2019年10月
Selective cross-linking behavior of oligodeoxyribonucleotides containing 2’-O-[N-(4,5’,8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine to mutant H-ras DNA Nucleosides
Nucleosides, Nucleotides & Nucleic Acids
- 巻
- 39
- 号
- 1-3
- 開始ページ
- 119
- 終了ページ
- 130
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/15257770.2019.1677912
Point mutations are well characterized activators of oncogenes but are often indistinguishable using common gene technologies. In general, the precise sites of point-mutated oncogenes are difficult to distinguish under physiological conditions primarily because single nucleotide mismatch do not affect the annealing temperatures of DNA probes sufficiently. To address this limitation, we developed photo-responsive oligodeoxyribonucleotides containing 2'-O-[N-(4,5',8-trimethylpsoralen-4'-ylmethylcarbamoyl)]adenosine (Ps-amd-Oligo), which can be used to selectively manipulate and identify genes with point mutations. Here we present time course analyses of the photo-cross-linking efficiency of Ps-amd-Oligo with DNA and RNA and show promising selectivity for the oncogene H-ras.
- リンク情報
- ID情報
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- DOI : 10.1080/15257770.2019.1677912
- PubMed ID : 31645189