KAZUKI KAWAHARA

Profile Information

Affiliation: -

Degree: 博士（薬学）(大阪大学)

J-GLOBAL ID: 201801019712168049
researchmap Member ID: B000321363

External link: http://www.phs.osaka-u.ac.jp/homepage/b001/

Research Areas

Life sciences / Structural biochemistry /

Papers

A yeast two-hybrid system to obtain triple-helical ligands from combinatorial random peptide libraries

Ryo Masuda, Khine Phyu Phyu Thant, Kazuki Kawahara, Hiroya Oki, Tetsuya Kadonosono, Yuji Kobayashi, Takaki Koide

bioRxiv, https://doi.org/10.1101/2023.11.14.567114, Nov, 2023
Contribution of a surface salt bridge to the protein stability of deep-sea Shewanella benthica cytochrome c'

Sotaro Fujii, Riku Sakaguchi, Hiroya Oki, Kazuki Kawahara, Tadayasu Ohkubo, So Fujiyoshi, Yoshihiro Sambongi

Journal of Structural Biology, 108031-108031, Sep, 2023
Structural basis for the toxin-coregulated pilus-dependent secretion of Vibrio cholerae colonization factor.

Hiroya Oki, Kazuki Kawahara, Minato Iimori, Yuka Imoto, Haruka Nishiumi, Takahiro Maruno, Susumu Uchiyama, Yuki Muroga, Akihiro Yoshida, Takuya Yoshida …

Science advances, 8(41) eabo3013, Oct 14, 2022 Peer-reviewed
Organic Materials Used for Giant Buddhas and Wall Paintings in Bamiyan, Afghanistan

Yoko Taniguchi, Kazuki Kawahara, Miho Takashima, Marine Cotte, Joy Mazurek, Yuki Kumazawa, Yuki Taga, Takashi Nakazawa

Applied Sciences, 12(19) 9476-9476, Sep 21, 2022 Peer-reviewed
Crystal structure of thermally stable homodimeric cytochrome c'-β from Thermus thermophilus.

Taisuke Yoshimi, Sotaro Fujii, Hiroya Oki, Takeshi Igawa, Hannah R Adams, Kengo Ueda, Kazuki Kawahara, Tadayasu Ohkubo, Michael A Hough, Yoshihiro Sambongi

Acta crystallographica. Section F, Structural biology communications, 78(Pt 6) 217-225, Jun 1, 2022 Peer-reviewed
Analysis of the complete genome sequences of Clostridium perfringens strains harbouring the binary enterotoxin BEC gene and comparative genomics of pCP13-like family plasmids

Kengo Ueda, Kazuki Kawahara, Narumi Kimoto, Yusuke Yamaguchi, Kazuhiro Yamada, Hiroya Oki, Takuya Yoshida, Shigeaki Matsuda, Yuki Matsumoto, Daisuke Motooka, Kentaro Kawatsu, Tetsuya Iida, Shota Nakamura, Tadayasu Ohkubo, Shinya Yonogi

BMC Genomics, 23(1) 226-226, Mar, 2022 Peer-reviewed
Substrate-induced product-release mechanism of lipocalin-type prostaglandin D synthase

Shigeru Shimamoto, Yusuke Nakagawa, Yuji Hidaka, Takahiro Maruno, Yuji Kobayashi, Kazuki Kawahara, Takuya Yoshida, Tadayasu Ohkubo, Kosuke Aritake, Mahesh K. Kaushik …

Biochemical and Biophysical Research Communications, 569 66-71, Sep, 2021 Peer-reviewed
Spatiotemporal regulation of PEDF signaling by type I collagen remodeling.

Kazuki Kawahara, Takuya Yoshida, Takahiro Maruno, Hiroya Oki, Tadayasu Ohkubo, Takaki Koide, Yuji Kobayashi

Proceedings of the National Academy of Sciences of the United States of America, 117(21) 11450-11458, May 26, 2020 Peer-reviewed
Structural Basis for PPARα Activation by 1H-pyrazolo-[3,4-b]pyridine Derivatives.

Takuya Yoshida, Hiroya Oki, Michihiro Doi, Syohei Fukuda, Tomohiro Yuzuriha, Ryotaro Tabata, Kenji Ishimoto, Kazuki Kawahara, Tadayasu Ohkubo, Hiroyuki Miyachi …

Scientific reports, 10(1) 7623-7623, May 6, 2020 Peer-reviewed
Stability of cytochromes c' from psychrophilic and piezophilic Shewanella species: implications for complex multiple adaptation to low temperature and high hydrostatic pressure.

Suka A, Oki H, Kato Y, Kawahara K, Ohkubo T, Maruno T, Kobayashi Y, Fujii S, Wakai S, Lisdiana L …

Extremophiles, 23(2) 239-248, Mar, 2019 Peer-reviewed
N-terminal HCV core protein fragment decreases 20S proteasome activity in the presence of PA28γ.

Yang Zheng, Shigeru Shimamoto, Takahiro Maruno, Yuji Kobayashi, Yoshiharu Matsuura, Kazuki Kawahara, Takuya Yoshida, Tadayasu Ohkubo

Biochemical and biophysical research communications, 509(2) 590-595, Feb, 2019 Peer-reviewed
Interplay of a secreted protein with type IVb pilus for efficient enterotoxigenic Escherichia coli colonization.

Hiroya Oki, Kazuki Kawahara, Takahiro Maruno, Tomoya Imai, Yuki Muroga, Shunsuke Fukakusa, Takaki Iwashita, Yuji Kobayashi, Shigeaki Matsuda, Toshio Kodama …

Proceedings of the National Academy of Sciences of the United States of America, 115(28) 7422-7427, Jul 10, 2018 Peer-reviewed
BEC, a novel enterotoxin produced by non-CPE producing Type A Clostridium perfringens, is a cause of human gastroenteritis

Shinya Yonogi, Shigeaki Matsuda, Takao Kawai, Tomoko Yoda, Tetsuya Harada, Yuko Kumeda, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Kazuki Kawahara, Ryota Munetomo …

Sep, 2017
Structural and functional insights into thermally stable cytochrome c' from a thermophile

Sotaro Fujii, Hiroya Oki, Kazuki Kawahara, Daisuke Yamane, Masaru Yamanaka, Takahiro Maruno, Yuji Kobayashi, Misa Masanari, Satoshi Wakai, Hirofumi Nishihara …

PROTEIN SCIENCE, 26(4) 737-748, Apr, 2017 Peer-reviewed
Type IV pilus structure and assembly of human enterotoxigenic Escherichia coli

Hiroya Oki, Kazuki Kawahara, Shunsuke Fukakusa, Takuya Yoshida, Takahiro Maruo, Yuji Kobayashi, Tetsuya Iida, Tadayasu Ohkubo, Shota Nakamura

Feb, 2017
Crystal structure of the ADP-ribosylating component of BEC, the binary enterotoxin of Clostridium perfringens

Kazuki Kawahara, Shinya Yonogi, Ryota Munetomo, Hiroya Oki, Takuya Yoshida, Yuko Kumeda, Shigeaki Matsuda, Toshio Kodama, Tadayasu Ohkubo, Tetsuya Iida …

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 480(2) 261-267, Nov, 2016 Peer-reviewed
Homo-trimeric Structure of the Type IVb Minor Pilin CofB Suggests Mechanism of CFA/III Pilus Assembly in Human Enterotoxigenic Escherichia coli

Kazuki Kawahara, Hiroya Oki, Shunsuke Fukakusa, Takuya Yoshida, Tomoya Imai, Takahiro Maruno, Yuji Kobayashi, Daisuke Motooka, Tetsuya Iida, Tadayasu Ohkubo …

JOURNAL OF MOLECULAR BIOLOGY, 428(6) 1209-1226, Mar, 2016 Peer-reviewed
Ordered self-assembly of the collagenous domain of adiponectin with noncovalent interactions via glycosylated lysine residues

Ayako Takuwa, Takuya Yoshida, Takahiro Maruno, Kazuki Kawahara, Masayoshi Mochizuki, Yuji Nishiuchi, Yuji Kobayashi, Tadayasu Ohkubo

FEBS LETTERS, 590(2) 195-201, Jan, 2016 Peer-reviewed
Comparative study on stabilization mechanism of monomeric cytochrome c(5) from deep-sea piezophilic Shewanella violacea

Misa Masanari, Sotaro Fujii, Kazuki Kawahara, Hiroya Oki, Hirofumi Tsujino, Takahiro Maruno, Yuji Kobayashi, Tadayasu Ohkubo, Satoshi Wakai, Yoshihiro Sambongi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(12) 2365-2370, 2016 Peer-reviewed
Structural Basis for Dimer Formation of Human Condensin Structural Maintenance of Chromosome Proteins and Its Implications for Single-stranded DNA Recognition

Susumu Uchiyama, Kazuki Kawahara, Yuki Hosokawa, Shunsuke Fukakusa, Hiroya Oki, Shota Nakamura, Yukiko Kojima, Masanori Noda, Rie Takino, Yuya Miyahara …

JOURNAL OF BIOLOGICAL CHEMISTRY, 290(49) 29461-U428, Dec, 2015 Peer-reviewed

Misc.

ジスルフィド結合ではない分子内架橋による酵素のレドックススイッチ

河原一樹

ファルマシア, 58(2) 171-171, Feb, 2022 Peer-reviewedLead author
等温滴定型熱測定による睡眠物質合成酵素と基質の相互作用解析

島本茂, 中川悠介, 日高雄二, 丸野孝浩, 小林祐次, 河原一樹, 吉田卓也, 大久保忠恭, 有竹浩介, 裏出良博

日本細胞生物学会大会(Web), 71st, 2019
腸管毒素原性大腸菌（ETEC）が産生するIV型線毛の立体構造解析

河原一樹, 沖大也, 深草俊輔, 吉田卓也, 今井友也, 丸野孝浩, 小林祐次, 元岡大祐, 飯田哲也, 大久保忠恭 …

日本結晶学会平成28年度年会, Nov, 2016
高分解能ESI-QTOF-MSを用いたモノクローナル抗体の酸化修飾の評価

丸野孝浩, 河原一樹, 野田勝紀, 山本岳, 瀧浪欣彦, 大久保忠恭, 内山進, 内山進

質量分析総合討論会講演要旨集, 64th, 2016
リポカリン型プロスタグランジンD合成酵素(L-PGDS)とリガンド複合体の構造解析

沖大也, 島本茂, 秦殊斌, 加藤信幸, 元岡大祐, 中村昇太, 河原一樹, 吉田卓也, 大久保忠恭

日本結晶学会年会講演要旨集, 2015, 2015
Structural Analysis of the CFA/III Minor Pilin Subunit CofB of Human Enterotoxigenic Escherichia coli

Hiroya Oki, Kazuki Kawahara, Shunsuke Fukakusa, Takuya Yoshida, Yuji Kobayashi, Tooru Taniguchi, Takeshi Honda, Tetsuya Iida, Shota Nakamura, Tadayasu Ohkubo

PROTEIN SCIENCE, 23 101-101, Jul, 2014
Structure-Activity Relationship of Marinostatin, an Ester-Linked Serine Protease Inhibitor

TAICHI Misako, YAMAZAKI Toshimasa, KAWAHARA Kazuki, NAKAMURA Shota, MOTOOKA Daisuke, TESHIMA Tadashi, OHKUBO Tadayasu, KOBAYASHI Yuji, NISHIUCHI Yuji

2010 52-52, Mar 1, 2011
The Crystal Structure of the Collagen Model Peptide containing 4(S)-hydroxyproline-proline-glycine Repeats

MOTOOKA Daisuke, KAWAHARA Kazuki, NAKAMURA Shota, DOI Masamitsu, NISHIUCHI Yuji, NAKAZAWA Takashi, OHKUBO Tadayasu, KOBAYSHI Yuji

2010 153-153, Mar 1, 2011
Thermodynamic analysis of PPARγ-ligand interaction

MARUNO Takahiro, TAKAHASHI Ryo, HOSOKAWA Chiho, MOTOOKA Daisuke, KAWAHARA Kazuki, NISHIMURA Mitsuhiro, NISHIUCHI Yuji, NAKAMURA Shota, UCHIYAMA Susumu, YOSHIDA Takuya …

2010 294-294, Mar 1, 2011
Chemical synthesis and structure-function studies of marinostatin, a potent serine protease inhibitor

M. T. Taichi, T. Yamazaki, K. Kawahara, D. Motooka, S. Nakamura, T. Teshima, T. Ohkubo, Y. Kobayashi, Y. Nishiuchi

JOURNAL OF PEPTIDE SCIENCE, 16 42-43, Sep, 2010
2P-1098 Interactive analysis of human SMC hinge domain

MIYAHARA Yuya, SAIKI Naoya, NODA Masanori, UCHIYAMA Susumu, MATSUNAGA Sachihiro, KAWAHARA Kazuki, NAKAMURA Syota, HOSOKAWA Yuki, OHKUBO Tadayasu, FUKUI Kiichi

22 33-33, 2010
4S-ヒドロキシプロリンを含むコラーゲンモデルペプチドの結晶構造解析

元岡大祐, 元岡大祐, 河原一樹, 中村昇太, 土井正光, 西内祐二, 中沢隆, 大久保忠恭, 小林祐次

日本結晶学会年会講演要旨集, 2009, 2009
Effect of Hydration on the Thermal Stability of the Collagen Model Peptide Containing 4(S)-Hydroxyproline

NISHI Yoshinori, KAWAHARA Kazuki, UCHIYAMA Susumu, DOI Masamitsu, NISHIUCHI Yuji, NAKAZAWA Takashi, OHKUBO Tadayasu, KOBAYASHI Yuji

2006 169-170, Mar 1, 2007
コラーゲンの三本鎖構造の熱安定性に及ぼす水和の効果

西義則, 西義則, 内山進, 河原一樹, 土井正光, 西内祐二, 中沢隆, 大久保忠恭, 小林祐次, 小林祐次

熱測定討論会講演要旨集, 43rd, 2007
¹⁹F-NMRを用いたコラーゲンモデルペプチドのフォールディング機構の研究

河原一樹, 根本暢明, 元岡大祐, 西義則, 土井正光, 内山進, 中沢隆, 西内祐二, 吉田卓也, 大久保忠恭 …

Abstracts. Annual Meeting of the NMR Society of Japan, 46th, 2007
F-19-NMR study of collagen model peptides containing 4(R)-fluoroproline

K. Kawahara, N. Nemoto, T. Yoshida, T. Ohkubo, Y. Nishiuchi, T. Nakazawa, M. Doi, S. Uchiyama, Y. Nishi, Y. Kobayashi

BIOPOLYMERS, 88(4) 516-516, 2007
Stabilizing Mechanism of Collagen-like Triple Helical Structure of (4(R)-Hydroxyprolyl-4(R)-hydroxyprolyl-glycine)_<10>

NISHI Yoshinori, KAWAHARA Kazuki, NAKAMURA Shota, UCHIYAMA Susumu, NISHIUCHI Yuji, NAKAZAWA Takashi, OHKUBO Tadayasu, KOBAYASHI Yuji

2005 393-396, Mar 1, 2006
¹⁹F-NMRを用いた4(R)-fluoroprolineを含むコラーゲンモデルペプチドに関する研究

河原一樹, 根本暢明, 西義則, 土井正光, 内山進, 中沢隆, 西内祐二, 吉田卓也, 大久保忠恭, 小林祐次

Abstracts. Annual Meeting of the NMR Society of Japan, 45th, 2006
Effect of hydration on the thermal stability of the collagen triple helix

K. Kawahara, Y. Nishi, S. Nakamura, S. Uchiyama, M. Doi, Y. Nishiuchi, T. Nakazawa, T. Ohkubo, Y. Kobayashi

JOURNAL OF PEPTIDE SCIENCE, 12 198-198, 2006

Research Projects

Investigating the roles of type IV pilus system in regulating enteric infection of bacterial pathogens

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2021 - Mar, 2024
Genomic survey of enteric pathogens in India for developing anti-adhesion therapies

Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Japan Society for the Promotion of Science, Oct, 2020 - Mar, 2023
Interplay of a secreted protein with type IV pilus for efficient adhesion of enteric pathogens

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Kawahara Kazuki
Oversea's Research on Archaeological Proteins as Molecular Markers of Ancient Culture and Civilization

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2020

Nakazawa Takashi
Research on the animal glue production in ancient East Asia

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2014 - Mar, 2018

MIYAJI ATSUKO
プロテオミクス技術による古代タンパク質分析

科学研究費助成事業新学術領域研究(研究領域提案型), 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2015 - Mar, 2017

河原一樹
Amino acid sequencing of animal glues remained in cultural properties by proteomic techniques

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2013 - Mar, 2016

KAWAHARA KAZUKI
Identification of Animal Species of Collagen Used as Glues in Archaeological Materials Utilizing MALDI Mass Spectrometry

Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up, Grant-in-Aid for Research Activity Start-up, Japan Society for the Promotion of Science, 2010 - 2011

KAWAHARA Kazuki
コラーゲン三重螺旋構造の熱安定性とフォールディング機構の解明

科学研究費助成事業特別研究員奨励費, 特別研究員奨励費, 日本学術振興会, 2008 - 2009

河原一樹

Profile Information

Research Areas

Papers

Misc.

Research Projects

Menu

Coauthors