論文

査読有り
2011年4月

Secondary neurulation: Fate-mapping and gene manipulation of the neural tube in tail bud

Development Growth & Differentiation
  • Eisuke Shimokita
  • ,
  • Yoshiko Takahashi

Vol.53
No.3
開始ページ
401
終了ページ
410
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1440-169X.2011.01260.x

The body tail is a characteristic trait of vertebrates, which endows the animals with a variety of locomotive functions. During embryogenesis, the tail develops from the tail bud, where neural and mesodermal tissues make a major contribution. The neural tube in the tail bud develops by the process known as secondary neurulation (SN), where mesenchymal cells undergo epithelialization and tubulogenesis. These processes contrast with the well known primary neurulation, which is achieved by invagination of an epithelial cell sheet. In this study we have identified the origin of SN-undergoing cells, which is located caudo-medially to Hensen's node of early chicken embryo. This region is distinctly fate-mapped from tail-forming mesoderm. The identification of the presumptive SN region has allowed us to target this region with exogenous genes using in ovo electroporation techniques. The SN-transgenesis has further enabled an exploration of molecular mechanisms underlying mesenchymal-to-epithelial transition during SN, where activity levels of Cdc42 and Rac1 are critical. This is the first demonstration of molecular and cellular analyses of SN, which can be performed at a high resolution separately from tail-forming mesoderm.

リンク情報
DOI
https://doi.org/10.1111/j.1440-169X.2011.01260.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21492152
ID情報
  • DOI : 10.1111/j.1440-169X.2011.01260.x
  • ISSN : 1440-169X
  • PubMed ID : 21492152

エクスポート
BibTeX RIS