論文

査読有り
2017年4月

Transplanting mouse induced pluripotent stem cells into mouse otocysts in vivo

NEUROSCIENCE LETTERS
  • Hiroki Takeda
  • ,
  • Ryosei Minoda
  • ,
  • Toru Miwa
  • ,
  • Takao Yamada
  • ,
  • Momoko Ise

647
開始ページ
153
終了ページ
158
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.neulet.2017.03.014
出版者・発行元
ELSEVIER IRELAND LTD

The otocyst is an attractive target for studying treatment strategies for genetic hearing loss and for understanding inner ear development. We have previously reported that trans-uterine supplemental gene therapy in vivo into the otocysts of mice, which had a loss of function mutation in a causative gene of deafness, was able to prevent putative hearing loss. We herein set out to clarify the feasibility of allogenic cell transplantation into the mouse otocysts in vivo. We transplanted naive mouse-derived induced pluripotent stem cells (miPSCs) into the otocysts of wild type mice or connexin (Cx) 30 deficient mice, at embryonic day 11.5 (E11.5). The transplanted m-iPSCs survived in the lumens of the inner ears at E13.5 and E15.5 in wild type mice. In the Cx30 deficient mouse, the transplanted cells survived similarly, with some of the transplanted cells migrating into the lining cells of the lumens of the inner ears at E13.5 and showing tumorigenic cell proliferation at E15.5. In addition, engrafted cells appear to be able to differentiate after the cell transplantation.
Our results suggest that otocyst transplanted cells survived and differentiated. A Cx30 deficiency may facilitate cell migration. These findings may offer some hope for cell transplantation therapy for profound genetic hearing loss caused by a Cxs deficiency. (C) 2017 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.neulet.2017.03.014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28359931
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401679800025&DestApp=WOS_CPL

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