論文

査読有り 筆頭著者 責任著者
2018年6月4日

A 5′ UTR-Overlapping LncRNA Activates the Male-Determining Gene doublesex1 in the Crustacean Daphnia magna

Current Biology
  • Yasuhiko Kato
  • ,
  • Christelle Alexa G. Perez
  • ,
  • Nur Syafiqah Mohamad Ishak
  • ,
  • Quang D. Nong
  • ,
  • Yuumi Sudo
  • ,
  • Tomoaki Matsuura
  • ,
  • Tadashi Wada
  • ,
  • Hajime Watanabe

28
11
開始ページ
1811
終了ページ
1817.e4
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.cub.2018.04.029
出版者・発行元
Cell Press

Long noncoding RNAs (lncRNAs) are pervasively transcribed in the eukaryotic genome [1] and are important for the control of master regulatory genes that are involved in cell differentiation and development [2, 3]. Here, we show that a 5′ UTR-overlapping lncRNA regulates the male-specific expression of the DM-domain gene doublesex1 (dsx1) in the crustacean Daphnia magna, which produces males in response to environmental stimuli. This lncRNA, named doublesex1 alpha promoter-associated long RNA (DAPALR), is transcribed upstream the transcription start site (TSS) in a sense orientation and subjected to 5′ end capping and 3′ end processing at a stem-loop structure before the dsx1 coding exon. Similar to dsx1, its expression is only activated in males by the juvenile hormone (JH) and basic-leucine zipper (bZIP) transcription factor Vrille (Vri) and is maintained during embryogenesis. Knockdown of DAPALR in males silenced dsx1 and led to feminization, including egg production, whereas ectopic expression of DAPALR in dsx1-silenced females resulted in the de-repression of dsx1. We further demonstrate that the DAPALR transcript overlaps the dsx1 5′-UTR, and this overlapping region is required for dsx1 activation. Our results suggest that DAPALR can transactivate and possibly maintain dsx1 expression. This might be important for converting transient environmental signals into stable male development, controlled by the continuous expression of dsx1. The DM domain gene dsx1 is exclusively expressed in males of the crustacean Daphnia magna. Kato et al. demonstrate that the lncRNA DAPALR activates dsx1 in trans. It overlaps dsx1 5′ UTR, and this overlapping region is required for dsx1 activation. This work will improve understanding of the biogenesis and function of sense-overlapping lncRNAs.

リンク情報
DOI
https://doi.org/10.1016/j.cub.2018.04.029
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29804806
ID情報
  • DOI : 10.1016/j.cub.2018.04.029
  • ISSN : 0960-9822
  • PubMed ID : 29804806
  • SCOPUS ID : 85047179771

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