Kawai Gota - My portal

Profile Information

Affiliation: Professor, Chiba Institute of Technology

Degree: 理学博士(東京大学)

Researcher number: 70211860
J-GLOBAL ID: 200901032995296222
researchmap Member ID: 5000100162

External link: https://cit-lifescience.jp/kawai/index.html

Research Interests

Research Areas

Research History

Apr, 2020 - Present

Professor, Chiba Institute of Technology
Apr, 2016 - Present

Professor, Faculty of Advanced Engineering, Department of Life Science, Chiba Institute of Technology
Apr, 2010 - Present

Professor, Chiba Institute of Technology
Apr, 2010 - Present

Chiba Institute of Technology
Apr, 2010 - Present

Chiba Institute of Technology
Apr, 2010 - Mar, 2020

Professor, Chiba Institute of Technology
Apr, 1997 - Mar, 2016

Professor, Faculty of Engineering, Department of Life and Environmental Science, Chiba Institute of Technology

Committee Memberships

Jan, 2010 - Present

Councilor, The Japanese Society of Polyamine Research
Apr, 2020 - Mar, 2023

副会長, 日本核磁気共鳴学会
Apr, 2017 - Mar, 2023

理事, 日本核磁気共鳴学会
Apr, 2009 - Mar, 2015

理事, 日本核磁気共鳴学会
Jan, 2010

Councilor, The Japanese Society of Polyamine Research
Apr, 2002 - Mar, 2006

会計幹事, 日本RNA学会
Jun, 1995 - May, 2003

編集委員, 日本分光学会

Awards

2014

ＪＢ論文賞, 日本生化学会
Oct, 2007

ＪＢ論文賞, 日本生化学会

Papers

Strict Interactions of Fifth Letters, Hydrophobic Unnatural Bases, in XenoAptamers with Target Proteins

Michiko Kimoto, Hui Pen Tan, Ken-ichiro Matsunaga, Nur Afiqah Binte Mohd Mislan, Gota Kawai, and Ichiro Hirao

J. Am. Chem. Soc, 145(37) 20432-20441, 2023 Peer-reviewed
Non-coding RNAs and functional RNA elements in Thermus thermophilus,

Kawai, G., Sampei, G., Nishiyama, M. and Bessho, Y.

J. Gen. Appl. Microbiol., 69, 2023 Peer-reviewedLead authorCorresponding author
Interaction between a fluoroquinolone derivative KG022 and RNAs: Effect of base pairs 3ʹ adjacent to the bulged residues

Rika Ichijo, Takashi Kamimura and Gota Kawai

Front. Mol. Biosci., 10 1145528, 2023 Peer-reviewedLast authorCorresponding author
Cis-Allosteric Regulation of HIV-1 Reverse Transcriptase by Integrase

Takao Masuda, Osamu Kotani, Masaru Yokoyama, Yuya Abe, Gota Kawai and Hironori Sato

Viruses, 15 31, 2023 Peer-reviewed
T-hairpin structure found in the RNA element involved in piRNA biogenesis

Naomi Takase, Maina Otsu, Shigeki Hirakata, Hirotsugu Ishizu, Mikiko C. Siomi, and Gota Kawai

RNA, 28 541-550, 2022 Peer-reviewed
Interaction between a fluoroquinolone derivative and RNAs with a single bulge

Konami Nagano, Takashi Kamimura and Gota Kawai

J. Biochem., 171 239-244, 2022 Peer-reviewed
Dye-Conjugated Spinach RNA by Genetic Alphabet Expansion

Kyung Hyun Lee, Michiko Kimoto, Gota Kawai, Itaru Okamoto, Andrea Fin, and Ichiro Hirao

Chem. Eur. J., 28 e202104396, 2022 Peer-reviewed
2-Amino-1,8-naphthyridine Dimer (ANP77), a High-Affinity Binder to the Internal Loops of C/CC and T/CC Sites in Double-Stranded DNA

Bimolendu Das, Konami Nagano, Gota Kawai, Asako Murata, and Kazuhiko Nakatani

The Journal of Organic Chemistry, 87 340-350, 2022 Peer-reviewed
Identification of a short form of a Caenorhabditis elegans Y RNA homolog Cel7 RNA

Takehiro Chiba; Shin-ya Kihara; Manami Sato; Kou Xingkui; Simon Goto; Takuma Suzumura; Hyouta Himeno; Gota Kawai

BBRC, 557 104-109, 2021
Developing an Updated Strategy for Estimating the Free-Energy Parameters in RNA Duplexes

Wayne K. Dawson, Amiu Shino, Gota Kawai and Ella Czarina Morishita

Int. J. Mol. Sci., 22 9708, 2021 Peer-reviewed
Small molecule targeting r(UGGAA)n disrupts RNA foci and alleviates disease phenotype in Drosophila model

Tomonori Shibata, Konami Nagano, Morio Ueyama, Kensuke Ninomiya, Tetsuro Hirose, Yoshitaka Nagai, Kinya Ishikawa, Gota Kawai and Kazuhiko Nakatani

Nature Communications, 12(1) 236, 2021
Influence of the 5’‑terminal sequences on the 5’‑UTR structure of HIV‑1 genomic RNA

Camille Michiko Obayashi, Yoko Shinohara, Takao Masuda and Gota Kawai

Scientific Reports, 11 10920, 2021 Peer-reviewedCorresponding author
Epigenetic TET Catalyzed Oxidative Products of 5-Methylcytosine Impede Z-DNA Formation of CG Decamers

Vongsutilers, Vorasit; Shinohara, Yoko; Kawai, Gota

ACS Omega, 5 8056-8064, 2020 Peer-reviewed
Cytotoxic Mechanism of Excess Polyamines Functions through Translational Repression of Specific Proteins Encoded by Polyamine Modulon

Akihiko Sakamoto, Junpei Sahara, Gota Kawai, Kaneyoshi Yamamoto, Akira Ishihama, Takeshi Uemura, Kazuei Igarashi, Keiko Kashiwagi and Yusuke Terui

Int. J. Mol. Sci., 21(7) 2406, 2020 Peer-reviewed
Impact of 5'-end nucleotide modifications of HIV-1 genomic RNA on reverse transcription

Huang, Y.-L., Kawai, G., Hasegawa, A., Kannagi, M. and Masuda, T.

Biophysical Research Communications, 516(4) 1145-1151, Sep 3, 2019 Peer-reviewed
Crystal structure of the flavin-dependent thymidylate synthase Thy1 from Thermus thermophilus with an extra C-terminal domain

Ogawa, A., Sampei, G. and Kawai, G.

Acta Crystallographica Section F, 75 450-454, Jun, 2019 Peer-reviewed
Inhibition of pre-miRNA-136 processing by Dicer with small molecule BzDANP suggested the formation of ternary complex of pre-miR-136–BzDANP–Dicer

Otabe, T., Nagano, K., Kawai, G., Murata, A., Nakatani

Bioorganic & Medicinal Chemistry, 27 2140-2148, May 15, 2019 Peer-reviewed
Distinct RNA recognition mechanisms in closely related LINEs from zebrafish

Otsu, M. and Kawai, G.

Nucleosides, Nucleotides, and Nucleic Acids, 38(4) 294-304, Apr 3, 2019 Peer-reviewed
Synthesis of a protected ribonucleoside phosphoramidite-linked spin label via an alkynyl chain at the 5' position of uridine

Hatano, A., Terado, N., Kanno, Y., Nakamura, T. and Kawai, G.

Synthetic Communications, 49(1) 136-145, 2019 Peer-reviewed
Conformational Changes in HIV-1 Reverse Transcriptase that Facilitate Its Maturation

Ryan L. Slack, Tatiana V. Ilina, Zhaoyong Xi, Nicholas S. Giacobbi, Gota Kawai, Michael A. Parniak, Stefan G. Sarafianos, Nicolas Sluis Cremer, and Rieko Ishima

Structure, 27 1581-1593, 2019 Peer-reviewed

Books and Other Publications

Essential Structural Biology

Gota Kawai, Taiichi Sakamoto, Naoki Nemoto (Role: Joint author, Chapters 1, 4.3, 5)

Kodansha, Feb, 2018
Experimental Approaches of NMR spectroscopy

Taiichi Sakamoto, Maina Otsu and Gota Kawai (Role: Joint author, Chapter 16 NMR Studies on RNA pp439-459)

Springer, Jan, 2018
NMR分光法

(Role: Joint author, 第3章 3.1, 3.2, 3.3)

講談社, Apr, 2016
機能性RNAの分子生物学

河合剛太,清澤秀孔編 (Role: Joint author)

クバプロ, Dec, 2010
核磁気共鳴分光法

河合剛太ほか (Role: Joint author)

講談社サイエンティフィク, Jul, 2009
機能性Non-coding RNA

河合剛太,金井昭夫編 (Role: Joint author)

クバプロ, Apr, 2006
タンパク質工学の基礎

河合剛太ほか (Role: Joint author)

東京化学同人, Sep, 2004
NMR分光法原理から応用まで

河合剛太ほか (Role: Joint author)

学会出版センター, Jan, 2003

Presentations

近縁のLINEにおける異なるRNA認識⽅法のNMR法による解析

⼤津舞菜, 河合剛太

第92回日本生化学会大会, Sep 20, 2019, 日本生化学会
HIV-1ゲノムRNAの5’末端の違いが構造と機能に与える影響

⼤林カミーユ美智⼦, 篠原陽⼦, 増⽥貴夫, 河合剛太

第92回日本生化学会大会, Sep 20, 2019, 日本生化学会
piRNAの生成に関与するRNA elementの二次構造解析

髙瀬直美, 大津舞菜,石津大嗣, 塩見春彦, 塩見美喜子, 河合剛太

日本RNA学会年会, Jul 17, 2019, 日本ＲＮＡ学会
NGS解析による高度好熱菌からの新規small non-coding RNAの探索

青木優里, 小池奈緒美,齊藤有紀, 三瓶嚴一,河合剛太

第41回日本分子生物学会年会, Nov 30, 2018, 日本分子生物学会
マウス由来新規低分子RNAの探索およびその構造解析

鈴村拓馬,清澤秀孔,牛田千里,河合剛太

第41回日本分子生物学会年会, Nov 29, 2018, 日本分子生物学会
RNAの構造モチーフと低分子化合物認識能

野村伊織,篠阿弥宇,渡辺彩雪香,長野来南,上村孝,中村慎吾,河合剛太

第41回日本分子生物学会年会, Nov 28, 2018, 日本分子生物学会
Thermus thermophilus 由来のチミジル酸合成酵素の立体構造とC末端ドメインの機能の解析

小川青葉,三瓶嚴一,河合剛太

第41回日本分子生物学会年会, Nov 28, 2018, 日本分子生物学会
RNA 部分構造の抽出およびNMR 法による低分子化合物との相互作用解析

河合剛太

第399 回CBI 学会講演会「RNA を標的とした低分子創薬研究」, Nov 14, 2018, 情報計算化学生物学会（CBI 学会） Invited
Analysis of interaction between quinolone derivatives and bulge regions of RNA

Ayuka Watanabe, Konami Nagano, Takashi Kamimura, Shingo Nakamura, Gota Kawai

ISNAC2018, Nov 8, 2018, Japan Society of Nucleic Acids Chemistry
近縁のLINEにおける異なるRNA認識方法

大津舞菜,河合剛太

第91回日本生化学会大会, Sep 26, 2018, 日本生化学会
HIV-1ゲノムRNAにおける5’末端のG残基の数によるTAR-PolyA領域の立体構造の違い

篠原陽子,増田貴夫,河合剛太

第91回日本生化学会大会, Sep 25, 2018, 日本生化学会
UGGAAリピート配列を含むRNAと低分子化合物naphthyridine carbamate dimerとの相互作用の解析

長野来南,柴田知範,中谷和彦,河合剛太

第91回日本生化学会大会, Sep 24, 2018, 日本生化学会
互いに識別し合う2種のLINEにおける相互作用の解析

大津舞菜,河合剛太

第57回NMR討論会（2018）, Sep 18, 2018, 日本核磁気共鳴学会
フルオロキノロン誘導体とRNAとの相互作用解析

長野来南,上村孝,中村慎吾,河合剛太

第57回NMR討論会（2018）, Sep 18, 2018, 日本核磁気共鳴学会
フルオロキノロン誘導体とRNAとの相互作用解析

渡辺彩雪香,長野来南,篠原陽子,上村孝,中村慎吾,河合剛太

第20回日本RNA学会年会, Jul 10, 2018, 日本RNA学会
ゼブラフィッシュ由来のLINEのRNAの構造と逆転写酵素による認識方法

大津舞菜,河合剛太

2018年度日本生化学会関東支部例会, Jun 23, 2018, 日本生化学会関東支部
Interaction analysis of Fluoroquinolone derivatives and RNA by NMR

Konami Nagano, Shinya Tokuoka, Takashi Kamimura, Shingo Nakamura, Gota Kawai

Consortium of Biological Sciences 2017, Dec 6, 2017, Consortium of Biological Sciences
RNomics of Thermus themophilus HB8 by DNA microarray and next-generation sequencing

Gota Kawai, Yuri Aoki, Maina Otsu, Naomi Koike, Gen-ichi Sampei

Consortium of Biological Schences 2017, Dec 6, 2017, Consortium of Biological Schences
Secondary structural analysis of small molecule non-coding RNA expressed from a region preserved in introns in mammals

Takuma Suzumura, Hidenori Kiyosawa, Gota kawai

The 56th Annual Meeting of the Nuclear Magnetic Resonance Society of Japan, Nov 14, 2017, The Nuclear Magnetic Resonance Society of Japan
Solution structure of a reverse transcriptase recognition site of a LINE RNA from zebrafish

Maina Otsu, Masaki Kajikawa, Norihiro Okada and Gota Kawai

19th RNA meeting in Toyama, Jul 21, 2017, The RNA Society of Japan

Professional Memberships

Aug, 2019 - Present

The Chem-Bio Informatics Society
Jan, 2010 - Present

The Japanese Society of Polyamine Research
- Mar, 2020

日本分光学会
日本分子生物学会
日本生化学会
日本核磁気共鳴学会
日本RNA学会

Research Projects

NMR法による相互作用解析および結合サイトの同定

国立研究開発法人日本医療研究開発機構, Dec, 2021
HIV多機能分子が制御する新規感染機構の根幹構造の解明

国立研究開発法人日本医療研究開発機構, Apr, 2021
長鎖RNAの機能構造を発見するための技術基盤の開発とその応用

私立大学戦略的研究基盤形成支援事業（研究拠点を形成する研究）, 文部科学省, Jun, 2011 - Mar, 2016
Secondary structure-based analysis of small RNA from mouse brain

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

KAWAI Gota, KIYOSAWA Hidenori, USHIDA Chisato
Multi-disciplinary analysis ob 50-100nt-sized,small RNAs Universally found in mouse cells

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project), Japan Society for the Promotion of Science, Apr, 2009 - Mar, 2012

KIYOSAWA Hidenori, KAWAI Gota, USHIDA Chisato
機能性長鎖ncRNAのNMR構造解析技術の顕在化

産学共同シーズイノベーション化事業顕在化ステージ, 科学技術振興機構, Aug, 2008 - Jul, 2009
RNAタンパク質複合体構造（「RNA情報網」計画研究）

特定領域研究, 文部科学省, Apr, 2002 - Mar, 2007
Functional analysis of proteins from thermophiles

Jul, 2002 - Mar, 2006
Discovery and application of functional non-coding RNAs

Jun, 2002 - Mar, 2005
Structural biology of RNA

Jun, 1997 - Mar, 2002

Industrial Property Rights

7,038,036 Oligonucleotides labeled with Stable Isotopes and Method for detecting the same
US2006051804 Oligonucleotides labeled with Stable Isotopes and Method for detecting the same
特開2000-290291 安定同位体標識オリゴヌクレオチド及びオリゴヌクレオチド検出方法
特開平07-115987 安定同位体標識RNAの製造方法

河合剛太, 渡部暁, 岡田務, 飯野寿美子, 福田裕章
特開平10-165193 Ｄ標識５'リボヌクレオチド及びその製造方法

福田裕章, 岡田務, 河合剛太
特開2006-20575 RNA安定化剤ならびにこのRNA安定化剤を用いたRNA安定化方法および複合体安定化方法