論文

査読有り
2015年7月

Protease-activated receptor 2 modulates proliferation and invasion of oral squamous cell carcinoma cells

HUMAN PATHOLOGY
  • Kamal Al-Eryani
  • ,
  • Jun Cheng
  • ,
  • Tatsuya Abe
  • ,
  • Satoshi Maruyama
  • ,
  • Manabu Yamazaki
  • ,
  • Hamzah Babkair
  • ,
  • Ahmed Essa
  • ,
  • Takashi Saku

46
7
開始ページ
991
終了ページ
999
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.humpath.2015.03.003
出版者・発行元
W B SAUNDERS CO-ELSEVIER INC

Based on our previous finding that protease-activated receptor 2 (PAR-2) regulates hemophagocytosis of oral squamous cell carcinoma (SCC) cells, which induces their heme oxygenase 1- dependent keratinization, we have formulated a hypothesis that PAR-2 functions in wider activities of SCC cells. To confirm this hypothesis, we investigated immunohistochemical profiles of PAR-2 in oral SCC tissues and its functional roles in cell proliferation and invasion in SCC cells in culture. The PAR-2 expression modes were determined in 48 surgical tissue specimens of oral SCC. Using oral SCC derived cell systems, we determined both gene and protein expression levels of PAR-2. SCC cell proliferation and invasive properties were also examined in conditions in which PAR-2 was activated by the synthetic peptide SLIGRL. PAR-2 was immunolocalized in oral SCC and carcinoma in situ cells, especially in those on the periphery of carcinoma cell foci (100% of cases), but not in normal oral epithelia. Its expression at both gene and protein levels was confirmed in 3 oral SCC cell lines including ZK-1. Activation of PAR-2 induced ZK-1 cell proliferation in a dose-dependent manner. PAR-2 activated ZK-1 cells invaded faster than nonactivated ones. The expression of PAR-2 is specific to oral malignancies, and PAR-2 regulates the growth and invasion of oral SCC cells. (C) 2015 Elsevier Inc. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.humpath.2015.03.003
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25912757
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356910300008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.humpath.2015.03.003
  • ISSN : 0046-8177
  • eISSN : 1532-8392
  • PubMed ID : 25912757
  • Web of Science ID : WOS:000356910300008

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