポスター
畑中宏樹,小泉範子,奥村直毅,平野浩惇,水原英理,羽室淳爾,木下茂
2013   
Rhoキナーゼ阻害剤の角膜内皮創傷治癒に対する影響.第117回日本眼科学会総会,東京,2013.4.4.
小泉範子,奥村直毅,中野新一郎,北野絢嗣,太田礼,坂本雄二,羽室淳爾,上野盛夫,木下茂
2013   
Rhoキナーゼ阻害剤の角膜内皮細胞に対するアポトーシス抑制効果の検討.第117回日本眼科学会総会,東京,2013.4.4.
奥村直毅,小田嶋愛,EunDuck Kay,陳文,上野盛夫,羽室淳爾,木下茂,小泉範子
2013   
Rhoキナーゼ阻害剤を併用した培養角膜内皮細胞移植の開発.第12回日本再生医療学会総会,横浜,2013.3.21.
北野絢嗣,奥村直毅,上野盛夫,中原マキコ,沼田諒平,坂本雄二,羽室淳爾,木下茂,小泉範子
2013   
選択的Rhoキナーゼ阻害剤徐放化ポリ乳酸粒子の作製.第12回日本再生医療学会総会,横浜,2013.3.21.
迎田生,齋藤高志,北野絢嗣,奥村直毅,小泉範子,田畑泰彦
2013   
Rhoキナーゼ阻害剤の角膜内皮細胞に対する増殖促進作用機序の検討.第12回日本再生医療学会総会,横浜,2013.3.21.
沼田諒平,奥村直毅,Kay EunDuck,中原マキコ,上野盛夫,木下茂,小泉範子
2013   
Rhoキナーゼ阻害剤の培養角膜内皮細胞に対する基質接着性促進機序の検討.第12回日本再生医療学会総会,横浜,2013.3.21.
奥村直毅,辻本勇気,Kay EunDuck,沼田諒平,太田礼,上野盛夫,羽室淳爾,木下茂,小泉範子
2013   
難治性眼表面疾患に対する培養鼻粘膜上皮シートの開発.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
小林正和,中村隆宏,安田誠,畑結衣子,大倉翔貴,奥村直毅,小泉範子,久育男,木下茂
2013   
ROCK阻害剤Y-39983の角膜内皮細胞増殖に対する効果の検討.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
中野新一郎,奥村直毅,北野絢嗣,太田礼,坂本雄二,羽室淳爾,上野盛夫,木下茂,小泉範子
2013   
ポリ乳酸粒子を用いた選択的Rhoキナーゼ阻害剤徐放化の検討.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
迎田生,齋藤高志,北野絢嗣,奥村直毅,木下茂,田畑泰彦,小泉範子
2013   
Rhoキナーゼ阻害剤の培養角膜内皮細胞に対する接着促進機序の検討.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
辻本勇気,奥村直毅,Eunduck P Kay,沼田諒平,上野盛夫,羽室淳爾,木下茂,小泉範子
2013   
Rhoキナーゼ阻害剤の霊長類角膜内皮細胞に対する増殖促進機序の解明.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
沼田諒平,奥村直毅,Eunduck P Kay,中原マキ子,上野盛夫,木下茂,小泉範子
2013   
霊長類水疱性角膜症モデルを用いた培養角膜内皮細胞注入療法の有用性および安全性評価.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
奥村直毅,椎名隆, 鈴木進悟,中村紳一朗,坂本雄二,上野盛夫,羽室淳爾,木下茂,松山晃文
2013   
ウサギ水疱性角膜症モデルを用いた培養角膜内皮細胞注入療法の開発.角膜カンファランス2013第37回日本角膜学会総会・第29回日本角膜移植学会,和歌山,2013.2.14.
北野絢嗣,奥村直毅,山崎健太,中原マキ子,上野盛夫,羽室淳爾,木下茂,小泉範子
2013   
Cultivation of human corneal endothelial cells on a pericellular matrix prepared from human decidua-derived mesenchymal cells.The Association for Research in Vision and Ophthalmology 2012.Florida,USA,2012.5.10.
Numata R,Okumura N,Nakahara M,Ueno M,Kinoshita S,Kanemura Y,Sasai Y,Koizumi N
2012   
Rock inhibitor eye drops accelerate corneal endothelium wound healing in a primate model.The Association for Research in Vision and Ophthalmology 2012.Florida,USA,2012.5.10.
Okumura N,Koizumi N,Ueno M,Sakamoto Y,Takahashi H,Yamasaki K,Torii R,Hamuro J,Kinoshita S
2012   
Effect of the PPARγ agonist on the fibrotic change in primate retinal pigment epithelial cells.The Association for Research in Vision and Ophthalmology 2012.Florida,USA,2012.5.9.
Hatanaka H,Okumura N,Koizumi N,Mizuhara E,Hamuro J,Kinoshita S
2012   
Cell-injection therapy using cultivated human corneal endothelial cells in a primate model.The Association for Research in Vision and Ophthalmology 2012.Florida,USA,2012.5.7.
Koizumi N,Okumura N,Sakamoto Y,Yamasaki K,Nakahara M,Torii R,Ueno M,Hamuro J,Kinoshita S
2012   
New Therapeutic Modality of Using ROCK Inhibitor for Corneal Endothelial Disease,27th Asia-Pacific Academy of Ophthalmology Congress.2012.4.
Okumura N
2012   
Elevated keratan sulphate in the corneal stroma cultured at low oxygen tension,角膜カンファランス2012第36回日本角膜学会総会・第28回日本角膜移植学会,東京,2012.2.23.
Leona T.Y.Ho,Bruce Caterson,Keith M.Meek,Andrew J.Quantock,Okumura N,Koizumi N
2012   
The New Therapeutic Modality of Using a Rho Kinase (ROCK) Inhibitor for the Treatment of Corneal Endothelial Dysfunction.TERMIS Singapore.2011.8.5.
Okumura N
Aug 2011   
New strategy for corneal endothelial tissue engineering, Schepens Eye Research Institute 27th Biennial Cornea Conference.2011.
Koizumi N,Okumura N,Kinoshita S
2011   
ROCK inhibitor as a new therapeutic modality for corneal endothelial dysfunction.5th International Symposium of the UCSD-Doshisha Medical Imaging Research Center,San Diego,USA,2011.12.5.
Okumura N
2011   
Effect of PPARγ agonist on the fibrotic change in retinal pigment epithelium and vitreous cells.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2011.5.3.(ポスター)
Hatanaka H,Okumura N,Koizumi N,Hamuro J,Kinoshita S
2011   
ROCK Inhibitor Regulates the G1-S Progression of Corneal Endothelial Cells.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2011.5.5.
Okumura N,Koizumi N,Yamasaki K,Ueno M,Sakamoto Y,Sowa Y,Sakai T,Hamuro J,Kinoshita S
2011   
Cell-Injection Therapy using Cultivated Corneal Endothelial Cells Combined with a ROCK Inhibitor in a Primate Model.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2011.5.5.
Koizumi N,Okumura N,Yamasaki K,Ueno M,Sakamoto Y,Takahashi H,Torii R,Hamuro J,Kinoshita S
2011   
ROCK Inhibition Regulates the Cell Adhesion of Corneal Endothelial Cells.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2011.5.5.(ポスター)
Kumagai K,Koizumi N,Okumura N,Yamazaki K,Ueno M,Sakamoto Y,Yoshimura N,Hamuro J,Kinoshita S
2011   
A New Pharmaceutical Treatment for Corneal Endothelial DysfunctionAsia-ARVO 2011. Singapore. 2011.1.20.
Okumura N
2011   
The New Therapeutic Concept of Using a Rho Kinase (ROCK) Inhibitor for the Treatment of Corneal Endothelial DysfunctionKyoto Cornea Club 2010, Kyoto, Japan.2010.12.3.
Okumura N
2010   
A ROCK Inhibitor Eye Drop Enhances Corneal Endothelial Cell Proliferation.The 2nd Asia Cornea Society Biennial Scientific Meeting. Kyoto, Japan.2010.12.2.
Okumura N
2010   
The Efficacy of A ROCK Inhibitor Containing Corneal Storage Medium for Corneal Endothelial Cells.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2010.5.4.
Takahashi H,Koizumi N,Okumura N,Ueno M,Sakamoto Y,Hamuro J,Kinoshita S
2010   
The Existence of Dead Cells in Corneal Endothelium Preserved with Storage Media.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2010.5.4.
Tanioka H,Kawasaki S,Adachi H,Inatomi T,Hieda O,Fukuoka H,Okumura N,Koizumi N,Iliakis B,Kinoshita S
2010   
Cell-Injection Therapy Using Cultivated Corneal Endothelial Cells Combined With a ROCK Inhibitor in a Rabbit Model.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2010.5.5.
Koizumi N,Okumura N,Takahashi H,Ueno M,Sakamoto Y,Hirata K,Hamuro J,Kinoshita S
2010   
Feasibility Evaluation of Non-Descemet Stripping Automated Endothelial Keratoplasty by Using an In Vivo Animal Model.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2010.5.4.
Hatanaka H,Koizumi N,Okumura N,Takahashi H,Tanioka H,Kinoshita S.
2010   
The Effect of a ROCK Inhibitor on Corneal Endothelial Cell Migration in an In Vitro Wound-Healing Model.Annual Meeting of the Association for Research in Vision and Ophthalmology,Fort Lauderdale,Florida,USA.2010.5.4.
Okumura N,Koizumi N,Ueno M,Sakamoto Y,Takahashi H,Hirata K,Hamuro J,Kinoshita S
2010   
Therapeutic Potential of a Rho Kinase (ROCK) Inhibitor for the Treatment of Corneal Endothelial Dysfunction.Gordon Reserch Conference Biology & Pathobiology Of The Cornea,Ventura,USA,2010.3.7.
Okumura N,Koizumi N,Ueno M,Sakamoto Y,Takahashi H,Hirata K,Hamuro J,Kinoshita S
2010   
A ROCK inhibitor Enhances Corneal Endothelial Wound Healing in an Animal Model, The 9th Corneal Conference, Cardiff, UK, 2009. 7.15.
Okumura N,Koizumi N,Ueno M,Sakamoto Y,Takahashi H,Hirata K,Hamuro J, Kinoshita S
2009   
The Effect of Insulin and Ascorbic Acid on Rabbit Keratocyte Culture, The 9th Corneal Conference, Cardiff, UK, 2009. 7.14.
Yamamoto M,Okumura N,Hirata K,Takahashi H,Yuri Tsukahara,Toshie Isobe,and Koizumi N
2009   
A ROCK Inhibitor Enhances Corneal Endothelial Wound Healing in Both an In vitro and in vivo Model, ARVO, Florida, USA, 2009.5.6.
Okumura N,Koizumi N,Ueno M,Sakamoto Y,Takahashi H,Hirata K,Hamuro J and Kinoshita S
2009   
Cultivated monkey corneal endothelial cell transplantation using a corneal lamellar graft. ARVO, Florida, USA, 2009.5.6.
Koizumi N,Okumura N,Sakamoto Y,Takahashi H,Hirata K,Okahara N,et al
2009   
Effect of a ROCK inhibitor on corneal endothelial wound healing in an animal model. 2nd KPUM & Friedrich Alexander nurmberg Erlangen Univ Collaborating Conference. Erlangen, Germany, 2009.
Okumura N,Koizumi N,Ueno M,Sakamoto Y,Takahashi H,Hirata K,Hamuro J,Kinoshita S
2009   
Increase in Fluoroquinolone Resistance Following Long-Term Topical Use,American Academy of Opthalmology,Atlanta,USA,2008.10.
Okumura N,Sotozono C,Hashida M,Kojima K,Urabe K,Kinoshita S
2008   
A ROCK Inhibitor Enhances Primate Corneal Endothelial Cells Culture In Vitro,ARVO,Florida,USA,2008.5.
Okumura N,Ueno M,Koizumi N,Hitora H,Kinoshita S
2008   
Corneal endothelial cell proliferation is enhanced by a single injection of connexin43 siRNA in a primate endothelial injury.ARVO.2008.
Koizumi N,Nakano Y,Sakamoto Y,Okumura N,Dai P,Tsuchiya H
2008   
Expression of Cell Cycle Molecules in Primate Corneal Endothelial Cells and Primate Cultivated Corneal Endothelial Cells,ARVO,Florida,USA,2007.5.
Okumura N,Koizumi N,Matsuda A,Sakamoto Y,Funayama R,Ishikawa F,Kinoshita S
2007   
A ROCK inhibitor enhances survival of primate corneal endothelial cells in vitro,2nd KPUM & Friedrich Alexander nurmberg Erlangen Univ Collaborating Conference.Kyoto,Japan,2007.
Okumura N,Koizumi N,Ueno M,Kinoshita S
2007