論文

査読有り
2017年12月

Fibroblasts promote the collective invasion of ameloblastoma tumor cells in a 3D coculture model

FEBS OPEN BIO
  • Takao Fuchigami
  • ,
  • Hirofumi Koyama
  • ,
  • Michiko Kishida
  • ,
  • Yoshiaki Nishizawa
  • ,
  • Mikio Iijima
  • ,
  • Toshiro Kibe
  • ,
  • Masahiro Ueda
  • ,
  • Tohru Kiyono
  • ,
  • Yoshimasa Maniwa
  • ,
  • Norifumi Nakamura
  • ,
  • Shosei Kishida

7
12
開始ページ
2000
終了ページ
2007
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/2211-5463.12313
出版者・発行元
WILEY

Ameloblastoma is a benign tumor of the odontogenic epithelium with several histological subtypes. All subtypes of ameloblastoma contain abundant stroma; the tumor cells invade collectively into the surrounding tissues without losing intratumor cell attachments. However, the molecular mechanisms mediating ameloblastoma invasion remain unclear. Here, we evaluated the functional significance of the interactions between ameloblastoma tumor cells and stromal fibroblasts on collective cellular invasion using a three-dimensional cultivation method, double-layered collagen gel hemisphere (DL-CGH) culture. The AM-1 plexiform and AM-3 follicular human ameloblastoma cell lines and HFF-2 human fibroblasts were labeled with GFP and DsRed, respectively. Collective cellular invasion of ameloblastoma cells was assessed in the presence or absence of fibroblasts. Notably, without fibroblasts, AM-1 cells formed sharp, plexiform-like invasive processes, whereas AM-3 cells formed a series of blunt processes often observed during collective migration. In comparison, under the cocultures with HFF-2 fibroblasts, AM-3 cells formed tuft-like invasive processes and collectively invaded into outer layer more than that observed with AM-1 cells. Moreover, HFF-2 fibroblasts localized to the tips of the invasive tumor processes. These findings suggest that tumor-associated cells assist tumor cell invasion. Microscopic analysis of sectioned three-dimensional cultures revealed that AM-3/HFF-2 hemispheres were histologically similar to follicular ameloblastoma tumor samples. Therefore, our findings suggest that ameloblastoma subtypes exhibit distinct invasion patterns and that fibroblasts promote collective tumor invasion in follicular ameloblastoma.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1002/2211-5463.12313
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29226086
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000417109000016&DestApp=WOS_CPL

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