論文

査読有り
2015年11月

Simultaneous immunolocalization of desmoglein 3 and IgG4 in oral pemphigus vulgaris: IgG4 predominant autoantibodies in its pathogenesis

JOURNAL OF ORAL PATHOLOGY & MEDICINE
  • Tatsuya Abe
  • ,
  • Satoshi Maruyama
  • ,
  • Hamzah Babkair
  • ,
  • Manabu Yamazaki
  • ,
  • Jun Cheng
  • ,
  • Takashi Saku

44
10
開始ページ
850
終了ページ
856
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/jop.12290
出版者・発行元
WILEY-BLACKWELL

BackgroundOral pemphigus vulgaris (PV), an autoimmune blistering disease, is mainly mediated by autoantibodies against desmoglein (Dsg) 3. However, no attention has been paid to IgG subclasses of the autoantibodies against Dsg3 in the diagnostic procedure for PV. Thus, our aim in this study was to investigate whether Dsg3 and any of IgG subclasses are immunohistochemically colocalized in tissue sections of PV oral mucosa.
Materials and MethodsSerial sections cut from formalin-fixed paraffin blocks of biopsy specimens of 9 PV cases and those of normal buccal mucosa surgically removed for fibro-epithelial polyps were comparatively examined for immunohistochemical localizations for Dsg3, IgG4, and IgG.
ResultsDsg3 was demonstrated in a dot-like pattern on the cell border and in the cytoplasm of the whole epithelial layer in both normal and PV specimens, while its staining was irregular among floating epithelial sheets of PV. IgG4 was also demonstrated in a punctuated fashion on the cell border among floating epithelial sheets, which was nearly identical to the immunohistochemical profile of Dsg3. In addition to being detected in the epithelial part, IgG4 signals were prominently localized in plasma cells scattered in the granulation tissue, where ratios of IgG4-positive (+) plasma cells to IgG+ cells were extraordinarily higher (mean 28%) than those in normal mucosa.
DiscussionThese findings confirmed for the first time that autoantibodies against Dsg3 are mainly composed of IgG4 in oral PV and that the combined immunohistochemistry for Dsg3 and IgG4 can be a valuable aid in confirming a histopathological diagnosis of PV.


リンク情報
DOI
https://doi.org/10.1111/jop.12290
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25401705
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000363866100012&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/jop.12290
  • ISSN : 0904-2512
  • eISSN : 1600-0714
  • PubMed ID : 25401705
  • Web of Science ID : WOS:000363866100012

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