論文

査読有り
2016年1月

Microfluidic Approaches for Protein Crystal Structure Analysis

ANALYTICAL SCIENCES
  • Masatoshi Maeki
  • ,
  • Hiroshi Yamaguchi
  • ,
  • Manabu Tokeshi
  • ,
  • Masaya Miyazaki

32
1
開始ページ
3
終了ページ
9
記述言語
英語
掲載種別
DOI
10.2116/analsci.32.3
出版者・発行元
JAPAN SOC ANALYTICAL CHEMISTRY

This review summarizes two microfluidic-based protein crystallization methods, protein crystallization behavior in the microfluidic devices, and their applications for X-ray crystal structure analysis. Microfluidic devices provide many advantages for protein crystallography; they require small sample volumes, provide high-throughput screening, and allow control of the protein crystallization. A droplet-based protein crystallization method is a useful technique for high-throughput screening and the formation of a single crystal without any complicated device fabrication process. Well-based microfluidic platforms also enable effective protein crystallization. This review also summarizes the protein crystal growth behavior in microfluidic devices as, is known from viewpoints of theoretical and experimental approaches. Finally, we introduce applications of microfluidic devices for on-chip crystal structure analysis.

Web of Science ® 被引用回数 : 33

リンク情報
DOI
https://doi.org/10.2116/analsci.32.3
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201602201732598840
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000368963300002&DestApp=WOS_CPL
URL
http://jglobal.jst.go.jp/public/201602201732598840

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