Full Professor, Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine
Sep 2002
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Mar 2011
Full Professor, Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University School of Medicine
Jul 1998
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Aug 2002
Associate Professor, Department of Pathology, Cancer Research Institute, Sapporo Medical College
1993
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1998
Assistant Professor, Department of Pathology, Cancer Research Institute, Sapporo Medical College
Nov 1990
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Sep 1993
Instructor, Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine
Okita K, Mizuguchi T, Shigenori O, Ishii M, Nishidate T, Ueki T, Meguro M, Kimura Y, Tanimizu N, Ichinohe N, Torigoe T, Kojima T, Mitaka T, Sato N, Sawada N, Hirata K
Shibata C, Mizuguchi T, Kikkawa Y, Nobuoka T, Oshima H, Kawasaki H, Kawamoto M, Katsuramaki T, Mitaka T, Hirata K
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 12 78-87 Jan 2006 [Refereed]
Mizuguchi T, Oshima H, Imaizumi H, Kohara H, Kawamoto M, Nobuoka T, Kawasaki H, Harada K, Masuda Y, Kikkawa Y, Mitaka T, Asai Y, Hirata K
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 13 551-557 Nov 2005 [Refereed]
A small hepatocyte can clonally proliferate for more than 3 months when the cells are cultured in DMEM supplemented with 10% FBS,10mM nicotinamide, 1mM ascorbic acid 2-phosphate, 10ng/mI EGF and 1% DMSO.The cell divides to form a colony and the nu...
A.Sepsis and Cholestasis---Basic findings in the sinusoid and in bile canaliculus---Hepatic dysfunction, as one of unfavorable the pathophysiologic condition in sepsis, is induced by the alterations in both macro- and microcirculatory disturbance ...
We isolated a kind of hepatic progenitor cells, small hepatocytes, from an adult rat liver. A small hepatocyte can clonally proliferate for more than 3 months when the cells are cultured in DMEM supplemented with 10% FBS, 10mM nicotinamide, 1mM as...
Small hepatocytes (SHs), which are known to be hepatic progenitor cells, were isolated from an adult rat liver. We found methods by which SHs could differentiate into mature hepatocytes(MHs) and form three-dimensional structures. SHs in a colony s...
The morphogenesis and movement of bile canaliculi (BC) are not well understood. This is because culture of hepatocytes that maintain polarity of cell membranes and possess highly differentiated functions has never been successful. We found that sm...
Small hepatocytes (SHs), which are known to be hepatic progenitor cells, were isolated from an adult rat liver. We found methods by which SHs could differentiate into mature hepatocytes and form three-dimensional structures. SHs in a colony someti...
This research program was composed of three components, namely the first is"the establishment extracorporeal hepatic tissue structure", the second is"the study of bile stasis under in vitro condition" and the third is "the treatment of hepatic fai...
[Background] It is envisaged that hepatocyte transplantation and extracorporeal liver support systems may be a future alternative therapy to orthotopic liver transplantation. Small hepatocytes known as liver hepatocyte stem cells would be consider...
We found three genes, CD44, BRI3, and D6.1A, with much higher expression in small hepatocytes (SHs)than in mature hepatocytes (MHs)and possessing transmembrane domain. Using specific antibody to CD44, BRI3, and D6.1A, we examined the localization ...
The aim of this study was to isolate and characterize human hepatic stem/progenitor cells from human liver and to reconstruct the liver tissue.Isolation method of hepatic stem progenitor cells from fetal and adult mise liver was established using ...
(1)It has been suggested that the mostly irregular contractions of bile canalicular net work constituted among the hepatoid structure by cultured small hepatocytes were. This movement was presented as calculated number by the specific-soft utiliza...
We have reported that CD44 is specifically expressed in rat small hepatocytes and that CD44^+ hepatocytes transiently appear in injured rat livers. Using a specific antibody, we sorted CD44^+ cells from galactosamine-treated rat livers and culture...
Laminins are a diverse group of alpha/beta/gamma heterotrimers formed from five alpha, three beta and three gamma chains ; they are major components of all basal laminae (BLs). One laminin chain that has garnered particular interest due to its wid...
The researches were composed of three kinds of contents as follows : (1) the studies about low localized molucules utilized for the assessments of hepatic function, (2) the regulation on isolation, identification and proliferation of hepatic stem ...
It is very important to perform surgical operation to obtain cell donation for cell transplantation therapy. Conventional hepatectomy has been considered to be difficult to decrease high mobidity with mortlity. A saline-linked electiric cautery wa...
The isolated cells rapidly lose viability and hepatic functions. Therefore, for development of culture systems for primary hepatocytes, effective substrata are required for both hepatocyte adhesion and maintenance of hepatic function. In this stud...
We aimed to develop the methods of producing a large number of small hepatocytes(SHs), hepatic progenitor cells, and to establish the methods of hepatic organoid formation in vitro. In addition, we examined how cells and tissues could be transplan...
We have achieved the results by the clinical and basic studies.【Study 1】We developed a rat model of acute liver failure after 2/3 partial hepatectomy (PH) for non-alcoholic steatohepatitis (NASH)-related cirrhosis. The mechanism by which HT improv...
