MISC

2014年1月1日

Amyloid beta (A4) precursor protein expression in human periodontitis-affected gingival tissues

Archives of Oral Biology
  • T. Kubota
  • ,
  • T. Kubota
  • ,
  • S. Maruyama
  • ,
  • D. Abe
  • ,
  • T. Tomita
  • ,
  • T. Morozumi
  • ,
  • N. Nakasone
  • ,
  • T. Saku
  • ,
  • T. Saku
  • ,
  • H. Yoshie

59
開始ページ
586
終了ページ
594
DOI
10.1016/j.archoralbio.2014.03.004

Objective Periodontitis involves periodontal tissue destruction and is associated with chronic inflammation and ageing. Periodontitis has recently been recognised as a risk factor for Alzheimer's disease (AD). We showed upregulation of molecules in the AD pathway including amyloid beta (A4) precursor protein (APP), a key gene in AD, interleukin-1 beta (IL-1β), and complement component 1 (q subcomponent, A chain) (C1QA) in periodontitis compared to healthy tissues. Here, we quantitatively analysed the expression levels of APP, IL-1β, and C1QA and determined the localisation of APP in gingival tissues. Design Fourteen chronic periodontitis patients and 14 healthy participants were enrolled. Six samples of total RNA from two distinct sites of healthy and periodontitis-affected gingival tissues from three randomly selected patients were used for microarray analyses, and significant biological pathways in periodontitis were identified. Differential gene expression of APP, IL-1β, and C1QA, which belong to the AD pathway, were analysed with quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR) using samples from these 14 chronic periodontitis patients and 14 healthy controls. APP localisation was analysed with immunohistochemistry. Results APP, IL-1β, and C1QA mRNA levels were significantly upregulated in periodontitis-affected gingival tissues. APP was mainly localised in macrophages in gingival connective tissues underneath the epithelial layers. Conclusions An association between AD and periodontitis was detected with microarray and computer-aided data mining analyses. qRT-PCR identified differential gene expression in periodontitis-affected gingival tissue that may be related to AD pathogenesis. Elevated APP, IL-1β, and C1QA transcripts and APP-expressing macrophages in periodontitis-affected gingival tissues were observed, suggesting a relationship between periodontitis and AD pathogenesis. © 2014 Elsevier Ltd.

リンク情報
DOI
https://doi.org/10.1016/j.archoralbio.2014.03.004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24690593
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84897430294&origin=inward
ID情報
  • DOI : 10.1016/j.archoralbio.2014.03.004
  • ISSN : 0003-9969
  • PubMed ID : 24690593
  • SCOPUS ID : 84897430294

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