論文

査読有り
2014年7月

Toxicity of Parasporin-4 and Health Effects of Pro-parasporin-4 Diet in Mice

TOXINS
  • Shiro Okumura
  • ,
  • Hironori Koga
  • ,
  • Kuniyo Inouye
  • ,
  • Eiichi Mizuki

6
7
開始ページ
2115
終了ページ
2126
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/toxins6072115
出版者・発行元
MDPI AG

Parasporin-4 (PS4) is an aerolysin-type beta-pore-forming toxin produced by Bacillus thuringiensis strain A1470. It exhibits specific cytotoxicity against human cancer cell lines; therefore, it is expected to be useful for the diagnosis and treatment of particular types of cancer cells. We examined the acute toxicity of PS4 on ICR mice. The LD50 value was 160 mu g/kg by a subcutaneous route. Potassium, ammonium, magnesium ion, creatinine, and urea nitrogen decreased in urine by the injection of PS4. Simultaneously, creatinine and urea nitrogen in mice serum increased. These results imply that PS4 impairs kidney function in mice. PS4 is obtained from Pro-parasporin-4 (ProPS4) by processing, and ProPS4 is produced by recombinant Escherichia coli as the inclusion body. The inclusion body of ProPS4 can be solubilized in a weak acid solution and activated by pepsin, implying that it would be solubilized and activated in the stomach of mammals after oral administration. Thus, the influence of the oral administration of it by C57BL/6J mice was examined. Although ProPS4 was activated to PS4 in the mouse digestive tract, any serious health hazard was not observed and there was no significant difference in body weight change.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.3390/toxins6072115
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25033273
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000340039400011&DestApp=WOS_CPL

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