論文

2008年3月

Conditionally positive effect of the TetR-family transcriptional regulator AtrA on streptomycin production by Streptomyces griseus

MICROBIOLOGY-SGM
  • Setsu Hirano
  • ,
  • Katsuyuki Tanaka
  • ,
  • Yasuo Ohnishi
  • ,
  • Sueharu Horinouchi

154
Pt 3
開始ページ
905
終了ページ
914
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1099/mic.0.2007/014381-0
出版者・発行元
SOC GENERAL MICROBIOLOGY

AtrA, a transcriptional activator for actII-ORF4, encoding the pathway-specific transcriptional activator of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor A3(2), has been shown to bind the region upstream from the promoter of strR, encoding the pathway-specific transcriptional activator of the streptomycin biosynthetic gene cluster in Streptomyces griseus [Uguru et al. (2005) Mol Microbiol 58, 131-150]. The atrA orthologue (atrA-g) in S. griseus was constitutively transcribed throughout growth from a promoter located about 250 nt upstream of the translational start codon, as determined by S1 nuclease mapping. DNase I footprinting showed that histidine-tagged AtrA-g bound an inverted repeat located upstream of strR at positions -117 to -142 relative to the transcriptional start point of strR as +1. This AtrA-g-binding site was between two AdpA-binding sites at approximately nucleotide positions -270 and -50. AdpA is a central transcriptional activator in the A-factor regulatory cascade and essential for the transcription of strR. AtrA-g and AdpA simultaneously bound the respective binding sites. In contrast to AdpA, AtrA-g was non-essential for strR transcription; an atrA-g-disrupted strain produced streptomycin on routine agar media to the same extent as the wild-type strain. However, the atrA-g-disrupted strain tended to produce a smaller amount of streptomycin than the wild-type strain under some conditions, for example, on Bennett agar containing 1 % maltose and on a minimal medium. Therefore, AtrA-g had a conditionally positive effect on streptomycin production, as a tuner, probably by enhancing the AdpA-dependent transcriptional activation of strR in a still unknown manner.

リンク情報
DOI
https://doi.org/10.1099/mic.0.2007/014381-0
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254451500022&DestApp=WOS_CPL
URL
http://www.ncbi.nlm.nih.gov/pubmed/18310036
ID情報
  • DOI : 10.1099/mic.0.2007/014381-0
  • ISSN : 1350-0872
  • Web of Science ID : WOS:000254451500022

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