Shingo Fujisaki

Profile Information

Affiliation: Professor, Faculty of Science Department of Biomolecular Science, Toho University

Degree: Doctor of Science

J-GLOBAL ID: 200901025668876804
researchmap Member ID: 1000082904

Research Interests

Research Areas

Research History

Apr, 2014 - Present

Professor, Faculty of Science Department of Biomolecular Science, Toho University
Apr, 2014 - Present

Professor, Faculty of Science, Toho University
Apr, 2000 - Apr, 2014

Associate Professor, Faculty of Science, Toho University
Apr, 2007 - Mar, 2014

Associate Professor, Faculty of Science Department of Biomolecular Science, Toho University
Oct, 1991 - Mar, 2000

Assistant Professor, Faculty of Science, Toho University
Apr, 1986 - Sep, 1991

Research Assistant, Faculty of General Education, Gifu University

Education

Apr, 1981 - Mar, 1986

Kyoto University
Apr, 1981 - Mar, 1986

Graduate School, Division of Natural Science, Kyoto University
Apr, 1977 - Mar, 1981

Faculty of Science, Kyoto University
Apr, 1977 - Mar, 1981

Faculty of Science, Kyoto University

Committee Memberships

Oct, 2014 - Present

評議員, 日本生物高分子学会

Papers

Population analysis of daptomycin-nonsusceptible methicillin-resistant Staphylococcus aureus reveals the presence of variants that contribute to daptomycin resistance.

Izumo Kanesaka†, Junya Yabuta, Tomotaka Jitsukawa, Shingo Fujisaki†, Masahiro Morita, Takashi Mikawa, Akiko Kanayama Katsuse†, Hiroshi Takahashi, Akira Ohno, Intetsu Kobayashi†

Current Microbiology, 81(61), Jan 11, 2024 Peer-reviewed
Quantification of polyprenyl diphosphates in Escherichia coli cells using high-performance liquid chromatography.

Jitsukawa, T., Watanabe, S.†, Shigeri, Y., and Fujisaki, S*†.

Bioscience, Biotechnology & Biochemistry, 88, Jan 8, 2024 Peer-reviewedCorresponding author
Liquid chromatography-mass spectrometry analyses of polyprenyl phosphates in Escherichia coli cells in which genes for isoprenoid synthesis are amplified

Murata Y, Jitsukawa T, Iida M, Furuta T†, Haramoto Y, Shigeri Y, Fujisaki S*†

Journal of bioscience and bioengineering, 135(5) 382-388, May, 2023 Peer-reviewedLast authorCorresponding author
Does sub-culturing of positive MRSA blood cultures affect vancomycin MICs?

Izumo Kanesaka†, Takamitsu Ito, Ritsuko Shishido, Makoto Nagashima†, Akiko Kanayama Katsuse†, Hiroshi Takahashi, Shingo Fujisaki†, Intetsu Kobayashi†

Journal of Medical Microbiology, 69(8) 1095-1099, Jul 8, 2020 Peer-reviewed
Construction of Staphylococcus aureus mutant deficient in the undecaprenol kinase gene, dgkA, and elucidation of the role of undecaprenol kinase in the metabolism of undecaprenyl phosphate.

Kawakami, N, Shimada, K, Fujita, T, Kanesaka, I, Kobayashi, I, Fujisaki, S

J. Biol. Macromol., 18(3) 51-58, Dec, 2018 Peer-reviewed
Characterization of compensatory mutations associated with restoration of daptomycin- susceptibility in daptomycin non-susceptible methicillin-resistant Staphylococcus aureus and the role mprF mutations

Izumo Kanesaka†, Shingo Fujisaki†, Yoshifumi Aiba, Shinya Watanabe, Takashi Mikawa, Akiko Kanayama Katsuse†, Hiroshi Takahashi†, Longzhu Cui, Intetsu Kobayashi†

Journal of Infection and Chemotherapy, 25(1) 1-5, Nov, 2018 Peer-reviewed
Undecaprenyl phosphate metabolism in Gram-negative and Gram-positive bacteria.

Kawakami, N, Fujisaki, S

Biosci. Biotech. Biochem., 82(6) 940-946, Jun, 2018 Peer-reviewed
Suppression of phenotype of Escherichia coli mutant defective in farnesyl diphosphate synthase by overexpression of gene for octaprenyl diphosphate synthase.

Takahashi, H, Aihara, Y, Ogawa, Y, Murata, Y, Nakajima, K, Iida, M, Shirai, M, Fujisaki, S

Biosci. Biotech. Biochem., 82(6) 1003-1010, Jun, 2018 Peer-reviewed
Molecular characterization of fluoroquinolone-resistant Moraxella catarrhalis variants generated in vitro by stepwise selection.

Kageto Yamada*†, Ryoichi Saito, Saori Muto, Machiko Kashiwa, Yoshiko Tamamori, Shingo Fujisaki†.

Antimicrobial agents and chemotherapy, 61(10) e01336-17, Jul 31, 2017 Peer-reviewedLast author
Non-invasive measurement of melanin-derived radicals in living mouse tail using X-band EPR

Yukihiro Ogawa, Megumi Ueno, Emiko Sekine-Suzuki, Ikuo Nakanishi, Ken-ichiro Matsumoto, Shingo Fujisaki

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 59(3) 160-164, Nov, 2016 Peer-reviewed
Daptomycin投与後に分離された同薬低感受性Methicillin-Resisftant Staphylococcus aureusの検討

金坂伊須萌, 金山明子, 宍戸律子, 内野卯津樹, 長島誠, 藤崎真吾, 小林寅喆

感染症学雑誌, 90 493-498, 2016 Peer-reviewed
Accumulation of alkaline earth metals by the green macroalga Bryopsis maxima

Shigekazu Takahashi, Kyoko Aizawa, Saki Nakamura, Katsumi Nakayama, Shingo Fujisaki, Soichiro Watanabe, Hiroyuki Satoh

BIOMETALS, 28(2) 391-400, Apr, 2015 Peer-reviewed
Identification of tomoregulin-1 as a novel addicsin-associated factor

Taku Arano, Shingo Fujisaki, Mitsushi J. Ikemoto

NEUROCHEMISTRY INTERNATIONAL, 71 22-35, May, 2014 Peer-reviewed
Mutant deletions in Escherichia coli affect the cellular levels of undecaprenyl phosphate and undecaprenyl diphosphate.

Saito, Y, Ishikawa, T, Murakami, M, Suzuki, K, Fujisaki, S

J. Biol. Macromol., 13(3) 86-91, Dec, 2013 Peer-reviewed
Involvement of an Essential Gene, mviN, in Murein Synthesis in Escherichia coli

Azusa Inoue, Yoshimitsu Murata, Hiroshi Takahashi, Naoko Tsuji, Shingo Fujisaki, Jun-ichi Kato

JOURNAL OF BACTERIOLOGY, 190(21) 7298-7301, Nov, 2008 Peer-reviewed
Short-chain prenyl diphosphate synthase that condenses isopentenyl diphosphate with dimethylallyl diphosphate in ispA Null Escherichia coli strain lacking, farnesyl diphosphate synthase

Ken Saito, Shingo Fujisaki, Tokuzo Nishino

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 103(6) 575-577, Jun, 2007 Peer-reviewed
Analysis of six new genes encoding lysis proteins and coat proteins in Escherichia coli group A RNA phages

T Nishihara, S Fujisaki, Y Nishimura, Y Minami, T Yubisui

MICROBIOLOGY AND IMMUNOLOGY, 50(1) 61-66, 2006 Peer-reviewed
Disruption of the structural gene for farnesyl diphosphate synthase in Escherichia coli

S Fujisaki, Takahashi, I, H Hara, K Horiuchi, T Nishino, Y Nishimura

JOURNAL OF BIOCHEMISTRY, 137(3) 395-400, Mar, 2005 Peer-reviewed
Interaction of the Escherichia coli lipoprotein NlpI with periplasmic Prc (Tsp) protease

A Tadokoro, H Hayashi, T Kishimoto, Y Makino, S Fujisaki, Y Nishimura

JOURNAL OF BIOCHEMISTRY, 135(2) 185-191, Feb, 2004 Peer-reviewed
Molecular cloning and heterologous expression of the C-13 phenylpropanoid side chain-CoA acyltransferase that functions in Taxol biosynthesis

K Walker, S Fujisaki, R Long, R Croteau

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 99(20) 12715-12720, Oct, 2002 Peer-reviewed

Misc.

Moraxella catarrhalisのキノロン耐性に関わる分子機構の解明

山田景土, 武藤沙起里, 齋藤良一, 藤崎真吾

日本臨床微生物学雑誌, 28(Suppl.1) 410-410, Dec, 2017
高等学校生物におけるトレーサ実験

藤崎真吾*, 石井規雄

Isotope news, 659(3月号) 12-13-13, Mar, 2009 Lead authorCorresponding author
CONDITIONS FOR OVEREXPRESSION OF CYS3 GENE OF Saccharomyces cerevisiae IN Escherichia coli AND HEAT STABILITY OF THE GENE PRODUCT

YAMAGATA Shuzo, DOI Kazuyuki, FUJISAKI Shingo, ISAJI Motomi

Bulletin of the Faculty of General Education, Gifu University, 29 147-153, 1993

Presentations

大腸菌におけるウンデカプレニルリン酸生成への大腸菌及び黄色ブドウ球菌のリピドホスファターゼ遺伝子の寄与の解析

◎末永修一, 實川智貴, 田熊ゆい, 大村梨沙子, 宮奈々恵, 飯坂洋平†, 安齊洋次郎†, 藤崎真吾†

第46回日本分子生物学会年会, Dec 8, 2023, 日本分子生物学会
イオンペア高速液体クロマトグラフィーを用いた大腸菌のポリプレニルリン酸の定量

◎實川智貴, 渡邊総一郎†, 藤崎真吾†

第46回日本分子生物学会年会, Dec 6, 2023, 日本分子生物学会
放射線がん治療の向上を目指した放射線感受性因子の日内変動解析

◎足助一真, 佐野太陽, 馬立秋, 鈴木沙彩, 小林亜利紗, 藤崎真吾†, 下川卓志

Free Radical School 2023, Nov 12, 2023, 日本酸化ストレス学会若手の会
炭素イオン線の治療効果に対する腫瘍内の日周変動の影響

◎足助一真, 佐野太陽, 馬立秋, 鈴木沙彩, 小林亜理紗, 藤崎真吾†, 下川卓志

日本放射線影響学会第66回大会, Nov 7, 2023, 日本放射線影響学会
炭素イオン線の治療効果に対する腫瘍内の日周変動の影響

◎足助一真, 佐野太陽, 馬立春, 鈴木沙彩, 小林愛理紗, 藤崎真吾†, 下川卓志

日本放射線影響学会第66回大会, Nov 4, 2023, 日本放射線影響学会
細菌の長鎖ポリプレニルリン酸および二リン酸の分別定量法の開発

◎實川智貴, 渡邊総一郎†, 茂里康, 藤崎真吾†

第33回イソプレノイド研究会例会, Sep 29, 2023, イソプレノイド研究会
イソプレノイド研究会今昔

◎藤崎真吾†

第33回イソプレノイド研究会例会, Sep 29, 2023, イソプレノイド研究会 Invited
黄色ブドウ球菌のキャリアリピド合成におけるリピドホスファターゼの寄与の解析

◎深田周平, 實川智貴, 石川諒太郎, 宮井淑充, 末永修一, 寺崎元紀, 藤崎真吾†

日本生物高分子学会2023年度大会, Sep 3, 2023, 日本生物高分子学会
黄色ブドウ球菌リピドホスファターゼ遺伝子発現の大腸菌のキャリアリピド含量および薬剤感受性への影響の解析

◎田熊ゆい, 末永修一, 實川智貴, 大村梨沙子, 宮奈々恵, 飯坂洋平†, 安齊洋次郎†, 藤崎真吾†

日本生物高分子学会2023年度大会, Sep 3, 2023, 日本生物高分子学会
Modification of lipid metabolism in Escherichia coli by expression of a lipid phosphatase gene from Staphylococcus aureus

◎Nanae Miya, RIsako Ohmura, Tomoyasu Ogura, Shuichi Suenaga, Tomotaka Jitsukawa, Shingo Fujisaki†

Sep 3, 2022, Japan Science Society of Biological Macromolecule
Improvement of lipid extraction rate in chloroform-methanol extraction from Staphylococcus aureus by cell wall degrading enzyme treatment

◎Junya Yabuta, Tomotaka Jitsukawa, Naoki Kawakami, Shingo Fujisaki†

Sep 22, 2021, The Society of Isoprenoid, Japan SIJ
Identification and Functional Analysis of the Lipid Phosphatase Genes Involved in the Dephosphorylation of Polyprenyl Diphosphates and Polyprenyl Phosphates in Staphylococcus aureus

◎Tomotaka Jitsukawa, Naoki Kawakami, Kasumi Shimada, Izumo Kanesaka†, Intetsu Kobayashi†, Shingo Fujisaki†

World microbe forum 2021, Jun 20, 2021
黄色ブドウ球菌mprF産物のウンデカプレニルリン酸輸送および分解への関与

永嶋遥花,◎實川智貴,川上直輝,金坂伊須萌†,小林寅喆†,藤崎真吾†

第437回生存圏シンポジウムイソプレノイド研究会, Sep 25, 2020, イソプレノイド研究会
黄色ブドウ球菌リピドホスファターゼ遺伝子破壊株およびウンデカプレノールキナーゼ遺伝子破壊株の脂質代謝の解析

◎實川智貴, 川上直輝, 嶋田圭純, 藤田高彬, 藤崎真吾†

第29回イソプレノイド研究会例会, Oct, 2019, イソプレノイド研究会
黄色ブドウ球菌リピドホスファターゼ遺伝子pptA, pptB破壊株の脂質代謝の解析

◎實川智貴, 川上直輝, 嶋田圭純, 藤崎真吾†

日本生物高分子学会2019年度大会, Sep, 2019, 日本生物高分子学会
炭素イオン線照射による免疫チェックポイント関連分子発現誘導の解析

◎黒澤ふき, 謝琳, 破入正行, 張明栄, 藤崎真吾†, 下川卓志

第56回アイソトープ・放射線研究発表会, Jul, 2019, 日本アイソトープ協会
Dephosphorulation of undecaprenyl diphosphate and undecaprenyl phosphate catalyzed by the pptB product of Staphylococcus aureus.

◎Naoki Kawakami†, Kasumi Shimada, Takaaki Fujita, Shingo Fujisaki†

The 2019 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, Mar 24, 2019, Japan Society for Bioscience, Biotechnology, and Agrochemistry
黄色ブドウ球菌リピドホスファターゼ欠損変異株の薬剤感受性および脂質代謝の解析

◎藤崎真吾†, 實川智貴, 川上直輝, 嶋田圭純, 藤田高彬, 金坂伊須萌†, 小林寅喆†

第15回東邦大学5学部合同学術集会, Mar 9, 2019, 5学部合同学術集会委員会
Metabolism of lipids in the Staphylococcus aureus deletion mutants of the genes for the lipid phosphatases

Nov 28, 2018
細菌の細胞壁合成リピドサイクルにおけるウンデカプレニルリン酸の供給経路ーグラム陰性菌とグラム陽性菌の比較

◎藤崎真吾†, 川上直輝†, 嶋田圭純, 藤田高彬, 實川智貴, 谷恵栄子, 齋藤雄一郎, 金坂伊須萌†, 小林寅喆†

第17回微生物研究会, Nov 17, 2018, 応用微生物学・分子細胞生物学研究奨励会

Professional Memberships

Research Projects

Elucidation of the sugar carrier lipid supply pathway to the bacterial cell wall synthesis system and its regulation

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2022 - Mar, 2025
Toward the production of a recombinant phytoremediator plant that highly accumulates environmental strontium.

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2017

SATOH Hiroyuki
Development of teaching materials and methods using uncontained radioactive isotopes for education in junior high school and/or high school

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, 2010 - 2012

SATOH Hiroyuki, FUJISAKI Shingo
原核生物における高分子のイソプレノイドによる修飾

科学研究費助成事業, 奨励研究(A), 日本学術振興会, 1992 - 1992

藤崎真吾

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