2014年8月
Metal Mesh Device Sensor Immobilized with a Trimethoxysilane-Containing Glycopolymer for Label-Free Detection of Proteins and Bacteria
ACS APPLIED MATERIALS & INTERFACES
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- 巻
- 6
- 号
- 15
- 開始ページ
- 13234
- 終了ページ
- 13241
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/am503003v
- 出版者・発行元
- AMER CHEMICAL SOC
Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10(-9) to 10(-6) mol L-1 using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm(-2) was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.
- リンク情報
- ID情報
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- DOI : 10.1021/am503003v
- ISSN : 1944-8244
- eISSN : 1944-8252
- PubMed ID : 25014128
- Web of Science ID : WOS:000340446300167