論文

査読有り
2014年3月

Demonstration of marmosets (Callithrix jacchus) as a non-human primate model for secondary dengue virus infection: high levels of viraemia and serotype cross-reactive antibody responses consistent with secondary infection of humans

JOURNAL OF GENERAL VIROLOGY
  • Meng Ling Moi
  • ,
  • Tomohiko Takasaki
  • ,
  • Tsutomu Omatsu
  • ,
  • Shinichiro Nakamura
  • ,
  • Yuko Katakai
  • ,
  • Yasushi Ami
  • ,
  • Yuriko Suzaki
  • ,
  • Masayuki Saijo
  • ,
  • Hirofumi Akari
  • ,
  • Ichiro Kurane

95
PART3
開始ページ
591
終了ページ
600
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/vir.0.060384-0
出版者・発行元
SOC GENERAL MICROBIOLOGY

There are four dengue virus (DENV) serotypes. Primary infection with one does not confer protective immunity against the others. We have reported previously that the marmoset (Callithrix jacchus) is a useful primary DENV infection model. It has been reported that secondary DENV infection with a heterotypic serotype induces viraemia kinetics and antibody responses that differ from those in primary infection. Thus, it is important to determine the utility of the marmoset as a model for secondary DENV infection. Marmosets were infected with heterologous DENV by secondary inoculation, and viraemia kinetics and antibody responses were analysed. The marmosets consistently developed high levels of viraemia after the secondary inoculation with heterologous DENV serotypes. IgM responses were lower compared with primary inoculation responses, whilst IgG responses were rapid and high. Neutralizing activities, which possessed serotype cross-reactive activities, were detected as early as 4 days after inoculation. In addition, infectious viraemia titres were higher when assayed with Fc gamma receptor-expressing baby hamster kidney (BHK) cells than when assayed with conventional BHK cells, suggesting the presence of infectious virus antibody immune complexes. After secondary infection with heterotypic DENV, the marmosets demonstrated viraemia kinetics, IgM and IgG responses, and high levels of serotype cross-reactive neutralizing antibody responses, all of which were consistent with secondary DENV infection in humans. The results indicate the marmoset as a useful animal for studying secondary, as well as primary, DENV infection.

リンク情報
DOI
https://doi.org/10.1099/vir.0.060384-0
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000338177500008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1099/vir.0.060384-0
  • ISSN : 0022-1317
  • eISSN : 1465-2099
  • Web of Science ID : WOS:000338177500008

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