Shingo Ueno

Profile Information

Affiliation: Senior Researcher, Innovation Center of NanoMedicine (iCONM), Institute of Industry Promotion-Kawasaki
: Visiting Researcher, The Graduate School of Engineering, The University of Tokyo

Degree: Ph.D.(Saitama University)

J-GLOBAL ID: 200901060580217320
researchmap Member ID: 6000013879

External link: http://bionano.t.u-tokyo.ac.jp/

Research Interests

Research Areas

Research History

Apr, 2016 - Present

Visiting Researcher, Department of Materials Engineering, The University of Tokyo
Apr, 2015 - Present

Part-time Lecturer, Faculty of Engineering, Saitama University
Apr, 2015 - Present

Senior Researcher, Innovation Center of NanoMedicine (iCONM), Institute of Industry Promotion-Kawasaki
May, 2015 - Mar, 2016

Visiting Researcher, Department of Bioengineering, The University of Tokyo
Apr, 2012 - Mar, 2015

Project Researcher, Department of Bioengineering, The University of Tokyo
Apr, 2010 - Mar, 2012

Visiting Researcher, Department of Bioengineering, The University of Tokyo
Apr, 2010 - Mar, 2012

Project Researcher, Department of Functional Materials Science, Saitama University
Aug, 2007 - Mar, 2010

Project Researcher, Department of Regulatory Biology, Saitama University

Education

Apr, 2004 - Jul, 2007

Graduate School of Science and Engineering, Saitama University
Apr, 2002 - Mar, 2004

Graduate School of Science and Engineering, Saitama University
Apr, 1998 - Mar, 2002

Faculty of Engineering, Saitama University

Awards

Apr, 2014

優秀ポスター賞,, 酵素の人工進化を目的とした酵素固定化マイクロアレイ, 第14回東京大学生命科学シンポジウム,

上野真吾, 平井辰典, ビヤニマニッシュ, 飯塚怜, 船津高志, 一木隆範
Jun, 2013

The Photopolymer Science and Technology Award, The Best Paper Award 2013,, Photoassisted Recovery of DNA Molecules for On-chip Directed Evolution,, The Society of Photopolymer Science and Technology,

S Ueno, A Ono, R Kobayashi, Y Tanaka, S Sato, M Biyani, N Nemoto, T Ichiki
Jun, 2013

The Photopolymer Science and Technology Award, The Best Paper Award 2013, Photoassisted Recovery of DNA Molecules for On-chip Directed Evolution, The Society of Photopolymer Science and Technology

S Ueno, A Ono, R Kobayashi, Y Tanaka, S Sato, M Biyani, N Nemoto, T Ichiki
Jun, 2013

ポスター賞,, タンパク質のハイスループット解析を目的としたcDNAディスプレイマイクロアレイ, 第13回東京大学生命科学シンポジウム,

上野真吾, 小林遼, 佐藤秀介, 小野愛子, ビヤニマニッシュ, 一木隆範

Papers

Fluorometric measurement of adenosine 5′-triphosphate using exonuclease V activity

Shingo Ueno, Mika Shioya, Takanori Ichiki

Journal of Photopolymer Science and Technology, 31 in press, 2018 Peer-reviewed
Phototriggered control of enzyme reactions toward high-throughput screening on a microwell array chip

Shingo Ueno, Takanori Ichiki

Journal of Photopolymer Science and Technology, 30(6) 657-660, 2017 Peer-reviewed
Temperature-controlled microintaglio printing for high-resolution micropatterning of RNA molecules

Ryo Kobayashi, Manish Biyani, Shingo Ueno, Subhashini Raj Kumal, Hiromi Kuramochi, Takanori Ichiki

BIOSENSORS & BIOELECTRONICS, 67 115-120, May, 2015 Peer-reviewed
In situ synthesis and immobilization of enzyme molecules on microreactor array chips

Shingo Ueno, Tatsunori Hirai, Shusuke Sato, Manish Biyani, Hiromi Kuramochi, Ryo Iizuka, Takanori Akagi, Takashi Funatsu, Takanori Ichiki

Journal of Photopolymer Science and Technology, 28(5) 719-726, 2015 Peer-reviewed
Evaluation of poly(dimethylsiloxane) microreactors for pattern size miniaturization of microintaglio-printing-based protein microarray

Manish Biyani, Yoko Tanaka, Shusuke Sato, Shingo Ueno, Takanori Ichiki

JAPANESE JOURNAL OF APPLIED PHYSICS, 53(6) 06JL04, Jun, 2014 Peer-reviewed
Photo-assisted Fabrication of Ribosome Display Microarray

Subhashini Raj Kumal, Ryo Kobayashi, Shingo Ueno, Takanori Ichiki

JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, 27(4) 459-465, 2014 Peer-reviewed
Microintaglio printing of in situ synthesized proteins enables rapid printing of high-density protein microarrays directly from DNA microarrays

Manish Biyani, Junpei Moriyasu, Yoko Tanaka, Shusuke Sato, Shingo Ueno, Takanori Ichiki

Applied Physics Express, 6(8) 087001, Aug, 2013 Peer-reviewed
Simultaneous Synthesis and Biotinylation of Proteins Using Puromycin-Based Labeling Technology for Fabrication of Protein Array Chip

Subhashini Raj Kumal, Manish Biyani, Shingo Ueno, Takanori Akagi, Takanori Ichiki

JAPANESE JOURNAL OF APPLIED PHYSICS, 52(6) 06GK09, Jun, 2013 Peer-reviewed
Improvement of a puromycin-linker to extend the selection target varieties in cDNA display method

Shingo Ueno, Shinnosuke Kimura, Takanori Ichiki, Naoto Nemoto

JOURNAL OF BIOTECHNOLOGY, 162(2-3) 299-302, Dec, 2012 Peer-reviewed
In vitro selection of a peptide antagonist of growth hormone secretagogue receptor using cDNA display

Shingo Ueno, Sayaka Yoshida, Anupom Mondal, Kazuya Nishina, Makoto Koyama, Ichiro Sakata, Kenju Miura, Yujiro Hayashi, Naoto Nemoto, Koichi Nishigaki …

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(28) 11121-11126, Jul, 2012 Peer-reviewed
Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on interleukin-6-dependent cell proliferation

Naoto Nemoto, Chihiro Tsutsui, Junichi Yamaguchi, Shingo Ueno, Masayuki Machida, Toshikatsu Kobayashi, Takafumi Sakai

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 421(1) 129-133, Apr, 2012 Peer-reviewed
CDNA display: Rapid stabilization of mrna display

Shingo Ueno, Naoto Nemoto

Methods in Molecular Biology, 805 113-135, 2012 Peer-reviewed
Photoassisted Recovery of DNA Molecules for On-chip Directed Evolution

Shingo Ueno, Aiko Ono, Ryo Kobayashi, Yoko Tanaka, Shusuke Sato, Manish Biyani, Naoto Nemoto, Takanori Ichiki

JOURNAL OF PHOTOPOLYMER SCIENCE AND TECHNOLOGY, 25(1) 67-72, 2012 Peer-reviewed
An mRNA-protein fusion at N-terminus for evolutionary protein engineering

Shingo Ueno, Hidenao Arai, Miho Suzuk, Yuzuru Husimi

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 3(6) 365-374, 2007 Peer-reviewed
Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries

Tabuchi, I, S Soramoto, S Ueno, Y Husimi

BMC BIOTECHNOLOGY, 4 19, Sep, 2004 Peer-reviewed

Books and Other Publications

バイオチップの基礎と応用―原理から最新の研究・開発動向まで―

伊藤嘉浩 (Role: Contributor, 無細胞タンパク質合成系を利用したタンパク質マイクロアレイ)

株式会社シーエムシー出版, Oct, 2015
進化分子工学―高速分子進化によるタンパク質・核酸の開発―

伏見譲監修 (Role: Contributor, 進化分子工学による細胞膜受容体結合ペプチドの創製)

株式会社エヌ・ティー・エス, Oct, 2013 (ISBN: 9784864690799)

Industrial Property Rights

特開2018-117536 酵素反応の測定方法、スクリーニング方法及び測定装置

一木隆範, 上野真吾
特許第6057185号核酸リンカー

根本直人, 上野真吾, 塩野博文
特許第6048975号タンパク質固定化固相、及びポリヌクレオチド固定化固相、並びに、核酸回収方法

上野真吾, 根本直人, 一木隆範, 塩野博文, 大澤日佐雄
特許第6020865号核酸リンカー

一木隆範, 上野真吾, ビヤニマニッシュ, 小林遼, 塩野博文
特許第6015894号タンパク質アレイの製造方法及びタンパク質アレイ

根本直人, 上野真吾, 一木隆範, 塩野博文
特許第5858415号 mRNA/cDNA−タンパク質連結体作製用リンカーとそれを用いたヌクレオチド−タンパク質連結体の精製方法

根本直人, 上野真吾
特開2015-073523 核酸の検出方法、検出プローブ、マイクロアレイ、核酸検出キット、核酸‐検出プローブ‐捕捉プローブ複合体、核酸固定化担体、及び流体デバイス

一木隆範, 上野真吾, 上野太郎, 船津高志, 鈴木久皇
WO2015-029714 タンパク質アレイ又はペプチドアレイの製造方法、機能性タンパク質又は機能性ペプチドの同定方法、タンパク質アレイ又はペプチドアレイ、及び、機能性タンパク質又は機能性ペプチド同定キット

一木隆範, 上野真吾, 大澤日佐雄
特許第5697127号新規な成長ホルモン分泌促進因子受容体阻害ペプチド

坂井貴文, 上野真吾, 西垣功一, 三浦健寿
WO2014-142020 核酸リンカー

一木隆範, 上野真吾, ビヤニマニッシュ, 小林遼, 塩野博文
特開2013-192493 RNAマイクロアレイの製造方法及びRNAマイクロアレイ

塩野博文, 根本直人, 上野真吾, 一木隆範
特開2013-193988 タンパク質アレイの製造方法及びタンパク質アレイ

根本直人, 上野真吾, 一木隆範, 塩野博文
WO2013-065827 核酸リンカー

根本直人, 上野真吾, 塩野博文
WO2013-065782 タンパク質固定化固相、及びポリヌクレオチド固定化固相、並びに、核酸回収方法

上野真吾, 根本直人, 一木隆範, 塩野博文, 大澤日佐雄
特開2013-039060 酵素様活性を有するタンパク進化用リンカー及びそのリンカーを用いた酵素様活性を有するタンパクのスクリーニング方法

根本直人, 上野真吾, 福島貴之, 熊地重文
特開2012-139197 mRNA/cDNA−タンパク質連結体作製用リンカーとそれを用いたヌクレオチド−タンパク質連結体の精製方法

根本直人, 上野真吾
特開2011-182726 新規な成長ホルモン分泌促進因子受容体阻害ペプチド

坂井貴文, 上野真吾, 西垣功一, 三浦健寿

Misc.

Present Status and Trends in Development of High Density Microarray

Shingo Ueno, Manish Biyani, Shusuke Sato, Subhashini Raj Kumal, Hiromi Kuramochi, Takanori Akagi, Takanori Ichiki

BUNSEKI KAGAKU, 64(6) 421-429, Jun, 2015 Peer-reviewed
グレリン受容体（GHS-R）とアンタゴニスト

Shingo UENO, Ichiro SAKATA, Takafumi SAKAI

Seitai no kagaku, 64(5) 408-409, Oct, 2013 Invited
Molecular Design of Functional Peptides and Proteins using cDNA Display: from mRNA Display (In vitro virus) toward cDNA Display

Naoto NEMOTO, Yuki MOCHIZUKI, Shingo UENO

Seibutsu Butsuri, 53(5) 250-253, Oct, 2013 Peer-reviewed
食欲を抑制する人工ペプチドの概要

Shingo UENO

Nihon Iji Shinpo, (4616), Dec, 2012 Invited
進化分子工学的手法による成長ホルモン分泌促進因子受容体拮抗ペプチドの探索

Shingo UENO, Ichiro SAKATA, Takafumi SAKAI

Comparative Endocrinology, 38(147) 215-217, Dec, 2012 Invited
新規の摂食抑制ペプチドの創製－分子標的薬の新たな探索法を提案－

Shingo UENO, Takafumi SAKAI

埼玉大学産学官連携協議会 News Letter, 37(12) 12, Oct, 2012 Invited
医薬シーズとしての特異的結合性ペプチド(ペプチドアプタマー)の開発

西垣功一, 坂井貴文, 根本直人, 足立明人, 内田秀和, 長谷川有貴, 相田拓洋, 上野真吾, 二上雅恵, 北村幸一郎 …

埼玉大学地域オープンイノベーションセンター紀要, (2) 52-53, Jul, 2010
cDNA display法によって得られたIL‐6R結合ペプチドアプタマーのアンタゴニスト活性

根本直人, 筒井千尋, 上野真吾, 町田雅之, 坂井貴文

生化学, ROMBUNNO.3P-1172, 2010
ホルモンの病態異常と臨床検査総論 3.ホルモンの検査 6)アプタマー解析

Shingo UENO, Takafumi SAKAI

Rinsho Kensa（Journal of Medical Technology）, 52(11) 1151-1158, Oct, 2008 Invited
高速分子進化技術eRAPANSY:成長ホルモン分泌促進因子受容体に対するペプチドリガンドの試験管内選択

上野真吾, 根本直人, 根本直人, 足立明人, 西垣功一, 坂井貴文

生化学, 4P-0188, 2008
高速分子進化技術eRAPANSY:未来型創薬ツール

西垣功一, 北村幸一郎, 木下保則, 吉田昼也, SALIMULLAH Md, 辻幸香, 上野真吾, MADHU Biyani, 二上雅恵, 高橋陽子 …

生化学, 4P-0180, 2008
アプタマー解析 (ホルモンの病態異常と臨床検査) -- (ホルモンの検査)

上野真吾, 坂井貴文

臨床検査, 52(11) 1151-1158, 2008
3P275 Development of a new species of in vitro vtrus(Proteins- protein engineering, and evolutionary engineering,Poster Presentations)

Arai Hidenao, Ueno Shingo, Suzuki Miho, Husimi Yuzuru

Biophysics, 47(1) S271, Nov 20, 2007
3P076 New species of in vitro virus

Arai H, Ueno S, Suzuki M, Husimi Y

Biophysics, 45(1) S222, Oct 19, 2005
3P101 Linkage between the N-terminus of a peptide and its encoding mRNA

Ueno S, Hukaisi K, Arai H, Suzuki M, Husimi Y

Biophysics, 44(1) S215, Nov 10, 2004
High quality Random DNA Libraries for evolutionary protein engineering

Ueno S, Tabuchi I, Soramoto S, Hukaisi K, Arai H, Husimi Y

Biophysics, 43(1) S79, Aug 25, 2003
1M1645 A random DNA library with any controlled codon usage

Soramoto S, Tabuchi I, Ueno S, Husimi Y

Biophysics, 42(2) S79, Oct 10, 2002

Profile Information

Research Interests

Research Areas

Research History

Education

Awards

Papers

Books and Other Publications

Industrial Property Rights

Misc.

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