論文

査読有り
2017年4月

Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

SCIENTIFIC REPORTS
  • Shigeo S. Sugano
  • ,
  • Hiroko Suzuki
  • ,
  • Eisuke Shimokita
  • ,
  • Hirofumi Chiba
  • ,
  • Sumihare Noji
  • ,
  • Yuriko Osakabe
  • ,
  • Keishi Osakabe

7
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-017-00883-5
出版者・発行元
NATURE PUBLISHING GROUP

Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryopreserved protoplasts, we identified a novel promoter, CcDED1(pro), with seven times stronger activity in this assay than the conventional promoter GPD2. To develop highly efficient genome editing using CRISPR/Cas9 in C. cinerea, we used the CcDED1(pro) to express Cas9 and a U6-snRNA promoter from C. cinerea to express gRNA. Finally, CRISPR/Cas9-mediated GFP mutagenesis was performed in a stable GFP expression line. Individual genome-edited lines were isolated, and loss of GFP function was detected in hyphae and fruiting body primordia. This novel method of high-throughput CRISPR/Cas9-based genome editing using cryopreserved protoplasts should be a powerful tool in the study of edible mushrooms.

Web of Science ® 被引用回数 : 48

リンク情報
DOI
https://doi.org/10.1038/s41598-017-00883-5
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28455526
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000400247600019&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/s41598-017-00883-5
  • ISSN : 2045-2322
  • PubMed ID : 28455526
  • Web of Science ID : WOS:000400247600019

エクスポート
BibTeX RIS