論文

査読有り
2011年12月

Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides

NEUROREPORT
  • Toru Miwa
  • ,
  • Ryosei Minoda
  • ,
  • Taku Kaitsuka
  • ,
  • Momoko Ise
  • ,
  • Kazuhito Tomizawa
  • ,
  • Eiji Yumoto

22
18
開始ページ
994
終了ページ
999
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1097/WNR.0b013e32834da8f8
出版者・発行元
LIPPINCOTT WILLIAMS & WILKINS

The mouse otocyst, an anlage of the inner ear, is an attractive experimental target for developing treatment modalities for congenital inner ear diseases and for studying inner ear development. Poly-arginine (6-12 residues) is a cell-penetrating peptide and can be used to deliver cargo into cells. Here, we achieved transutero delivery of enhanced green fluorescent protein (EGFP) fused to a nine-arginine peptide into mouse embryonic otocysts. The EGFP signal was detected both in the lining cells of the otocysts and in their vicinity at 18 h post injection. Mice injected with EGFP fused to a nine-arginine peptide had normal auditory and vestibular functions. These data suggest that protein transduction using poly-arginine may be a useful alternative strategy to commonly used gene delivery methods for delivering therapeutically relevant molecules to the developing inner ear. NeuroReport 22:994-999 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1097/WNR.0b013e32834da8f8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22045255
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000297221200012&DestApp=WOS_CPL

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