BACKGROUND: Visual information conveyed through the extrageniculate visual pathway, which runs from the retina via the superior colliculus (SC) and the lateral posterior nucleus (LPN) of the thalamus to the higher visual cortex, plays a critical role in the visual capabilities of many mammalian species. However, its functional role in the higher visual cortex remains unclear. Here, we observed visual cortical area activity in anesthetized mice to evaluate the role of the extrageniculate pathway on their specialized visual properties. RESULTS: The preferred stimulus velocities of neurons in the higher visual areas (lateromedial [LM], anterolateral [AL], anteromedial [AM], and rostrolateral [RL] areas) were measured using flavoprotein fluorescence imaging and two-photon calcium imaging and were higher than those in the primary visual cortex (V1). Further, the velocity-tuning properties of the higher visual areas were different from each other. The response activities in these areas decreased after V1 ablation; however, the visual properties' differences were preserved. After SC destruction, these preferences for high velocities disappeared, and their tuning profiles became similar