論文

査読有り
2012年6月

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

MODERN PATHOLOGY
  • Hiroko Ida-Yonemochi
  • ,
  • Satoshi Maruyama
  • ,
  • Takanori Kobayashi
  • ,
  • Manabu Yamazaki
  • ,
  • Jun Cheng
  • ,
  • Takashi Saku

25
6
開始ページ
784
終了ページ
794
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/modpathol.2011.218
出版者・発行元
NATURE PUBLISHING GROUP

Immunohistochemical loss of keratin (K)13 is one of the most valuable diagnostic criteria for discriminating carcinoma in situ (CIS) from non-malignancies in the oral mucosa while K13 is stably immunolocalized in the prickle cells of normal oral epithelium. To elucidate the molecular mechanism for the loss of K13, we compared the immunohistochemical profiles for K13 and K16 which is not expressed in normal epithelia, but instead enhanced in CIS, with their mRNA levels by in-situ hybridization in formalin-fixed paraffin sections prepared from 23 CIS cases of the tongue, which were surgically removed. Reverse transcriptase-PCR was also performed using RNA samples extracted from laser-microdissected epithelial fragments of the serial paraffin sections in seven of the cases. Although more enhanced expression levels for K16 were confirmed at both the protein and gene levels in CIS in these seven cases, the loss of K13 was associated with repressed mRNA levels in four cases, but not in the other three cases. The results suggest that the loss of K13 is partly due to its gene repression, but may also be due to some unknown post-translational events. Modern Pathology (2012) 25, 784-794; doi:10.1038/modpathol.2011.218; published online 3 February 2012


リンク情報
DOI
https://doi.org/10.1038/modpathol.2011.218
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22301701
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000304839300002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/modpathol.2011.218
  • ISSN : 0893-3952
  • PubMed ID : 22301701
  • Web of Science ID : WOS:000304839300002

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