論文

国際誌
2021年1月26日

A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America
  • Kazuhiro Murakami
  • ,
  • Yumi Terakado
  • ,
  • Kikue Saito
  • ,
  • Yoshie Jomen
  • ,
  • Haruna Takeda
  • ,
  • Masanobu Oshima
  • ,
  • Nick Barker

118
4
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.2016806118
出版者・発行元
Proceedings of the national academy of sciences

An ability to safely harness the powerful regenerative potential of adult stem cells for clinical applications is critically dependent on a comprehensive understanding of the underlying mechanisms regulating their activity. Epithelial organoid cultures accurately recapitulate many features of in vivo stem cell-driven epithelial renewal, providing an excellent ex vivo platform for interrogation of key regulatory mechanisms. Here, we employed a genome-scale clustered, regularly interspaced, short palindromic repeats (CRISPR) knockout (KO) screening assay using mouse gastric epithelial organoids to identify modulators of Wnt-driven stem cell-dependent epithelial renewal in the gastric mucosa. In addition to known Wnt pathway regulators, such as Apc, we found that KO of Alk, Bclaf3, or Prkra supports the Wnt independent self-renewal of gastric epithelial cells ex vivo. In adult mice, expression of these factors is predominantly restricted to non-Lgr5-expressing stem cell zones above the gland base, implicating a critical role for these factors in suppressing self-renewal or promoting differentiation of gastric epithelia. Notably, we found that Alk inhibits Wnt signaling by phosphorylating the tyrosine of Gsk3β, while Bclaf3 and Prkra suppress regenerating islet-derived (Reg) genes by regulating the expression of epithelial interleukins. Therefore, Alk, Bclaf3, and Prkra may suppress stemness/proliferation and function as novel regulators of gastric epithelial differentiation.

リンク情報
DOI
https://doi.org/10.1073/pnas.2016806118
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33479180
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848749
ID情報
  • DOI : 10.1073/pnas.2016806118
  • ORCIDのPut Code : 87273271
  • PubMed ID : 33479180
  • PubMed Central 記事ID : PMC7848749

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