論文

査読有り 国際誌
2022年1月15日

Alpha-crystallin B chains in trastuzumab-resistant breast cancer cells promote endothelial cell tube formation through activating mTOR.

Biochemical and biophysical research communications
  • Lili Yang
  • ,
  • Kazuma Higashisaka
  • ,
  • Masafumi Shimoda
  • ,
  • Yuya Haga
  • ,
  • Naoki Sekine
  • ,
  • Hirofumi Tsujino
  • ,
  • Kazuya Nagano
  • ,
  • Kenzo Shimazu
  • ,
  • Yasuo Tsutsumi

588
開始ページ
175
終了ページ
181
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2021.12.056

The specific human epidermal growth factor receptor 2 (HER2)-targeting monoclonal antibody trastuzumab shows considerable clinical efficacy in patients with HER2-overexpressing breast cancer. However, about 20% of patients who receive trastuzumab in the adjuvant setting relapse, and approximately half of patients with metastatic HER2-positive breast cancer develop resistance to trastuzumab within 1 year. Although the mechanism of trastuzumab resistance has been explored broadly, whether and how angiogenesis participates in trastuzumab resistance is unclear. Here, we examined the association between angiogenesis and trastuzumab resistance by using a trastuzumab-resistant cell line (SKBR3-TR). Compared with that from the parental trastuzumab-sensitive SKBR3 cells, the culture supernatant from SKBR3-TR cells significantly increased the sprouting of endothelial cells. To identify intercellular features that contribute to the induction of endothelial tube formation, proteomics revealed that α-crystallin B chain (αB-crystallin) was upregulated in SKBR3-TR cells. Moreover, silencing of αB-crystallin significantly repressed SKBR3-TR-induced tube formation, and knockdown of αB-crystallin in SKBR3-TR cells suppressed the activation of mechanistic target of rapamycin (mTOR) in endothelial cells. In addition, treatment with rapamycin, an inhibitor of mTOR, reversed the SKBR3-TR-induced promotion of tube formation. In summary, αB-crystallin enhanced the ability of SKBR3-TR cells to activate mTOR in endothelial cells and thus promote angiogenesis.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2021.12.056
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34959190
ID情報
  • DOI : 10.1016/j.bbrc.2021.12.056
  • PubMed ID : 34959190

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