2006年8月
Tetrameric hub structure of postsynaptic scaffolding protein Homer
JOURNAL OF NEUROSCIENCE
- ,
- ,
- 巻
- 26
- 号
- 33
- 開始ページ
- 8492
- 終了ページ
- 8501
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1523/JNEUROSCI.2731-06.2006
- 出版者・発行元
- SOC NEUROSCIENCE
Homer is a crucial postsynaptic scaffolding protein involved in both maintenance and activity-induced plasticity of the synapse. However, its quaternary structure has yet to be determined. We conducted a series of biophysical experiments that provide the first evidence that Homer forms a tetramer via its coiled-coil domain, in which all subunits are aligned in parallel orientation. To test the importance of the tetrameric structure for functionality, we engineered dimeric and tetrameric Homer by deleting a part of coiled-coil domain or replacing it with artificially engineered dimeric or tetrameric coiled-coil domain from a yeast protein. The structure - activity relationship was determined by assaying cocluster formation with its ligand in heterologous cells, distribution in dendritic spines, and turnover rate of protein exist in dendritic spines. Our results provide the first insight into the structure of native Homer protein as a tetramer and the functional significance conferred by that structure.
- リンク情報
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- DOI
- https://doi.org/10.1523/JNEUROSCI.2731-06.2006
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16914674
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000239817700009&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-33748126215&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-7560-3004
- ID情報
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- DOI : 10.1523/JNEUROSCI.2731-06.2006
- ISSN : 0270-6474
- ORCIDのPut Code : 7254016
- PubMed ID : 16914674
- Web of Science ID : WOS:000239817700009