論文

査読有り
2010年3月

Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Maya Shirakura
  • ,
  • Keiji Tanimoto
  • ,
  • Hidetaka Eguchi
  • ,
  • Mutsumi Miyauchi
  • ,
  • Hideaki Nakamura
  • ,
  • Keiko Hiyama
  • ,
  • Kotaro Tanimoto
  • ,
  • Eiji Tanaka
  • ,
  • Takashi Takata
  • ,
  • Kazuo Tanne

393
4
開始ページ
800
終了ページ
805
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2010.02.086
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Vascular endothelial growth factor (Vegf) was previously shown to be expressed specifically in the condylar cartilage of temporomandibular joint-osteoarthritis (TMJ-OA) model rats. Here we demonstrate for the first time that hypoxia-inducible factor-1 alpha (Hif-1 alpha) is activated in mature chondrocytes of temporomandibular joint-osteoarthritis (TMJ-OA) model rat by mechanical overload, and that activated Hif-1 in chondrocytes can induce osteoclastogenesis via repression of osteoprotegerin (Opg) expression.
In rat TMJs, degeneration of the condylar cartilage became prominent in proportion to the duration of overloading. Hif-1 alpha expression was observed specifically in mature and hypertrophic chondrocytes, and Hif-1 alpha-positivity, level of Vegf expression, and tartrate-resistant acid phosphatase (TRAP)-positive cell numbers all increased in the same manner. When ATDC5 cells induced differentiation by insulin were cultured under hypoxia, Hif-1 alpha induction was observed in mature stage, but not in immature stage. Inductions of Hif-1-target genes showed a similar expression pattern. In addition, expression of Opg decreased in hypoxia, and Hif-1 alpha played a role, in part, in its regulation. (C) 2010 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 27

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2010.02.086
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000276053800044&DestApp=WOS_CPL