2018年3月1日
One-step nanoimprinted hybrid micro-/nano-structure for in situ protein detection of isolated cell array via localized surface plasmon resonance
Japanese Journal of Applied Physics
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- 巻
- 57
- 号
- 3S2
- 開始ページ
- 03EC03
- 終了ページ
- 03EC03
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.7567/jjap.57.03ec03
- 出版者・発行元
- IOP Publishing
Nanoplasmonic biosensors show high potentials as label-free devices for continuous monitoring in biomolecular analyses. However, most current sensors comprise multiple-dedicated layers with complicated fabrication procedures, which increases production time and manufacturing costs. In this work, we report the synergistic integration of cell-trapping microwell structures with plasmonic sensing nanopillar structures in a single-layered substrate by one-step thermal nanoimprinting. Here, microwell arrays are used for isolating cells, wherein gold-capped nanostructures sense changes in local refractive index via localized surface plasmon resonance (LSPR). Hence, proteins secreted from trapped cells can be label-freely detected as peak shifts in absorbance spectra. The fabricated device showed a detection limit of 10 ng/mu L anti-IgA. In Pichia pastoris cells trial analysis, a red shift of 6.9 nm was observed over 12 h, which is likely due to the protein secretion from the cells. This approach provides an inexpensive, rapid, and reproducible alternative for mass production of biosensors for continuous biomolecular analyses. (C) 2018 The Japan Society of Applied Physics
- リンク情報
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- DOI
- https://doi.org/10.7567/jjap.57.03ec03
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000425737100014&DestApp=WOS_CPL
- URL
- http://stacks.iop.org/1347-4065/57/i=3S2/a=03EC03?key=crossref.3680846d92aec61abdbaae6fe1da73f2
- URL
- http://stacks.iop.org/1347-4065/57/i=3S2/a=03EC03/pdf
- ID情報
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- DOI : 10.7567/jjap.57.03ec03
- ISSN : 0021-4922
- eISSN : 1347-4065
- Web of Science ID : WOS:000425737100014