The purpose of this study was to construct an organizational climate scale for use in the nursing setting and examine the relationship between the organizational climate, burnout, and job situation. Staff nurses (n=151) employed at a hospital were administered the Japanese version of the Maslach Burnout Inventory for measuring burnout, a scale for measuring organizational climate and a scale for measuring job situations. Factor analysis identified five factors in the organizational climate scale: Positive climate, Authoritative climate, Support from matron, Burden on business, and Good communication. These factors, with the exception of the Authoritative climate, had fixed reliability levels. The correlation analysis between the organizational climate scores and job situation scores revealed that there were significant correlations among all the job situation variables and the Burden on business. In addition, multiple regression analysis revealed that emotional exhaustion could be explained by Good communication, Burden on business, and role ambiguity. Depersonalization could be explained by Positive climate and the Burden on business. However, accomplishments could not be explained by either organizational climate or job situation.