論文

査読有り
2014年11月

Keratin pearl degradation in oral squamous cell carcinoma: reciprocal roles of neutrophils and macrophages

JOURNAL OF ORAL PATHOLOGY & MEDICINE
  • Ahmed A. M. Essa
  • ,
  • Manabu Yamazaki
  • ,
  • Satoshi Maruyama
  • ,
  • Tatsuya Abe
  • ,
  • Hamzah Babkair
  • ,
  • Jun Cheng
  • ,
  • Takashi Saku

43
10
開始ページ
778
終了ページ
784
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/jop.12197
出版者・発行元
WILEY-BLACKWELL

BackgroundWe have reported that neutrophilic infiltration was associated with round-shaped dyskeratosis foci, a kind of keratin pearl, of oral carcinoma in situ and that those inflammatory cells are recruited from intra-epithelially entrapped blood vessels. Based on these lines of evidence, we have formulated a hypothesis that keratin pearls are terminally degraded by neutrophils. To confirm this hypothesis, we investigated immunohistochemically stepwise degradation of keratin pearls in oral squamous cell carcinoma (SCC) to clarify any other type scavenger cells in addition to neutrophils are involved in this particular degradation process.
MethodsNeutrophils (neutrophil elastase) and macrophage subpopulations (CD68, CD163 and CD204) were immunohistochemically localized in 30 cases of oral SCC with typical round-shaped keratin pearls. SCC cells were revealed by immunohistochemistry for keratin (K) 17, and blood vessels were demonstrated by CD31.
ResultsKeratin pearl degradation process was divided into four steps: (i) intact stage: no macrophage infiltration but minimal neutrophils were found in keratin pearls; (ii) neutrophil recruit stage: no macrophage infiltration but focal neutrophilic infiltration within the pearls; (iii) neutrophil predominant stage: dense neutrophil infiltration with minimal macrophages and segregated keratinized cancer cells strongly positive for K17; and (iv) macrophage predominant stage: dense infiltration of CD68-, CD163 (mononuclear)- and CD204 (multinucleated)-positive macrophages engulfing detached keratinized SCC cells.
ConclusionKeratin pearl degradation in oral SCC is strictly regulated by two types of scavenger cells: neutrophils, which perform initial tasks, and macrophages, which reciprocally take over from neutrophils the role to finalize the degradation processes.


リンク情報
DOI
https://doi.org/10.1111/jop.12197
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24931829
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000344543000009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1111/jop.12197
  • ISSN : 0904-2512
  • eISSN : 1600-0714
  • PubMed ID : 24931829
  • Web of Science ID : WOS:000344543000009

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