論文

査読有り
2013年8月

Podoplanin-mediated cell adhesion through extracellular matrix in oral squamous cell carcinoma

LABORATORY INVESTIGATION
  • Masayuki Tsuneki
  • ,
  • Manabu Yamazaki
  • ,
  • Satoshi Maruyama
  • ,
  • Jun Cheng
  • ,
  • Takashi Saku

93
8
開始ページ
921
終了ページ
932
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/labinvest.2013.86
出版者・発行元
NATURE PUBLISHING GROUP

Podoplanin (PDPN), one of the representative mucin-like type-I transmembrane glycoproteins specific to lymphatic endothelial cells, is expressed in various cancers including squamous cell carcinoma (SCC). On the basis of our previous studies, we have developed the hypothesis that PDPN functions in association with the extracellular matrix (ECM) from the cell surface side. The aim of this study was to elucidate the molecular role of PDPN in terms of cell adhesion, proliferation, and migration in oral SCC cells. Forty-four surgical specimens of oral SCC were used for immunohistochemistry for PDPN, and the expression profiles were correlated with their clinicopathological properties. Using ZK-1, a human oral SCC cell system, and five other cell systems, we examined PDPN expression levels by immunofluorescence, western blotting, and real-time PCR. The effects of transient PDPN knockdown by siRNA in ZK-1 were determined for cellular functions in terms of cell proliferation, adhesion, migration, and invasion in association with CD44 and hyaluronan. Cases without PDPN-positive cells were histopathologically classified as less-differentiated SCC, and SCC cells without PDPN more frequently invaded lymphatics. Adhesive properties of ZK-1 were significantly inhibited by siRNA, and PDPN was shown to collaborate with CD44 in cell adhesion to tether SCC cells with hyaluronan-rich ECM of the narrow intercellular space as well as with the stromal ECM. There was no siRNA effect in migration. We have demonstrated the primary function of PDPN in cell adhesion to ECM, which is to secondarily promote oral SCC cell proliferation.


リンク情報
DOI
https://doi.org/10.1038/labinvest.2013.86
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23817087
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000322444300007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/labinvest.2013.86
  • ISSN : 0023-6837
  • PubMed ID : 23817087
  • Web of Science ID : WOS:000322444300007

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