論文

査読有り 国際誌
2015年2月6日

Identification of a novel cell-penetrating peptide targeting human glioblastoma cell lines as a cancer-homing transporter.

Biochemical and biophysical research communications
  • Moritoshi Higa
  • ,
  • Chiaki Katagiri
  • ,
  • Chigusa Shimizu-Okabe
  • ,
  • Tomoyuki Tsumuraya
  • ,
  • Masanori Sunagawa
  • ,
  • Mariko Nakamura
  • ,
  • Shogo Ishiuchi
  • ,
  • Chitoshi Takayama
  • ,
  • Eisaku Kondo
  • ,
  • Masayuki Matsushita

457
2
開始ページ
206
終了ページ
12
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2014.12.089

Cell-penetrating peptides (CPPs) as a novel biomedical delivery system have been highly anticipated, since they can translocate across biological membranes and are capable of transporting their cargo inside live cells with minimal invasiveness. However, non-selective internalization in various cell types remains a challenge in the clinical application of CPPs, especially in cancer treatment. In this study, we attempted to identify novel cancer-homing CPPs to target glioblastoma multiforme (GBM), which is often refractory and resistant to treatment. We screened for CPPs showing affinity for the human GBM cell line, U87MG, from an mRNA display random peptide library. One of the candidate peptides which amino-acid sequence was obtained from the screening showed selective cell-penetrating activity in U87MG cells. Conjugation of the p16(INK4a) functional peptide to the GBM-selective CPP induced cellular apoptosis and reduced phosphorylated retinoblastoma protein levels. This indicates that the CPP was capable of delivering a therapeutic molecule into U87MG cells inducing apoptosis. These results suggest that the novel CPP identified in this study permeates with high affinity into GBM cells, revealing it to be a promising imaging and therapeutic tool in the treatment of glioblastoma.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2014.12.089
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25562654
ID情報
  • DOI : 10.1016/j.bbrc.2014.12.089
  • PubMed ID : 25562654

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