論文

査読有り
2015年1月

Microfluidic chips with multi-junctions: an advanced tool in recovering proteins from inclusion bodies

BIOENGINEERED
  • Hiroshi Yamaguchi
  • ,
  • Masaya Miyazaki

6
1
開始ページ
1
終了ページ
4
記述言語
英語
掲載種別
DOI
10.4161/21655979.2014.987022
出版者・発行元
TAYLOR & FRANCIS INC

Active recombinant proteins are used for studying the biological functions of genes and for the development of therapeutic drugs. Overexpression of recombinant proteins in bacteria often results in the formation of inclusion bodies, which are protein aggregates with non-native conformations. Protein refolding is an important process for obtaining active recombinant proteins from inclusion bodies. However, the conventional refolding method of dialysis or dilution is time-consuming and recovered active protein yields are often low, and a cumbersome trial-and-error process is required to achieve success. To circumvent these difficulties, we used controllable diffusion through laminar flow in microchannels to regulate the denaturant concentration. This method largely aims at reducing protein aggregation during the refolding procedure. This Commentary introduces the principles of the protein refolding method using microfluidic chips and the advantage of our results as a tool for rapid and efficient recovery of active recombinant proteins from inclusion bodies.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.4161/21655979.2014.987022
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000351456500001&DestApp=WOS_CPL

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