1990年12月
ESTABLISHMENT OF A PHYLOGENETIC SURVEY SYSTEM FOR AIDS-RELATED LENTIVIRUSES AND DEMONSTRATION OF A NEW HIV-2 SUBGROUP
AIDS
- 巻
- 4
- 号
- 12
- 開始ページ
- 1257
- 終了ページ
- 1261
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1097/00002030-199012000-00012
- 出版者・発行元
- RAPID SCIENCE PUBLISHERS
We designed a universal primer (UNIPOL) for DNA amplification of AIDS-related viruses. The phylogenetic tree constructed from the presumed sequences amplified with UNIPOL was representative of the tree calculated from whole pol gene sequences so far reported. UNIPOL was able to amplify the sequences of all four major groups of primate lentiviruses and also that of a distinct virus from a Ghanaian patient with an AIDS-related complex, designated GH-2. This strain scarcely hybridizes with known HIV/simian immunodeficiency virus (SIV) DNA probes. Sequence analysis of the only amplified fragment revealed rapidly that GH-2 was quite similar to the recently reported HIV-2ALT(D205) and that these two viruses form a new subgroup distinct from known HIV-2 and SIV(mac)/SIV(sm) in the large HIV-2 group. This system will be useful for further phylogenetic study of various primate lentiviruses.
- リンク情報
- ID情報
-
- DOI : 10.1097/00002030-199012000-00012
- ISSN : 0269-9370
- PubMed ID : 1965125
- Web of Science ID : WOS:A1990ER95000012