2015年5月
Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element
PLOS ONE
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- 巻
- 10
- 号
- 5
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1371/journal.pone.0125354
- 出版者・発行元
- PUBLIC LIBRARY SCIENCE
The spatiotemporally controlled expression of G-CaMP fluorescent calcium indicator proteins can facilitate reliable imaging of brain circuit activity. Here, we generated a transgenic mouse line that expresses G-CaMP7 under a tetracycline response element. When crossed with a forebrain-specific tetracycline-controlled transactivator driver line, the mice expressed G-CaMP7 in defined cell populations in a tetracycline-controlled manner, notably in pyramidal neurons in layer 2/3 of the cortex and in the CA1 area of the hippocampus; this expression allowed for imaging of the in vivo activity of these circuits. This mouse line thus provides a useful genetic tool for controlled G-CaMP expression in vivo.
- リンク情報
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- DOI
- https://doi.org/10.1371/journal.pone.0125354
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/25946002
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422725
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000354049700062&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/25946002
- URL
- http://orcid.org/0000-0002-7560-3004
- ID情報
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- DOI : 10.1371/journal.pone.0125354
- ISSN : 1932-6203
- ORCIDのPut Code : 35932470
- PubMed ID : 25946002
- PubMed Central 記事ID : PMC4422725
- Web of Science ID : WOS:000354049700062