論文

査読有り
2016年4月

Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma

PATHOLOGY
  • Ahmed Abdelaziz Mohamed Essa
  • ,
  • Manabu Yamazaki
  • ,
  • Satoshi Maruyama
  • ,
  • Tatsuya Abe
  • ,
  • Hamzah Babkair
  • ,
  • Adel Mohamed Raghib
  • ,
  • Eman Money El-Din Megahed
  • ,
  • Jun Cheng
  • ,
  • Takashi Sakui

48
3
開始ページ
219
終了ページ
227
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.pathol.2016.02.006
出版者・発行元
LIPPINCOTT WILLIAMS & WILKINS

To confirm our hypothesis that macrophages recruited to fight against oral squamous cell carcinoma (SCC) invasion are functionally differentiated within neoplastic stromata, we analysed arrangements of macrophage subtypes and cancer-associated fibroblasts (CAFs) in their association with blood vasculatures in the neoplastic stroma. Surgical specimens of oral SCC were immunohistochemically examined for macrophage phenotypes (CD68, CD163, and CD204) and stromal environments (perlecan, connexin 43, and CD31). Human monocytes were co-cultured with ZK-1 cells of oral SCC origin in different culture conditions. SCC stromata were divided into two types: fascicular (fibroblast rich) and reticular (perlecan-rich). Regardless of stromal types, CD68 positive (+)/CD163+/CD204+ macrophages were recruited when blood vessels were abundant. Connexin 43+ fibroblasts were enriched in the fascicular stroma, where blood vessels were depleted. In co-culture experiments, monocytes, in the presence of ZK-1 cells, showed TNF-alpha(low)/IL-12(low) and IL-10(high)/VEGF(high)/MMP-9(high) with increased expression levels for fibronectin and perlecan. With direct contact with monocytes, SCC cells also expressed CD68 and CD163. SCC stromata were characterised by CD163+/CD204+ tumour-associated macrophages (TAMs) and connexin 43+ CAFs. TAMs are differentiated from monocytes by the physical contact with oral SCC cells in the perlecan-rich neoplastic stroma, which is also induced by the cross-talk between SCC cells and stromal cells including TAMs.


リンク情報
DOI
https://doi.org/10.1016/j.pathol.2016.02.006
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27020496
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000374437700004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.pathol.2016.02.006
  • ISSN : 0031-3025
  • eISSN : 1465-3931
  • PubMed ID : 27020496
  • Web of Science ID : WOS:000374437700004

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