エスプルガーウィルフレッド (Wilfred Espulgar) - マイポータル

基本情報

所属: 大阪大学産業科学研究所特任助教（常勤）

学位: 博士(大阪大学)

連絡先: wilfredap.eng.osaka-u.ac.jp
研究者番号: 40773099
ORCID ID: https://orcid.org/0000-0002-8765-655X
J-GLOBAL ID: 202001020086432347
researchmap会員ID: R000011685

研究キーワード

研究分野

経歴

2020年4月 - 現在

大阪大学, 産業科学研究所
2018年10月 - 2020年3月

大阪大学, 大学院工学研究科
2015年10月 - 2018年9月

大阪大学, 大学院工学研究科
2010年10月 - 2012年3月

De La Salle University, College of Science (Lecturer I)
2007年5月 - 2009年3月

Saint Joseph’s Academy, High School Department (Science Teacher)

学歴

2012年4月 - 2015年9月

大阪大学, 大学院工学研究科
2008年5月 - 2010年12月

De La Salle University, Physics, MS Physics
2003年6月 - 2007年3月

Philippine Normal University, Physical Sciences, BS Physics for Teachers

論文

EIS and Amperometric Analysis of Gold Nanoparticle Probes on the Immunosensor Surface

Shuto Osaki, Wilfred Villariza Espulgar, Masato Saito, Eiichi Tamiya

ECS Meeting Abstracts MA2023-01(48) 2532-2532 2023年8月28日
Development of Nano–Micro Fused LSPR Chip for In Situ Single-Cell Secretion Analysis

Yuhei Terada, Ain Obara, Jonathan Campos Briones, Xi Luo, Wilfred Villariza Espulgar, Masato Saito, Hyota Takamatsu, Eiichi Tamiya

Micromachines 14(7) 1404-1404 2023年7月11日
Linking antigen specific T-cell dynamics in a microfluidic chip to single cell transcription patterns.

Hiroki Ide, Taiki Aoshi, Masato Saito, Wilfred Villariza Espulgar, Jonathan Campos Briones, Masahito Hosokawa, Hiroko Matsunaga, Koji Arikawa, Haruko Takeyama, Shohei Koyama …

Biochemical and biophysical research communications 657 8-15 2023年3月15日
Optimization of electrochemical analysis for signal amplification in gold nanoparticle-probed immunoassays

Shuto Osaki, Wilfred Villariza Espulgar, Shin-ichi Wakida, Masato Saito, Eiichi Tamiya

Electrochimica Acta 141180-141180 2022年9月
The future of microfluidics in immune checkpoint blockade

Jonathan Briones, Wilfred Espulgar, Shohei Koyama, Hyota Takamatsu, Eiichi Tamiya, Masato Saito

Cancer Gene Therapy 28(9) 895-910 2021年9月
A design and optimization of a high throughput valve based microfluidic device for single cell compartmentalization and analysis.

Jonathan Briones, Wilfred Espulgar, Shohei Koyama, Hyota Takamatsu, Eiichi Tamiya, Masato Saito

Scientific reports 11(1) 12995-12995 2021年6月21日
Profiling T cell interaction and activation through microfluidics-assisted serial encounter with APCs

Hiroki Ide, Wilfred Villariza Espulgar, Masato Saito, Taiki Aoshi, Shohei Koyama, Hyota Takamatsu, Eiichi Tamiya

Sensors and Actuators B: Chemical 330 129306-129306 2021年3月
Deskilled and Rapid Drug-Resistant Gene Detection by Centrifugal Force-Assisted Thermal Convection PCR Device

Wilfred Villariza Espulgar, Masato Saito, Kazuya Takahashi, Sakiko Ushiro, Norihisa Yamamoto, Yukihiro Akeda, Shigeto Hamaguchi, Kazunori Tomono, Eiichi Tamiya

Sensors 21(4) 1225-1225 2021年2月9日
Cauliflower-Like Nanostructured Localized Surface Plasmon Resonance Biosensor Chip for Cytokine Detection

Xi Luo, Chen Zhu, Masato Saito, Wilfred Villariza Espulgar, Xiaoming Dou, Yuhei Terada, Ain Obara, Sachiyo Uchiyama, Eiichi Tamiya

Bulletin of the Chemical Society of Japan 93(9) 1121-1126 2020年9月15日査読有り
SERS Active Hierarchical Nanopillar-huddle Array Fabricated via the Combination of Nanoimprint Lithography and Anodization

Shu JIANG, Wilfred V. ESPULGAR, Xi LUO, Masato SAITO, Hiroyuki YOSHIKAWA, Eiichi TAMIYA

Electrochemistry 88(3) 165-173 2020年5月5日査読有り
A Microfluidic Platform for Single Cell Fluorometric Granzyme B Profiling

Jonathan C. Briones, Wilfred V. Espulgar, Shohei Koyama, Hiroyuki Yoshikawa, JeongHoon Park, Yujiro Naito, Atsushi Kumanogoh, Eiichi Tamiya, Hyota Takamatsu, Masato Saito

Theranostics 10(1) 123-132 2020年査読有り
Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

Chen Zhu, Xi Luo, Wilfred Villariza Espulgar, Shohei Koyama, Atsushi Kumanogoh, Masato Saito, Hyota Takamatsu, Eiichi Tamiya

MICROMACHINES 11(1) 2020年1月査読有り
Single Cell Analysis of Neutrophils NETs by Microscopic LSPR Imaging System

Riyaz Ahmad Mohamed Ali, Daiki Mita, Wilfred Espulgar, Masato Saito, Masayuki Nishide, Hyota Takamatsu, Hiroyuki Yoshikawa, Eiichi Tamiya

Micromachines 11(1) 52-52 2019年12月31日査読有り
Utility of centrifugation-controlled Convective (C3) Flow for Rapid On-chip ELISA

Wilfred Espulgar, Tatsuro Tadokoro, Eiichi Tamiya, Masato Saito

9, Article number: 20150 (2019) 9(20150) 2019年12月査読有り筆頭著者
Single Cell Receptor Analysis Aided by a Centrifugal Microfluidic Device for Immune Cells Profiling

Chen Zhu, Wilfred Villariza Espulgar, Woosik Yoo, Shohei Koyama, Xiaoming Dou, Atsushi Kumanogoh, Eiichi Tamiya, Hyota Takamatsu, Masato Saito

Bulletin of the Chemical Society of Japan 92(11) 1834-1839 2019年11月15日査読有り
In vitro single-cell visuallization and profiling of t cell-antigen presenting cell (APC) interaction

Hiroki Ide, Wilfred Villariza Espulgar, Masato Saito, Taiki Aoshi, Eiichi Tamiya

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 480-481 2019年
Electrochemically Modulated Surface-Enhanced Raman Spectra of Aminoglutethimide (AGI) on a Ag-Sputtered Electrode

Zicheng Zhu, Wilfred Villariza Espulgar, Hiroyuki Yoshikawa, Masato Saito, Bin Fan, Xiaoming Dou, Eiichi Tamiya

Bulletin of the Chemical Society of Japan 91(11) 1579-1585 2018年11月15日査読有り
Electrochemiluminescence-based Monitoring of Activated Human Neutrophils Using Luminol Derivative Immobilized onto Screen-printed Electrodes

Joyotu Mazumder, Yuka Kizawa, Wilfred Espulgar, Hiroyuki Yoshikawa, Masato Saito, Shouhei Koyama, Hyota Takamatsu, Atsushi Kumanogoh, Eiichi Tamiya

Chemistry Letters 47(10) 1337-1340 2018年10月5日査読有り
Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

Masato Saito, Natsuko Uchida, Shunsuke Furutani, Mizuho Murahashi, Wilfred Espulgar, Naoki Nagatani, Hidenori Nagai, Yuki Inoue, Tomohiko Ikeuchi, Satoshi Kondo …

Microsystems & Nanoengineering 4(1) 2018年4月査読有り
One-step nanoimprinted hybrid micro-/nano-structure for in situ protein detection of isolated cell array via localized surface plasmon resonance

Riyaz Ahmad Mohamed Ali, Wilfred Villariza Espulgar, Wataru Aoki, Shu Jiang, Masato Saito, Mitsuyoshi Ueda, Eiichi Tamiya

Japanese Journal of Applied Physics 57(3S2) 03EC03-03EC03 2018年3月1日査読有り

もっとみる

講演・口頭発表等

Rapid on-chip ELISA aided by centrifugation-assisted thermal convection reflow

Espulgar, W

38th SPP Physics Conference | Online 招待有り
1細胞分泌因子の分離分析に向けた遠心操作型マイクロ流路チップの検討

第14回バイオ関連化学シンポジウム
Towards centrifugation-assisted cell trapping and isolation in a two-phase liquid

Espulgar, W, Saito, M, Koyama, S. Kumanogoh A, Takamatsu H, Tamiya, E

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS)
Modified centrifugal microfluidic chip system with reflow capability for single cell preparation

Espulgar, W, Saito, M, Koyama S, Takamatsu H, Tamiya, E

80th JSAP Autumn Meeting
Induced forward and reverse flow in a centrifugal chip device utilized for efficient single cell preparation

Espulgar, W, Saito, M, Koyama S, Takamatsu H, Tamiya, E

10th International Conference on Molecular Electronics & BioElectronics
A centrifugal microfluidic chip system enabling efficient single cell trapping by reflow

Espulgar, W, Saito, M, Koyama S, Takamatsu H, Tamiya, E

12th International Workshop on Approaches to Single Cell Analysis
Automated reflow system controlled by reverse rotation of a rotating stage for single cell trapping

Espulgar, W, Saito, M, Tamiya, E

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS)
Automated reverse rotation centrifugal microfluidic chip system for reflow and trapping of single cells

Espulgar, W, Saito, M, Koyama S, Takamatsu H, Tamiya, E

79th JSAP Autumn Meeting
“Microfluidic devices for cell isolation and for surface receptor profiling of single white blood cells

Espulgar, W.V, Ono, T, Chen, Z, Saito, M, Koyama S, Takamatsu H, Tamiya, E

Biosensors 2018: 28th Anniversary World Congress on Biosensors
Cell isolation and surface receptor profiling platform for single leukocytes

Espulgar, W, Ono, T, Chen, Z, Saito, M, Koyama S, Takamatsu H, Tamiya, E

The 98th Chemical Society of Japan (CSJ) Annual Meeting
Single cell preparation in a centrifugal microfluidic chip system with reflow capability

Espulgar, W, Saito, M, Koyama S, Takamatsu H, Tamiya, E

66th JSAP Spring Meeting
Automated field-deployable biological and chemical warfare agents sensing system: integration of aerosol sampling and biosensing technologies

Espulgar, W. V, Saito, M, Uchida, N, Furutani, S, Murahashi, M, Nagatani, N, Nagai, H, Inoue, Y, Ikeuchi, T, Kondo, S, Uzawa, H, Seto, Y, Tamiya, E

AsiaSense2018
Single cell surface receptor analysis in the designed microfluidic device

Espulgar, W, Okajima, T, Takamatsu H, Aoki W, Saito, M, Kumanogoh A, Ueda M, Tamiya, E

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS)
Evaluation of extracellular matrix influence to beat synchronicity of iPSC-derived cardiomyocyte-NHC fibroblast 3D tissues using image correlation analysis

Espulgar, W, Shima, F, Hiura A, Takamura Y, Fukumoto K, Akagi T, Akashi M, Tamiya, E

78th JSAP Autumn Meeting
Centrifugation assisted platforms for cell surface receptor profiling and for thermal convective PCR

Espulgar, W, Saito, M, Tamiya, E

Microfluidic Compartmentalization Workshop “Fundamentals and Applications of Microfluidic Compartmentalization 招待有り
ConnecTech : An online platform to promote and facilitate technology transfer between academic institutions and corporations

Espulgar, W, Ansari, S. T, Navarro V.B, Ghadban, C, Potin L

Gap Summit 2017
Cell sorting efficiency of weir structure-based filters in the designed microfluidic chip

Espulgar, W, Kizawa Y, Okajima, T, Saito, M, Tamiya, E

The 97th Chemical Society of Japan (CSJ) Annual Meeting
Towards single-cell level pharmacological study with the aid of microfluidics

Espulgar, W, Okajima T, Aoki, W, Saito, M, Lee, J. K, Ueda M, Tamiya, E

1st International & 7th Annual Convention and Scientific Meeting 招待有り
Beat motion kinetics of neonatal rat cardiomyocytes trapped in a centrifugal microfluidic chip: evaluated with video analysis and Raman imaging

Espulgar, W, Saito, M, Lee, J. K, Tamiya, E

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS)
Workshop on microfluidics for cell study

Espulgar, W

1st Biosensing Technology Workshop & Conference 招待有り

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