論文

査読有り
2014年9月

Single Nucleotide Polymorphism Typing with a Surface Plasmon Resonance-Based Sensor Using Hybridization Enhancement Blockers

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Shiro Okumura
  • ,
  • Rieko Kuroda
  • ,
  • Kuniyo Inouye

174
2
開始ページ
494
終了ページ
505
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s12010-014-1072-2
出版者・発行元
HUMANA PRESS INC

We constructed a simple method for discrimination of single nucleotide polymorphism (SNP) with a surface plasmon resonance (SPR)-based sensor by determining the binding volume (BV) between a target SNP allele and a probe complementary to the target. In the method, a novel additive termed "blocker," which is a short single-stranded DNA complementary to the target, was used. The blocker enhanced the BV of the target only to the full-match probe 10-fold or more, so the SNP alleles could be discriminated readily. The effect of the blocker concentration was also examined. The BV to only a full-match probe increased with increasing the blocker concentration and reached a plateau at the concentration of 300-500 nM. To assess the effectiveness of this method, the SNP associated with progressive rod-cone degeneration in dog was determined. The results of genotyping with the method were in good agreement with those obtained by direct sequencing.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1007/s12010-014-1072-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25082764
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000341443900004&DestApp=WOS_CPL

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