MISC

2014年

Specific Detection of Escherichia coli by Using Metallic Mesh Sensor in THz Region

2014 XXXITH URSI GENERAL ASSEMBLY AND SCIENTIFIC SYMPOSIUM (URSI GASS)
  • Ippei Kurita
  • ,
  • Tetsuhito Suzuki
  • ,
  • Naoshi Kondo
  • ,
  • Takashi Kondo
  • ,
  • Seiji Kamba
  • ,
  • Yoshiko Miura
  • ,
  • Yuichi Ogawa

978-1-4673-5225-3
開始ページ
14693170
終了ページ
記述言語
英語
掲載種別
DOI
10.1109/URSIGASS.2014.6930066
出版者・発行元
IEEE

We demonstrated specific and rapid detection of Escherichia coli (E. coli) without culturing and labeling, using a metallic mesh sensor. Streptavidin and biotinylated antibody, specific for E. coli, were immobilized on the metallic mesh surface via amination and maleimidation. E. coli in PBS were captured, and attached to the surface of the metallic mesh, by immunoreaction with the antibody for E. coli. Transmission spectra of these samples were then measured around 10 THz, where the size of the generated, localized, electric field is the same as that of E. coli. After demonstrating a significant relationship between the frequency shift on the metallic mesh sensor and antibody for E. coli concentration, we then went on to specifically and quantitatively measure E. coli concentration. As E. coli concentration increased so did the spectral frequency shift, with detection limit being observed at 10(6) mL(-1).

リンク情報
DOI
https://doi.org/10.1109/URSIGASS.2014.6930066
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000366628703250&DestApp=WOS_CPL
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84919741127&origin=inward
ID情報
  • DOI : 10.1109/URSIGASS.2014.6930066
  • SCOPUS ID : 84919741127
  • Web of Science ID : WOS:000366628703250

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