論文

査読有り
2016年

Differential immunohistochemical expression profiles of perlecan-binding growth factors in epithelial dysplasia, carcinoma in situ, and squamous cell carcinoma of the oral mucosa

PATHOLOGY RESEARCH AND PRACTICE
  • Mayumi Hasegawa
  • ,
  • Jun Cheng
  • ,
  • Satoshi Maruyama
  • ,
  • Manabu Yamazaki
  • ,
  • Tatsuya Abe
  • ,
  • Hamzah Babkair
  • ,
  • Chikara Saito
  • ,
  • Takashi Saku

212
5
開始ページ
426
終了ページ
436
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.prp.2016.02.016
出版者・発行元
ELSEVIER GMBH, URBAN & FISCHER VERLAG

The intercellular depdsit of perlecan, a basement-membrane type heparan sulfate proteoglycan, is considered to function as a growth factor reservoir and is enhanced in oral epithelial dysplasia and carcinoma in situ (CIS). However, it remains unknown which types of growth factors function in these perlecan-enriched epithelial conditions. The aim of this study was to determine immunohistochemically which growth factors were associated with perlecan in normal oral epithelia and in different epithelial lesions from dysplasia and CIS to squamous cell carcinoma (SCC). Eighty-one surgical tissue specimens of oral SCC containing different precancerous stages, along with ten of normal mucosa, were examined by immunohistochemistry for growth factors. In normal epithelia, perlecan and growth factors were not definitely expressed. In epithelial dysplasia, VEGF, SHH, KGF, Flt-1, and Flk-1 were localized in the lower half of rete ridges (in concordance with perlecan, 33-100%), in which Ki-67 positive cells were densely packed. In CIS, perlecan and those growth factors/receptors were more strongly expressed in the cell proliferating zone (63-100%). In SCC, perlecan and KGF disappeared from carcinoma cells but emerged in the stromal space (65-100%), while VEGF, SHH, and VEGF receptors remained positive in SCC cells (0%). Immunofluorescence showed that the four growth factors were shown to be produced by three oral SCC cell lines and that their signals were partially overlapped with perlecan signals. The results indicate that perlecan and its binding growth factors are differentially expressed and function in specific manners before (dysplasia/CIS) and after (SCC) invasion of dysplasia/carcinoma cells. (C) 2016 Elsevier GmbH. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.prp.2016.02.016
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26965914
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000375816500011&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.prp.2016.02.016
  • ISSN : 0344-0338
  • PubMed ID : 26965914
  • Web of Science ID : WOS:000375816500011

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