論文

査読有り
2011年3月

Acetic Acid Treatment for Wrinkle-Free Oral Mucosal Epithelia in Paraffin Section Preparation

MICROSCOPY RESEARCH AND TECHNIQUE
  • Md. Shahidul Ahsan
  • ,
  • Satoshi Maruyama
  • ,
  • Jun Cheng
  • ,
  • Kamal Al-Eryani
  • ,
  • Manabu Yamazaki
  • ,
  • Mayumi Hasegawa
  • ,
  • Masayuki Tsuneki
  • ,
  • Takashi Saku

74
3
開始ページ
264
終了ページ
268
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/jemt.20900
出版者・発行元
WILEY-BLACKWELL

For histopathological assessment of oral borderline malignancies, it is important to carefully detect subtle epithelial changes on fully stretched tissue sections. However, it is not generally easy to obtain wrinkle-free sections when using formalin-fixed paraffin-embedded oral mucosal samples. Since acetic acid treatment is already utilized for large brain tissue sections, we examined whether that treatment was also effective for oral mucosal tissues containing normal to malignant epithelial lesions. Paraffin sections were floated in various concentrations of acetic acid for 10 min after stretching in water for 1 min, then wrinkle formations were examined using hematoxylin and eosin staining, as well as for staining intensity with keratin immunohistochemistry. Wrinkles were formed in both epithelial and connective tissue zones of sections treated with less than a 40-mM (0.25%) concentration of acetic acid. In contrast, treatments with concentrations at 80 mM (0.5%) and higher resulted in cracking between the epithelial layer and lamina propria, as well as poor immunohistochemical results for keratins 13 and 17, even though the wrinkles completely disappeared. These results indicate that 40 mM is the optimal concentration of acetic acid solution to prevent wrinkles in the epithelial layer while maintaining the immunohistochemical qualities of oral mucosa tissue sections, especially those containing borderline malignant epithelial lesions. Microsc. Res. Tech. 74: 264-268,2011. (C) 2010 Wiley-Liss, Inc.


リンク情報
DOI
https://doi.org/10.1002/jemt.20900
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20623754
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000288095200008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/jemt.20900
  • ISSN : 1059-910X
  • PubMed ID : 20623754
  • Web of Science ID : WOS:000288095200008

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