論文

査読有り
2013年10月

Hemophagocytosis-mediated keratinization in oral carcinoma in situ and squamous cell carcinoma: A possible histopathogenesis of keratin pearls

JOURNAL OF CELLULAR PHYSIOLOGY
  • Kamal Al-Eryani
  • ,
  • Jun Cheng
  • ,
  • Tatsuya Abe
  • ,
  • Manabu Yamazaki
  • ,
  • Satoshi Maruyama
  • ,
  • Masayuki Tsuneki
  • ,
  • Ahmed Essa
  • ,
  • Hamzah Babkair
  • ,
  • Takashi Saku

228
10
開始ページ
1977
終了ページ
1988
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/jcp.24364
出版者・発行元
WILEY-BLACKWELL

Although the histopathogenetic process of keratin pearls is still poorly understood, acceleration of keratinization in squamous cell carcinoma (SCC) cells may represent one possible therapeutic avenue. Based on our histopathological observations, we have hypothesized that SCC cells are keratinized by phagocytosis of extravasated erythrocytes. To confirm this hypothesis, we firstly examined immature keratin pearls in oral carcinoma in situ (CIS) and mature ones in SCC by immunohistochemistry. Concentric dyskeratotic cells in CIS keratin pearls became positive for keratin (K) 10, K17, heme oxygenase-1 (HO-1), or protease activated receptor-2 (PAR-2), a candidate regulator for hemophagocytosis. When ZK-1 cells, an SCC cell system, were incubated with human peripheral blood erythrocytes, or with crude and purified hemoglobins (Hbs), their erythro-hemophagocytotic activities were confirmed by immunofluorescence. Immunofluorescence signals for K10, K17, and HO-1 were enhanced due to hemophagocytosis in time-dependent manners. mRNA expression levels for the three molecules were most enhanced by purified Hb, followed by crude Hb and erythrocytes. K17/K10 mRNA expression levels were more elevated when PAR-2 was activated in ZK-1 cells. The results indicated that immature and mature keratin pearls in CIS and SCC were generated by oxidative stresses derived from erythro-hemophagocytosis, which might mediate HO-1 expression and be regulated by PAR-2. Thus, hemorrhage from the rupture of blood vessels can be one of the triggers for keratin pearl formation in oral CIS and SCC. J. Cell. Physiol. 228: 1977-1988, 2013. (c) 2013 Wiley Periodicals, Inc.


リンク情報
DOI
https://doi.org/10.1002/jcp.24364
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23526486
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000320780500004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/jcp.24364
  • ISSN : 0021-9541
  • PubMed ID : 23526486
  • Web of Science ID : WOS:000320780500004

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