論文

査読有り 筆頭著者 国際誌
2020年4月6日

The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties.

Scientific reports
  • Kentaro Oniki
  • Hirofumi Nohara
  • Ryunosuke Nakashima
  • Yui Obata
  • Narumi Muto
  • Yuki Sakamoto
  • Keiko Ueno-Shuto
  • Tadashi Imafuku
  • Yu Ishima
  • Hiroshi Watanabe
  • Toru Maruyama
  • Koji Otake
  • Yasuhiro Ogata
  • Mary Ann Suico
  • Hirofumi Kai
  • Tsuyoshi Shuto
  • Junji Saruwatari
  • 全て表示

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開始ページ
5973
終了ページ
5973
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-020-62872-5

Oxidative stress and inflammation play a key role in the age-related decline in the respiratory function. Adipokine in relation to the metabolic and inflammatory systems is attracting growing interest in the field of respiratory dysfunction. The present clinical and experimental studies investigated the role of the disulfide bond-forming oxidoreductase A-like protein (DsbA-L) gene, which has antioxidant and adiponectin multimeric (i.e. activation) properties, on the respiratory function of the elderly. We performed a retrospective longitudinal genotype-phenotype relationship analysis of 318 Japanese relatively elderly participants (mean age ± standard deviation: 67.0 ± 5.8 years) during a health screening program and an in vitro DsbA-L knock-down evaluation using 16HBE14o-cells, a commonly evaluated human airway epithelial cell line. The DsbA-L rs1917760 polymorphism was associated with a reduction in the ratio of forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) and %FEV1 and with the elevation of the prevalence of FEV1/FVC < 70%. We also confirmed that the polymorphism was associated with a decreased respiratory function in relation to a decrease in the ratio of high-molecular-weight adiponectin/total adiponectin (as a marker of adiponectin multimerization) and an increase in the oxidized human serum albumin (as an oxidative stress marker). Furthermore, we clarified that DsbA-L knock-down induced oxidative stress and up-regulated the mucus production in human airway epithelial cells. These findings suggest that the DsbA-L gene may play a role in protecting the respiratory function of the elderly, possibly via increased systemic adiponectin functions secreted from adipocytes or through systemic and/or local pulmonary antioxidant properties.

リンク情報
DOI
https://doi.org/10.1038/s41598-020-62872-5
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32249844
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136289
ID情報
  • DOI : 10.1038/s41598-020-62872-5
  • PubMed ID : 32249844
  • PubMed Central 記事ID : PMC7136289

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