論文

査読有り
2012年11月

Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma

HUMAN PATHOLOGY
  • Hamdy Metwaly
  • ,
  • Satoshi Maruyama
  • ,
  • Manabu Yamazaki
  • ,
  • Masayuki Tsuneki
  • ,
  • Tatsuya Abe
  • ,
  • Kai Yu Jen
  • ,
  • Jun Cheng
  • ,
  • Takashi Saku

43
11
開始ページ
1973
終了ページ
1981
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.humpath.2012.02.006
出版者・発行元
W B SAUNDERS CO-ELSEVIER INC

It is poorly understood which cell type, tumor cells, or stromal cells are responsible for the production of extracellular matrix molecules in the neoplastic stroma. We studied the expression of 4 extracellular matrix molecules at the protein and messenger RNA levels in monocellular and 2 kinds of coculture systems between human squamous cell carcinoma (ZK-1) and fibroblast (OF-1) cell lines, which may correspond to carcinoma in situ and squamous cell carcinoma, respectively. Squamous cell carcinoma and carcinoma in situ tissue sections were also investigated by immunohistochemistry and in situ hybridization for extracellular matrix. Immunohistochemically, perlecan and tenascin C were localized in carcinoma cells in carcinoma in situ, whereas they were in the stromal space in squamous cell carcinoma. In monocellular culture conditions, expression levels for perlecan, tenascin C, and laminin were more predominant in ZK-1 than in OF-1, although those for fibronectin were more enhanced in OF-1. However, these extracellular matrix expression levels of OF-I were elevated, whereas those of ZK-1 dropped when they were in coculture conditions. The differences between ZK-1 and OF-I were significantly more evident in direct contact (ZK-1/OF-1, 56%-22%) than in indirect contact (63%-39%). These results indicate that oral squamous cell carcinoma cells produce extracellular matrix in the absence of stromal fibroblasts (or in carcinoma in situ) and that they stop producing extracellular matrix in the presence of fibroblasts (or in squamous cell carcinoma). It is hence suggested that stromal fibroblasts after direct contact with invading squamous cell carcinoma cells are more responsible than squamous cell carcinoma cells for the formation of neoplastic stroma, whereas carcinoma in situ cells have to produce and deposit extracellular matrix by themselves to form intraepithelial microstromal spaces. (C) 2012 Elsevier Inc. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.humpath.2012.02.006
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22575259
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000310654500023&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.humpath.2012.02.006
  • ISSN : 0046-8177
  • PubMed ID : 22575259
  • Web of Science ID : WOS:000310654500023

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