2017年8月16日
Development of an Activatable Fluorescent Probe for Prostate Cancer Imaging.
Bioconjugate chemistry
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- 巻
- 28
- 号
- 8
- 開始ページ
- 2069
- 終了ページ
- 2076
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.bioconjchem.7b00233
- 出版者・発行元
- AMER CHEMICAL SOC
Technology to visualize small prostate cancers is urgently needed because of the difficulty of discriminating prostate cancer from normal tissue with the naked eye, and a fluorescence imaging method would be advantageous. Here, we describe the design and synthesis of a fluorogenic probe (Ac-KQLR-HMRG) that is activated by hepsin and matriptase (proteases over-expressed in prostate cancer). Ac-KQLR-HMRG exhibited significant turn-on fluorogenicity in the presence of hepsin (180-fold) and matriptase (80-fold) and allowed specific fluorescence imaging of various prostate cancer cell line in vitro. In addition, the probe enabled rapid imaging (within 1-10 min) of small prostate cancer nodules in mouse models of disseminated peritoneal tumor and orthotopic tumor.
- リンク情報
- ID情報
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- DOI : 10.1021/acs.bioconjchem.7b00233
- ISSN : 1043-1802
- PubMed ID : 28691803
- Web of Science ID : WOS:000408075000008