- HUMANA PRESS INC
We constructed a simple method for discrimination of single nucleotide polymorphism (SNP) with a surface plasmon resonance (SPR)-based sensor by determining the binding volume (BV) between a target SNP allele and a probe complementary to the target. In the method, a novel additive termed "blocker," which is a short single-stranded DNA complementary to the target, was used. The blocker enhanced the BV of the target only to the full-match probe 10-fold or more, so the SNP alleles could be discriminated readily. The effect of the blocker concentration was also examined. The BV to only a full-match probe increased with increasing the blocker concentration and reached a plateau at the concentration of 300-500 nM. To assess the effectiveness of this method, the SNP associated with progressive rod-cone degeneration in dog was determined. The results of genotyping with the method were in good agreement with those obtained by direct sequencing.
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